(1‐Butyl‐3‐methylimidazolium Hexafluorophosphate) Based Sensor for Quantification of Eugenol Antioxidant

Abstract

A simple, ultrasensitive and convenient electrochemical method has been developed for quantification of antioxidant eugenol, at multi‐walled carbon nanotube (MWCNT) ionic liquid (1‐Butyl 3‐methylimidazolium hexafluorophosphate) glassy carbon based sensor. The electrochemical behavior of eugenol at the sensor was studied using square wave voltammetry as a function of concentration of eugenol. The voltammetric studies of eugenol at MWCNT‐IL‐Gel/GC sensor exhibited a well defined anodic peak for its oxidation in Britton‐Robinson buffer at pH 10.5.Under optimized experimental condition the square wave oxidation peak current was linear over concentration range of 250 nM L−1 to 4000 nM L−1 with limit of detection (LOD) and limit of quantification of 87.6 nM L−1 and 292.2 nM L−1 respectively. The results indicate that the electrode coated with MWCNT‐IL‐Gel possess high sensitivity towards eugenol. Developed MWCNT‐IL‐Gel/GC sensor was successfully employed for the analysis of eugenol in real samples.

Ultrasensitive Voltammetric Quantification of Antioxidant Capsaicin at Platform Polypyrrole/Bi2O3/Graphene Oxide in Surfactant Stabilized Media

“…The data of three calibration curves were used for calculating standard deviation of intercepts and mean of slope. 30,31 Note: Frequency and Scan rate for linearity experiment were 25.00000 Hertz and 0.12500 Volt/second respectively.…”

“…Reproducibility.-Reproducibility of developed method was determined as per Guidance for Industry Bioanalytical Method Validation according to which "reproducibility of the method is assessed by replicate measurements using the assay, including quality controls". 30,32 In order to carry out reproducibility experiment known concentration of capsaicin (500 ng/mL) was run for five consecutive times and relative standard deviation for current response was calculated. According to guidance for Industry Bioanalytical Method Validation the relative standard deviation should be less than 15%.…”