13C labelled internal standards—A solution to minimize ion suppression effects in liquid chromatography–tandem mass spectrometry analyses of drugs in biological samples?

Berg, Thomas; Strand, Dag Helge

doi:10.1016/j.chroma.2011.10.081

Cited by 143 publications

(80 citation statements)

References 60 publications

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“…Use of the isotopically labeled analogue of the target analyte as an internal standard is the most effective way to rectify or account for matrix effects (Colby and McCaman 1979; Berg and Strand 2011). Theoretically, the same degree of ion suppression or enhancement will be observed for the target analyte and its isotopically labeled analogue.…”

Section: Controlling Matrix Effects In An Analytical Methodsmentioning

confidence: 99%

“…Theoretically, the same degree of ion suppression or enhancement will be observed for the target analyte and its isotopically labeled analogue. However, the ratio of the two signals should not be affected and correct quantification can still be achieved (Colby and McCaman 1979; Berg and Strand 2011). Unfortunately, isotopically labeled analogues are costly and often unavailable.…”

Section: Controlling Matrix Effects In An Analytical Methodsmentioning

confidence: 99%

“…Generally, the 13 C, 15 N, and 18 O labeled analogues are more similar to their native form than 2 H labeled analogues and are therefore expected to behave more similarly in chromatographic separations, resulting in the same retention time (Berg and Strand 2011). The 2 H labeled analogue has a stronger binding affinity than the 1 H isotope and has twice the atomic weight of its analogue and thus has a slightly different retention time than its native form in GC (Briscoe, Stiles et al 2007).…”

Section: Controlling Matrix Effects In An Analytical Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Biological Matrix Effects in Quantitative Tandem Mass Spectrometry-Based Analytical Methods: Advancing Biomonitoring

Panuwet

Hunter

DʼSouza

et al. 2015

Critical Reviews in Analytical Chemistry

290

190

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The ability to quantify levels of target analytes in biological samples accurately and precisely, in biomonitoring, involves the use of highly sensitive and selective instrumentation such as tandem mass spectrometers and a thorough understanding of highly variable matrix effects. Typically, matrix effects are caused by co-eluting matrix components that alter the ionization of target analytes as well as the chromatographic response of target analytes, leading to reduced or increased sensitivity of the analysis. Thus, before the desired accuracy and precision standards of laboratory data are achieved, these effects must be characterized and controlled. Here we present our review and observations of matrix effects encountered during the validation and implementation of tandem mass spectrometry-based analytical methods. We also provide systematic, comprehensive laboratory strategies needed to control challenges posed by matrix effects in order to ensure delivery of the most accurate data for biomonitoring studies assessing exposure to environmental toxicants.

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Section: Controlling Matrix Effects In An Analytical Methodsmentioning

confidence: 99%

Section: Controlling Matrix Effects In An Analytical Methodsmentioning

confidence: 99%

Section: Controlling Matrix Effects In An Analytical Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Biological Matrix Effects in Quantitative Tandem Mass Spectrometry-Based Analytical Methods: Advancing Biomonitoring

Panuwet

Hunter

DʼSouza

et al. 2015

Critical Reviews in Analytical Chemistry

290

190

View full text Add to dashboard Cite

show abstract

“…However, there are several limitations to mass spectrometry such as the potential for ion suppression caused by co-eluting ions from contaminating substances to yield false negative results (Hird et al, 2014;Kaufmann, Butcher, Maden, Walker, & Widmer, 2010). In this regard, stable isotope internal standards have been used to control ion suppression (Berg & Strand, 2011). Recently, high resolution mass spectrometry such as time of flight (Boix et al, 2014;Nacher-Mestre, Ibanez, Serrano, Perez-Sanchez, & Hernandez, 2013) and Orbitrap (Cepurnieks, Rjabova, Zacs, & Bartkevics, 2015;Li, Shen, Jiang, Huang, & Zhuo, 2013) has been successfully implemented to detect unknown metabolites or degradation products in wide-scope matrices.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of twenty-two sulfonamides in edible tissues: Investigation of new metabolites and their potential toxicity

Abdallah¹,

Arnaudguilhem²,

Haifa

et al. 2016

Food Chemistry

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“…SIL-IS are generally used to compensate for the impact of matrix effect on the analyte instrument response in each individual sample (23)(24)(25). They are chosen for a number of reasons.…”

Section: Introductionmentioning

confidence: 99%

Utilizing Internal Standard Responses to Assess Risk on Reporting Bioanalytical Results from Hemolyzed Samples

Fung

Aubry

Allentoff

et al. 2015

AAPS J

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Abstract. Bioanalytical analysis of toxicokinetic and pharmacokinetic samples is an integral part of small molecule drugs development and liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been the technique of choice. One important consideration is the matrix effect, in which ionization of the analytes of interest is affected by the presence of co-eluting interfering components present in the sample matrix. Hemolysis, which results in additional endogenous components being released from the lysed red blood cells, may cause additional matrix interferences. The effects of the degree of hemolysis on the accuracy and precision of the method and the reported sample concentrations from hemolyzed study samples have drawn increasing attention in recent years, especially in cases where the sample concentrations are critical for pharmacokinetic calculation. Currently, there is no established procedure to objectively assess the risk of reporting potentially inaccurate bioanalytical results from hemolyzed study samples. In this work, we evaluated the effect of different degrees of hemolysis on the internal standard peak area, accuracy, and precision of the analyses of BMS-906024 and its metabolite, BMS-911557, in human plasma by LC-MS/MS. In addition, we proposed the strategy of using the peak area of the stable isotope-labeled internal standard (SIL-IS) from the LC-MS/MS measurement as the surrogate marker for risk assessment. Samples with peak areas outside of the pre-defined acceptance criteria, e.g., less than 50% or more than 150% of the average IS response in study samples, plasma standards, and QC samples when SIL-IS is used, are flagged out for further investigation.KEY WORDS: hemolyzed sample/haemolyzed sample; hyperlipemic sample; internal standard peak area/internal standard responses; LC-MS/MS; regulated bioanalysis; risk assessment.

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13C labelled internal standards—A solution to minimize ion suppression effects in liquid chromatography–tandem mass spectrometry analyses of drugs in biological samples?

Cited by 143 publications

References 60 publications

Biological Matrix Effects in Quantitative Tandem Mass Spectrometry-Based Analytical Methods: Advancing Biomonitoring

Biological Matrix Effects in Quantitative Tandem Mass Spectrometry-Based Analytical Methods: Advancing Biomonitoring

Monitoring of twenty-two sulfonamides in edible tissues: Investigation of new metabolites and their potential toxicity

Utilizing Internal Standard Responses to Assess Risk on Reporting Bioanalytical Results from Hemolyzed Samples

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