13C Nmr Spectroscopy in Macromolecular Systems of Biochemical Interest

Rosenthal, Steven N.; Fendler, János H.

doi:10.1016/s0065-3160(08)60215-4

Cited by 44 publications

(17 citation statements)

References 399 publications

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“…Samples were again run in 5 mm tubes at 35 mC. Signals in the spectra were assigned by comparison with chemical shifts stated in the literature (Rosenthal & Fendler, 1976 ;Sprott et al, 1993 ;Pickett et al, 1994). For "H-NMR the concentration of a substrate and end product was established with reference to a standard (trimethylsilylpropionate, TSP) of known concentration, with the number of protons involved taken into account.…”

Section: Methodsmentioning

confidence: 99%

“…The predicted enrichments of key amino acids after growth on 2-["$C]pyruvate with an oxidative CAC in operation are : C-1 and C-4 for aspartate ; C-1 and C-5 for glutamate and C-2 for both alanine and glycine. The predicted enrichments for growth on 3-["$C]pyruvate are : C-2 and C-3 for aspartate ; C-2, C-3 and C-4 for glutamate ; C-3 for alanine and no labelling for glycine (Rosenthal & Fendler, 1976 ;Sprott et al, 1993 ;Pickett et al, 1994). Tables 1 and 2 respectively.…”

Section: C]pyruvatementioning

confidence: 99%

“…Tables 1 and 2 respectively. Individual peaks within the spectra were assigned according to previous data (Rosenthal & Fendler, 1976 ;Sprott et al, 1993 ;Pickett et al, 1994).…”

Section: C]pyruvatementioning

confidence: 99%

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An NMR and enzyme study of the carbon metabolism of Neisseria meningitidis

et al. 2001

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The pathogenic neisseriae are fastidious bacteria that are only able to grow on a restricted range of carbon sources. The genome sequence of Neisseria meningitidis strain MC58 predicts the presence of a complete citric acid cycle (CAC), but there have been no detailed biochemical studies of carbon metabolism in this important pathogen. In this study, both NMR and conventional enzyme assays were used to investigate the central metabolic pathways of a serogroup B strain (K454).13 C-NMR labelling patterns of amino acids from hydrolysed cell proteins after growth with either 2-or 3-[ 13 C]pyruvate were consistent with the operation of a complete oxidative CAC. Enzyme assays showed that cell-free extracts contained all the CAC enzymes predicted from the genome sequence, including a membrane-bound malate :quinone oxidoreductase which is present in place of the conventional NAD-linked cytoplasmic malate dehydrogenase. 1 H-NMR studies showed that growth on glucose, lactate and, especially, pyruvate, resulted in the excretion of significant amounts of acetate into the culture supernatant. This occurred via the phosphotransacetylase (PTA)-acetate kinase (ACK) pathway. Extremely high specific activities of PTA (7-14 µmol min N1 mg N1 ) were detected in cell-free extracts, although ACK activities were much lower (46-298 nmol min N1 mg N1 ). Expression of PTA and ACK activities was not co-ordinately regulated during growth on combinations of carbon sources. This may be related to the presence of two ackA paralogues in N. meningitidis which are, unusually, unlinked to the pta gene.

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Section: Methodsmentioning

confidence: 99%

Section: C]pyruvatementioning

confidence: 99%

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An NMR and enzyme study of the carbon metabolism of Neisseria meningitidis

et al. 2001

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“…The peak at 54.5 p.p.m. may be due to N-trimethyl groups of phospholipids (Rosenthal & Fendler, 1976), although solutes such as glycine-betaine (Norton, 1979), could also contribute to this resonance. If this peak did arise from phospholipids, it would be likely that the resonances arose from membrane lipids as well as triglycerides.…”

Section: Intact Myceziumentioning

confidence: 99%

“…$ Assignments taken from Rosenthal & Fendler (1976). dominated the spectrum of the fungus grown on sorbitol medium at 0.92 a, (Fig.…”

Section: Myceliat Extractsmentioning

confidence: 99%

Natural-abundance 13C Nuclear Magnetic Resonance Studies on the Internal Solutes of Xerophilic Fungi

Hocking

Norton

1983

Microbiology

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Natural-abundance 3C nuclear magnetic resonance spectroscopy was used to study the patterns of accumulation of osmotically active internal solutes in five different fungi. Four xerophilic fungi (Penicillium janczewskii, Eurotium chevalieri, Xeromyces bisporus and Wallemia sebi), and one non-xerophilic fungal species ( P . digitatum) were grown at three different water activities (a,) on media containing sorbitol, glucose/fructose or NaCl as the controlling solute. Under all conditions studied, the major internal solutes detected in aqueous ethanol extracts of these fungi were simple polyhydric alcohols : glycerol, erythritol, arabitol and mannitol. The most important osmoregulatory solute accumulated by all species was glycerol. On the sorbitol and the glucose/fructose media, all five fungi were able to accumulate glycerol. However, when NaCl was used to control a,, only one species, W. sebi, was able to accumulate glycerol below 0.92 a,, Significant quantities of the controlling solutes were also present in the extracts.When intact mycelia of P . janczewskii were examined by NMR, resonances of all the major internal solutes were clearly discernible, although they were not as well resolved as those from the fungal extracts. Relaxation measurements showed that the solutes were relatively mobile inside the cells.

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Salt‐induced conformational change of random copolymers (Lys⁵⁰Tyr⁵⁰)_n and (Lys⁵⁰Phe⁵⁰)_n studied by ¹H‐ and ¹³C‐nuclear magnetic resonances. Aggregation behavior of helical segments

Saitô¹,

Ohki²,

Kusumoto³

et al. 1979

Biopolymers

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Synopsis'H-and 13C-nmr studies of conformational transitions of random amino acid copolymers containing aromatic residues (Lys5'TyrS0), and ( L y~~~P h e~~) , in the presence of neutral salts were performed to serve as models of the aggregation behavior of polypeptides of biological significance. The 'Hand 13C signal intensities of Tyr and Phe residues decreased preferentially with increasing concentration of neutral salts such as NaCl and NaC104. This behavior contrasts with that of (Lys), in the presence of similar neutral salts, where the displacement of the I3C signal is clearly seen on transition from the random-coil to the helical conformation. On the basis of the previous conformational studies, the loss of the peak areas is ascribed to the presence of immobilized helical segments by hydrophobic interaction between aromatic side chains. The remaining resonances are due to the residual random-coil regions, since the values of nuclear Overhauser enhancements and chemical shifts are unchanged in the presence and absence of the neutral salts.

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13C Nmr Spectroscopy in Macromolecular Systems of Biochemical Interest

Cited by 44 publications

References 399 publications

An NMR and enzyme study of the carbon metabolism of Neisseria meningitidis

An NMR and enzyme study of the carbon metabolism of Neisseria meningitidis

Natural-abundance 13C Nuclear Magnetic Resonance Studies on the Internal Solutes of Xerophilic Fungi

Salt‐induced conformational change of random copolymers (Lys⁵⁰Tyr⁵⁰)_n and (Lys⁵⁰Phe⁵⁰)_n studied by ¹H‐ and ¹³C‐nuclear magnetic resonances. Aggregation behavior of helical segments

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13C Nmr Spectroscopy in Macromolecular Systems of Biochemical Interest

Cited by 44 publications

References 399 publications

An NMR and enzyme study of the carbon metabolism of Neisseria meningitidis

An NMR and enzyme study of the carbon metabolism of Neisseria meningitidis

Natural-abundance 13C Nuclear Magnetic Resonance Studies on the Internal Solutes of Xerophilic Fungi

Salt‐induced conformational change of random copolymers (Lys50Tyr50)n and (Lys50Phe50)n studied by 1H‐ and 13C‐nuclear magnetic resonances. Aggregation behavior of helical segments

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Salt‐induced conformational change of random copolymers (Lys⁵⁰Tyr⁵⁰)_n and (Lys⁵⁰Phe⁵⁰)_n studied by ¹H‐ and ¹³C‐nuclear magnetic resonances. Aggregation behavior of helical segments