[117] Chromatographic determination of amino acids by the use of automatic recording equipment

Moore, Stanford; Stein, William H.

doi:10.1016/0076-6879(63)06257-1

Cited by 1,625 publications

(610 citation statements)

References 18 publications

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“…Amino acid analysis of the glucagon was carried out after hydrolysis according to Moore and Stein [7] and Matsubara and Sasaki [8].…”

Section: Amino Acid Analysismentioning

confidence: 99%

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Crystallization and amino acid sequence of duck glucagon

et al. 1972

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“…Amino acid analysis of the glucagon was carried out after hydrolysis according to Moore and Stein [7] and Matsubara and Sasaki [8].…”

Section: Amino Acid Analysismentioning

confidence: 99%

“…The yield of arginine is not corrected for losses in the hydrolytic procedure. In position 25, (a) Hydrolyzed in 6 N HC1 at 110 ° for 24 hr [7]. PTH-tryptophan was identified by gas chromatography, though in low yield.…”

Section: Sequence Determinationmentioning

confidence: 99%

Crystallization and amino acid sequence of duck glucagon

et al. 1972

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“…Amino acid analysis of the new compound was carried out, after hydrolysis with 6 N HCI at 105°C overnight [20], by HPLC (Waters, Manual no.CU85999, Milford, MA).…”

Section: Methodsmentioning

confidence: 99%

Characterization of vesicles, containing an acylated oligopeptide, released by human colon adenocarcinoma cells

Masella

Cantàfora

Guidoni³

et al. 1989

FEBS Letters

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RNA-containing vesicles, recovered from the supernatant of high-density cell samples of human colon carcinoma, produce a high-resolution 1H NMR spectrum of lipids characterized by isotropic tumbling; these vesicles contain large amounts of triglycerides and cholesterol esters. Both findings have strict analogies to what is displayed by the proteolipid complexes isolated from the sera of tumor-bearing patients [(1985) Proc. Natl. Acad. Sci. USA 82, 3455-3459; (1986) FEBS Lett. 203,. Lipid analysis and enzymatic tests indicate that these vesicles are selected micromaps of plasma membranes, analogous to those that can be recovered from culture media in which tumor cells are grown [(1985) Dev. Biol. 3, 33-57]. Peculiar lipids, an acylated oligopeptide and a modified phospholipid, are also present in the vesicles.

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“…quantitative estimation of glucosamine and galactosamine we recommend elution of the hydrolysate from the 23 cm column of the analyser with the standard pH 4.25 (0.2 M Na~ buffer of Moore and Stein [14] at 50°C. This fully resolves (in order) methione, isoleucine, leucine, tyrosine, phenylalanine, pfluorophenylalanine, glucosamine and galactosamine from each other and from tryptophan and its breakdown products which frequently interfere with the analysis of hexosamines in other systems such as that of Liu [4,5].…”

Section: Elson-morgan Reactionmentioning

confidence: 99%