11α-Hydroxylation of 16α,17-epoxyprogesterone by Rhizopus nigricans in a biphasic ionic liquid aqueous system

Wu, Dazhuan; Guan, Yong; Wang, Haiqing; Yao, Shan‐Jing

doi:10.1016/j.biortech.2011.07.060

Cited by 45 publications

(35 citation statements)

References 24 publications

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“…Numerous studies have revealed that ILs were widely applied in pharmaceuticals to enhance steroid conversion . However, the influence and selection of ILs in whole‐cell biotransformation has not been thoroughly elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies have revealed that ILs were widely applied in pharmaceuticals to enhance steroid conversion. [17][18][19][20] However, the influence and selection of ILs in whole-cell biotransformation has not been thoroughly elucidated. This study explored the influence of 10 kinds of imidazolium-based ILs on the cellular growth rate, activity, and membrane permeability of A. simplex.…”

Section: Discussionmentioning

confidence: 99%

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Influence of imidazolium‐based ionic liquids on steroid biotransformation by Arthrobacter simplex

Wang

Liu

Tang

2017

J of Chemical Tech & Biotech

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BACKGROUND Ionic liquids (ILs) have become increasingly attractive as alternatives for organic solvents. In this study, the influences of ILs on the cellular growth rate, cellular activity, cortisone acetate (CA) conversion, CA solubility, and cell membrane permeability of Arthrobacter simplex CPCC 140451 (ASP) were examined. RESULTS Results showed that ILs with ≥3 carbon atoms in their side chains increased CA conversion, CA solubility, and cell membrane permeability of ASP and universally inhibited the cellular growth rate and activity, with ILs with [PF6]‐anion exhibiting the most severe inhibition activity. Inhibition was also more serious using ILs with longer alkyl side chains and at higher concentrations. CA conversion, CA solubility, and leakage of cell inclusions showed higher increase under ILs with [PF6]‐anion than those of ILs with [BF4]‐anion and lower increase for ILs with longer side chains. CONCLUSION This study improved the understanding of the influence and primary selection of imidazolium‐based ILs when applied in steroid biotransformation with Arthrobacter simplex whole cells. The data provided a foundation for the selection of suitable ILs for various applications. © 2017 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of imidazolium‐based ionic liquids on steroid biotransformation by Arthrobacter simplex

Wang

Liu

Tang

2017

J of Chemical Tech & Biotech

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“…However, the lower conversion rate of the biohydroxylation has become the bottleneck restricting the use of this fungus in potential industrial application [19]. Now in the large scale production, the conversion rate is about 50%.…”

Section: Discussionmentioning

confidence: 99%

“…11␣-Hydroxylation of 16␣, 17-epoxyprogesterone with R. nigricans is the main method to produce 11␣-hydroxy-16␣, 17-epoxyprogesterone for decades, which is a key step in the production of glucocorticoids [19], especially in China. How to improve the efficiency of hydroxylation has been the key aim in the large scale production.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, expression of CPR gene from Rhizopus oryzae into Rhizopus nigericans and its application in the 11α-hydroxylation of 16α, 17-epoxy-progesterone

Chen

Cao

et al. 2014

Enzyme and Microbial Technology

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“…Wu et al91 also investigated the 11α-hydroxylation of 16α,17-epoxyprogesterone (EP) by Rhizopus nigricans in an IL-containing biphasic medium as a means of improving yields from this industrially important biotransformation (Fig. 2c).…”

mentioning

confidence: 99%

Whole-Cell Biocatalytic Processes with Ionic Liquids

Zheng

et al. 2015

ACS Sustainable Chem. Eng.

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Whole-cell based biocatalysis in ionic liquids (ILs)-containing systems has attracted increasing interests in recent years. Compared to bioreactions catalyzed by isolated enzymes, the major advantages of using whole cell in biocatalytic processes are that cells provide a natural intracellular environment for the enzymes to function with cofactors regeneration in situ. An increasing number of renewable ILs are now accessible as a result of the ongoing progress in designing strategy of ILs and the sustainable environment requirement. The toxicity of ILs to microbial cells and the biodegradability are two of the crucial factors which allow the biocatalysis in ILs being applied in practice rather than in bench-scale. Applications of whole-cell biocatalysis in IL-containing system have, to date, been focused on the production of valuable compounds, mainly through reduction, oxidation and hydrolytic reactions. The mechanism research of ILs affecting the whole-cell biocatalysis offer the possibility to effectively integrate ILs with biotransformation. Thus, a comprehensive understanding of the whole cell-based biocatalytic process with ILs will contribute to the discovery of novel solvent for enzymatic reaction and the synthesis of more valuable compounds.

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11α-Hydroxylation of 16α,17-epoxyprogesterone by Rhizopus nigricans in a biphasic ionic liquid aqueous system

Cited by 45 publications

References 24 publications

Influence of imidazolium‐based ionic liquids on steroid biotransformation by Arthrobacter simplex

Influence of imidazolium‐based ionic liquids on steroid biotransformation by Arthrobacter simplex

Molecular cloning, expression of CPR gene from Rhizopus oryzae into Rhizopus nigericans and its application in the 11α-hydroxylation of 16α, 17-epoxy-progesterone

Whole-Cell Biocatalytic Processes with Ionic Liquids

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