1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window

Villa, Irène; Vedda, A.; Cantarelli, Irene Xochilt; Pedroni, Marco; Piccinelli, Fabio; Bettinelli, Marco; Speghini, Adolfo; Quintanilla, Marta; Vetrone, Fiorenzo; Rocha, Uéslen; Jacinto, Carlos; Carrasco, Elisa; Rodríguez, Francisco Sanz; Juarranz, Ángeles; Rosal, Blanca del; Ortgies, Dirk H.; Gonzalez, Patricia Haro; Solé, J. Garcı́a; García, Daniel Jaque

doi:10.1007/s12274-014-0549-1

Cited by 196 publications

(148 citation statements)

References 88 publications

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“…1(c)). This result agrees with a recent study using 1,340-nm emitting nanoparticles for NIR-II fluorescence imaging [27]. However, this autofluorescence could still severely limit the signal-to-background ratio when imaging fluorophores with low quantum yield.…”

Section: Resultssupporting

confidence: 92%

Biological imaging without autofluorescence in the second near-infrared region

et al. 2015

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Fluorescence imaging is capable of acquiring anatomical and functional information with high spatial and temporal resolution. This imaging technique has been indispensable in biological research and disease detection/diagnosis. Imaging in the visible and to a lesser degree, in the near-infrared (NIR) regions below 900 nm, suffers from autofluorescence arising from endogenous fluorescent molecules in biological tissues. This autofluorescence interferes with fluorescent molecules of interest, causing a high background and low detection sensitivity. Here, we report that fluorescence imaging in the 1,500-1,700-nm region (termed "NIR-IIb") under 808-nm excitation results in nearly zero tissue autofluorescence, allowing for background-free imaging of fluorescent species in otherwise notoriously autofluorescent biological tissues, including liver. Imaging of the intrinsic fluorescence of individual fluorophores, such as a single carbon nanotube, can be readily achieved with high sensitivity and without autofluorescence background in mouse liver within the 1,500-1,700-nm wavelength region.

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Section: Resultssupporting

confidence: 92%

Biological imaging without autofluorescence in the second near-infrared region

et al. 2015

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“…Increasing the quantum yield, functionality, and biocompatibility of SWIR fluorophores is an active focus of emerging research studies (24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41).…”

Section: Significancementioning

confidence: 99%

Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green

Carr

Franke

Caram

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

593

569

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Fluorescence imaging is a method of real-time molecular tracking in vivo that has enabled many clinical technologies. Imaging in the shortwave IR (SWIR; 1,000–2,000 nm) promises higher contrast, sensitivity, and penetration depths compared with conventional visible and near-IR (NIR) fluorescence imaging. However, adoption of SWIR imaging in clinical settings has been limited, partially due to the absence of US Food and Drug Administration (FDA)-approved fluorophores with peak emission in the SWIR. Here, we show that commercially available NIR dyes, including the FDA-approved contrast agent indocyanine green (ICG), exhibit optical properties suitable for in vivo SWIR fluorescence imaging. Even though their emission spectra peak in the NIR, these dyes outperform commercial SWIR fluorophores and can be imaged in the SWIR, even beyond 1,500 nm. We show real-time fluorescence imaging using ICG at clinically relevant doses, including intravital microscopy, noninvasive imaging in blood and lymph vessels, and imaging of hepatobiliary clearance, and show increased contrast compared with NIR fluorescence imaging. Furthermore, we show tumor-targeted SWIR imaging with IRDye 800CW-labeled trastuzumab, an NIR dye being tested in multiple clinical trials. Our findings suggest that high-contrast SWIR fluorescence imaging can be implemented alongside existing imaging modalities by switching the detection of conventional NIR fluorescence systems from silicon-based NIR cameras to emerging indium gallium arsenide-based SWIR cameras. Using ICG in particular opens the possibility of translating SWIR fluorescence imaging to human clinical applications. Indeed, our findings suggest that emerging SWIR-fluorescent in vivo contrast agents should be benchmarked against the SWIR emission of ICG in blood.

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“…If that is the case, according to recently published works, autofluorescence removal requires the use of emission wavelengths longer than 1100 nm. 36 When working with Nd:NPs, this requirements implies to use only the 1.3 lm emission band for fluorescence contrast. Therefore, obtaining autofluorescencefree images of biological systems requires the use of NPs with large values of b J,13/2 .…”

Section: B Infrared Luminescencementioning

confidence: 99%

“…More recently, Villa et al used SrF 2 :Nd 3þ nanoparticles for autofluorescence-free deep tissue fluorescence imaging of a living mouse after intravenous injection of SrF 2 :Nd 3þ nanoparticles. 36 In this case, autofluorescencefree evidence of nanoparticle accumulation at both liver and spleen was obtained by recording the 1320 nm fluorescence ( 4 F 3/2 ! 4 I 13/2 transition) with an InGaAs camera (see Figure 1(c)).…”

Section: Introductionmentioning

confidence: 99%

Neodymium-doped nanoparticles for infrared fluorescence bioimaging: The role of the host

Rosal

Pérez‐Delgado

Misiak

et al. 2015

Journal of Applied Physics

Self Cite

113

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The spectroscopic properties of different infrared-emitting neodymium-doped nanoparticles (LaF 3 :Nd 3þ , SrF 2 :Nd 3þ , NaGdF 4 : Nd 3þ , NaYF 4 : Nd 3þ , KYF 4 : Nd 3þ , GdVO 4 : Nd 3þ , and Nd:YAG) have been systematically analyzed. A comparison of the spectral shapes of both emission and absorption spectra is presented, from which the relevant role played by the host matrix is evidenced. The lack of a "universal" optimum system for infrared bioimaging is discussed, as the specific bioimaging application and the experimental setup for infrared imaging determine the neodymiumdoped nanoparticle to be preferentially used in each case. V C 2015 AIP Publishing LLC.

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1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window

Cited by 196 publications

References 88 publications

Biological imaging without autofluorescence in the second near-infrared region

Biological imaging without autofluorescence in the second near-infrared region

Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green

Neodymium-doped nanoparticles for infrared fluorescence bioimaging: The role of the host

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