129 Effects of Bacillus Subtilis on Growth Performance, Diarrhea, and Gut Health of Weanling Pigs Experimentally Infected with F18 Escherichia coli.

Kim, K; Jinno, Cynthia; Ehrlich, A; Li, X; Joergensen, Jens N; Raff, Lena; Liu, Y

doi:10.1093/jas/sky073.127

Cited by 3 publications

(2 citation statements)

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“…Larsen et al (2014) demonstrated that among 245 bacterial isolates, strains of B. amyloliquefaciens, B. subtilis, and B. mojavensis have the strongest probiotic potential. It was also reported that weaned pigs fed diets containing B. subtilis (DSM 25841) had improved growth rate and reduced gut permeability compared with pigs fed a control diet without B. subtilis (Kim et al, 2018), and weaned pigs infected with an enterotoxigenic F4+ Escherichia coli strain had reduced incidence of diarrhea when fed diets containing B. subtilis (DSM 25481) or B. amyloliquefaciens (DSM 25840;Röhe et al, 2017). As a consequence, B. amyloliquefaciens (DSM 25840) and B. subtilis (DSM 25841) were used in this experiment.…”

Section: Discussionmentioning

confidence: 98%

Effects ofBacillus amyloliquefaciensandBacillus subtilison ileal digestibility of AA and total tract digestibility of CP and gross energy in diets fed to growing pigs1

Blavi

Jørgensen

Stein

2018

Journal of Animal Science

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The objective of this experiment was to test the hypothesis that the apparent ileal digestibility (AID) of AA, CP, and GE, the apparent total tract digestibility (ATTD) of CP and GE, and the apparent hindgut digestibility of CP and GE by growing pigs may be improved by supplementing diets with two direct-fed microbials (DFM) containing different Bacillus strains. Twenty-four growing barrows (initial BW: 22.69 ± 1.48 kg) that had a T-cannula installed in the distal ileum were individually housed and randomly allotted to a three diet, three period design with 24 pigs and three 21-d periods. There were eight pigs per diet in each period for a total of 24 observations per diet. Three diets that were based on corn, soybean meal, and distillers dried grains with solubles were formulated. The control diet contained no DFM, but two additional diets contained two different Bacillus strains (Bacillus amyloliquefaciens or Bacillus subtilis). Feed was provided in mash form in two daily meals at 0800 and 1600 hours. The initial 12 d of each period was the adaptation period to the diet. Fecal and urine samples were collected from days 13 to 18, and ileal digesta were collected for 8 h on days 20 and 21. Results indicated that there were no differences among diets in ATTD of CP, but the AID of CP was reduced (P < 0.05) for the B. subtilis diet compared with control and B. amyloliquefaciens diets. Therefore, the apparent hindgut digestibility of CP was greater (P < 0.005) in pigs fed the B. subtilis diet compared with the other diets. The AID of total indispensable, total dispensable, and total AA was greater (P < 0.05) in the B. amyloliquefaciens diet compared with the control diet. There were no differences among diets in ATTD of GE, but the AID of GE was greater for the B. amyloliquefaciens diet than for the control and the B. subtilis diets (P < 0.001). Therefore, the apparent hindgut digestibility of GE was less (P < 0.05) in the B. amyloliquefaciens diet compared with the other diets. The DE (DM basis) for the B. subtilis diet was greater (P < 0.05) compared with the control and the B. amyloliquefaciens diets. In conclusion, supplementation of Bacillus spp. to diets fed to growing pigs may increase the AID of AA and GE, but there appears to be differences among strains of Bacillus spp. in their impact on AA and energy digestibility.

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Section: Discussionmentioning

confidence: 98%

Effects ofBacillus amyloliquefaciensandBacillus subtilison ileal digestibility of AA and total tract digestibility of CP and gross energy in diets fed to growing pigs1

Blavi

Jørgensen

Stein

2018

Journal of Animal Science

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“…147 Supplementation of β-glucan originated from different sources (yeast or algae) could enhance the resistance of pigs against ETEC infection. 62,148 The likely reasons may include enhanced gut integrity and health and reduced paracellular permeability, 149 reduced colonization of the small intestine with ETEC, 148 and boosted host immune response against ETEC infection. 62 Both dectin-1 and CR3 expressed on several immune cells (i.e., macrophages, neutrophils) are highly involved in the immuno-modulatory effects of β-glucan, 150 which need to be further elucidated.…”

Section: Prebiotics and Nondigestible Functional Carbohydratesmentioning

confidence: 99%

Dietary Factors in Prevention of PediatricEscherichia coliInfection: A Model Using Domestic Piglets

Liu¹,

2018

ILAR Journal

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Enterotoxigenic Escherichia coli (ETEC) is the major etiological agent causing acute watery diarrhea that is most frequently seen in young children in lower-income countries. The duration of diarrheal symptom may be shortened by antibiotic treatment, but ETEC is relative refractory to common antibiotics. Burgeoning evidence suggests bioactive components that naturally occur in human milk (e.g., lysozyme and oligosaccharides) and plants (e.g., nondigestible carbohydrates and phytochemicals) contain antimicrobial functions are promising preventive measures to control ETEC infection. Although the exact protective mechanisms may vary for each compound and are still not completely understood, they generally act to (1) competitively inhibit the binding of pathogenic bacteria and toxins to gut epithelium; (2) directly kill pathogens; and (3) stimulate and/or enhance host mucosal and systemic immune defense against pathogenic microorganisms. An appropriate ETEC-challenge animal model is critical to evaluate the effect and unveil the mechanism of bioactive compounds in prevention of enteric infection. Despite wide application in biomedical research, rodents do not usually manifest typical clinical signs of enteric infections. The remarkable differences in digestive physiology, immune response, and gut microbiota between rodents and human beings necessitate the use of alternative animal models. Pigs are closely related to humans in terms of genomes, physiology, anatomy of gastrointestinal tracts, digestive enzymes, components of immune system, and gut microbiota. Like human infants and young children, nursing and nursery piglets are more susceptible to ETEC infection and reproduce the clinical signs as observed in humans. Hence, the ETEC-challenge piglet represents a valuable translational model to study pathogenesis and evaluate dietary factors (e.g., milk bioactive compounds, nondigestible carbohydrates, and phytochemicals) as preventive measures for ETEC infection in pediatrics.

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Bacillus subtilis extracellular polymeric substances conditioning layers inhibit Escherichia coli adhesion to silicon surfaces: A potential candidate for interfacial antifouling additives

Hou

Suo

et al. 2021

Biointerphases

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Biofouling on material surfaces is a ubiquitous problem in a variety of fields. In aqueous environments, the process of biofouling initiates with the formation of a layer of macromolecules called the conditioning layer on the solid-liquid interface, followed by the adhesion and colonization of planktonic bacteria and the subsequent biofilm development and maturation. In this study, the extracellular polymeric substances (EPS) secreted by Bacillus subtilis were collected and used to prepare conditioning layers on inert surfaces. The morphologies and antifouling performances of the EPS conditioning layers were investigated. It was found that the initial adhesion of Escherichia coli was inhibited on the surfaces precoated with EPS conditioning layers. To further explore the underlying antifouling mechanisms of the EPS conditioning layers, the respective roles of two constituents of B. subtilis EPS (γ-polyglutamic acid and surfactin) were investigated. This study has provided the possibility of developing a novel interfacial antifouling additive with the advantages of easy preparation, nontoxicity, and environmental friendliness.

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129 Effects of Bacillus Subtilis on Growth Performance, Diarrhea, and Gut Health of Weanling Pigs Experimentally Infected with F18 Escherichia coli.

Cited by 3 publications

References 0 publications

Effects ofBacillus amyloliquefaciensandBacillus subtilison ileal digestibility of AA and total tract digestibility of CP and gross energy in diets fed to growing pigs1

Effects ofBacillus amyloliquefaciensandBacillus subtilison ileal digestibility of AA and total tract digestibility of CP and gross energy in diets fed to growing pigs1

Dietary Factors in Prevention of PediatricEscherichia coliInfection: A Model Using Domestic Piglets

Bacillus subtilis extracellular polymeric substances conditioning layers inhibit Escherichia coli adhesion to silicon surfaces: A potential candidate for interfacial antifouling additives

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