Effects ofBacillus amyloliquefaciensandBacillus subtilison ileal digestibility of AA and total tract digestibility of CP and gross energy in diets fed to growing pigs1

Abstract

The objective of this experiment was to test the hypothesis that the apparent ileal digestibility (AID) of AA, CP, and GE, the apparent total tract digestibility (ATTD) of CP and GE, and the apparent hindgut digestibility of CP and GE by growing pigs may be improved by supplementing diets with two direct-fed microbials (DFM) containing different Bacillus strains. Twenty-four growing barrows (initial BW: 22.69 ± 1.48 kg) that had a T-cannula installed in the distal ileum were individually housed and randomly allotted to a three diet, three period design with 24 pigs and three 21-d periods. There were eight pigs per diet in each period for a total of 24 observations per diet. Three diets that were based on corn, soybean meal, and distillers dried grains with solubles were formulated. The control diet contained no DFM, but two additional diets contained two different Bacillus strains (Bacillus amyloliquefaciens or Bacillus subtilis). Feed was provided in mash form in two daily meals at 0800 and 1600 hours. The initial 12 d of each period was the adaptation period to the diet. Fecal and urine samples were collected from days 13 to 18, and ileal digesta were collected for 8 h on days 20 and 21. Results indicated that there were no differences among diets in ATTD of CP, but the AID of CP was reduced (P < 0.05) for the B. subtilis diet compared with control and B. amyloliquefaciens diets. Therefore, the apparent hindgut digestibility of CP was greater (P < 0.005) in pigs fed the B. subtilis diet compared with the other diets. The AID of total indispensable, total dispensable, and total AA was greater (P < 0.05) in the B. amyloliquefaciens diet compared with the control diet. There were no differences among diets in ATTD of GE, but the AID of GE was greater for the B. amyloliquefaciens diet than for the control and the B. subtilis diets (P < 0.001). Therefore, the apparent hindgut digestibility of GE was less (P < 0.05) in the B. amyloliquefaciens diet compared with the other diets. The DE (DM basis) for the B. subtilis diet was greater (P < 0.05) compared with the control and the B. amyloliquefaciens diets. In conclusion, supplementation of Bacillus spp. to diets fed to growing pigs may increase the AID of AA and GE, but there appears to be differences among strains of Bacillus spp. in their impact on AA and energy digestibility.

Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics

“…Thus, the results of the present study support the hypothesis that the probiotic effect of Bacillus strains tested in the present trial can be ascribed to an improvement of feed digestibility by their enzymatic activity [56, 57]. This has also been confirmed by in vitro data showing secretion of NSP-enzymes and proteases by DSM25840 and DSM25841 [8] and by in vivo studies showing improved protein digestibility when these two strains were fed to grower-finisher pigs [58]. Despite an improvement of growth performance in probiotic groups was therefore expected in the present study, this result may be lack because only the most performing pigs were kept in the CO group.…”

Effects of Bacillus amyloliquefaciens and Bacillus pumilus on Rumen and Intestine Morphology and Microbiota in Weanling Jintang Black Goat

“…Therefore, the use of Bacillus as feed additives rather than antibiotics is justified in current research. An increasing number of Bacillus strains have been developed for use as animal feed additives [3,4].…”

Bacillus amyloliquefaciens CECT 5940 improves performance and gut function in broilers fed different levels of protein and/or under necrotic enteritis challenge

“… Farhat-Khemakhem et al (2018) reported that BA could ferment and hydrolyse complex carbohydrates and oligosaccharides, some being anti-nutrients such as non-starch polysaccharides, which leads to improved digestibility of feed components. Furthermore, the positive effects of BA supplementation on nutrient digestibility have also been previously observed in pigs and mice ( Blavi et al., 2019 ; Geeraerts et al., 2015 ). Positive alteration in gut microbiota, followed by improved digestibility of nutrients may be the underlying reason for the improved meat yield in this study.…”