1048 Gene Silencing of -catenin Inhibits Growth of Wnt/ß-catenin Pathway Dependent Colon Cancer Cell Lines

Bujak, Anna; Matłoka, Mikołaj; Mazur, Maria; Skupińska, Monika; Lamparska‐Przybysz, Monika; Wieczorek, Maria

doi:10.1016/s0959-8049(12)71659-3

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“…In their article, Yu et al introduced the role of myocardial adenylate-activated kinase AMPK in detail, pointed out that the myocardial adenylate-activated kinase AMPK is one of the important elements in human myocardial tissue, and explored the process and principle of AMPK inhibiting cardiomyocyte apoptosis [ 5 ]. Bujak et al clearly pointed out in their article that miR-451, as a noncoding RNA, can effectively enhance the activity of the AMPK signal in the pathway, emphasizing that the high level of miR-451 expression will inhibit the activity of the AMPK signal pathway, thereby exacerbating myocardial ischemia and reperfusion [ 6 ]. Wen et al introduced the mechanism of myocardial ischemia-reperfusion injury, they pointed out that although reperfusion therapy can treat a variety of vascular diseases, it is very easy to cause reperfusion injury; they emphasized that there is no good treatment for reperfusion injury [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

miR-451 on Myocardial Ischemia-Reperfusion in Rats by Regulating AMPK Signaling Pathway

Guo

Gao

Liu

et al. 2021

BioMed Research International

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Myocardial infarction is the main cause of death in patients with coronary heart disease. At present, the main method to treat cardiovascular disease is perfusion therapy. Myocardial ischemia-reperfusion will inevitably lead to reperfusion injury, which is also a major problem in the treatment of cardiovascular diseases. It has been reported that mir-451 in microRNA family participates in the protection of myocardial ischemia-reperfusion by regulating AMPK. The aim of this study was to investigate the effect of mir-451 on myocardial ischemia-reperfusion in rats by regulating AMPK signaling pathway. Sixty adult male rats were selected to establish myocardial ischemia-reperfusion animal model by ligating and loosening coronary artery. The expression level of mir-451 was regulated by injection of mir-451 virus vector and antibody, and the effect of increased or decreased mir-451 expression level on the activity of AMPK signaling pathway was detected. The myocardial infarct area and apoptosis rate of myocardial tissue were detected after 75 min ischemia-reperfusion. The results showed that when the expression level of mir-451 decreased by 15.7%, the activity index of AMPK signaling pathway was increased by 18.3%, the infarct area was reduced by 22.4%, and the apoptosis rate of myocardial cells was decreased by 25.2%. At the same time, the pathological structure of myocardial tissue was improved. Therefore, mir-451 is an inhibitor gene of AMPK signaling pathway. Reducing the expression of mir-451 can enhance the activity of AMPK signal pathway, and the increase of AMPK signal pathway activity is beneficial to reduce myocardial ischemia-reperfusion injury.

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Section: Introductionmentioning

confidence: 99%