1077 Anti-inflammatory and wound healing activities of calophyllolide isolated from Calophyllum inophyllum Linn

Abstract

Due to the high-cost and limitations of current wound healing treatments, the search for alternative approaches or drugs, particularly from medicinal plants, is of key importance. In this study, we report anti-inflammatory and wound healing activities of the major calophyllolide (CP) compound isolated from Calophyllum inophyllum Linn. The results showed that CP had no effect on HaCaT cell viability over a range of concentrations. CP reduced fibrosis formation and effectively promoted wound closure in mouse model without causing body weight loss. The underlying molecular mechanisms of wound repair by CP was investigated. CP markedly reduced MPO activity, and increased M2 macrophage skewing, as shown by up-regulation of M2-related gene expression, which is beneficial to the wound healing process. CP treatment prevented a prolonged inflammatory process by down-regulation of the pro-inflammatory cytokines-IL-1b, IL-6, TNF-a, but up-regulation of the antiinflammatory cytokine, IL-10. This study is the first to indicate a plausible role for CP in accelerating the process of wound healing through anti-inflammatory activity mechanisms, namely, by regulation of inflammatory cytokines, reduction in MPO, and switching of macrophages to an M2 phenotype. These findings may enable the utilization of CP as a potent therapeutic for cutaneous wound healing.

Caseinate-Stabilized Emulsions of Black Cumin and Tamanu Oils: Preparation, Characterization and Antibacterial Activity

“…Calophyllic acid and a lactone with antibiotic properties are the two main bioactive substances in the tamanu oil [9]. Calophyllum inophyllum is also known as a rich source of secondary metabolites (coumarins, xanthones, flavonoids, and triterpenes) [10], with some of them having anti-inflammatory, antibacterial, and antiviral properties [11,12]. Additionally, coumarin derivatives (calaustralin, calophyllolide, inophyllum, inophyllum E) that were obtained from a crude extract of the nuts were reported to have activity against Staphylococcus aureus [1] and antioxidant properties [13].…”