buffer through the column, and the c hromatograph tubes are equipped with semiball connections.Columns of Dowex 50-X4 (9) also could be operated at faster flow rates in a similar manner.The separation of peptides on columns of Dowex 50-X2 (4, 7) can be speeded up by using a pump, -which should be placed in the line between the reservoir bottle or mixing chamber and the column. Chromatograph tubes suitable for use with higher pressure (IS) are then needed. The 2% cross-linked resin is available in a through-400-mesh grade (Technical Service and Development, Dow Chemical Co., Midland,

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