13C-NMR study of the interaction of bacterial alginate with bivalent cations

Lattner, Daniel; Flemming, Hans‐Curt; Mayer, Christian

doi:10.1016/s0141-8130(03)00070-9

Cited by 97 publications

(70 citation statements)

References 15 publications

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“…These observed effects were bacteriostatic and may be related to the ability of alginates to chelate cations, particularly iron (32). G subunits are known to show preferential binding to cations such as Ca 2ϩ , although not all cations appear to utilize the G subunits as preferred binding sites (24). The magnitude of the antimicrobial effect was considerably potentiated using these lowmolecular-weight OligoG oligomers.…”

Section: Discussionmentioning

confidence: 97%

Overcoming Drug Resistance with Alginate Oligosaccharides Able To Potentiate the Action of Selected Antibiotics

Khan

Tøndervik

Sletta

et al. 2012

Antimicrob Agents Chemother

154

168

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eThe uncontrolled, often inappropriate use of antibiotics has resulted in the increasing prevalence of antibiotic-resistant pathogens, with major cost implications for both United States and European health care systems. We describe the utilization of a lowmolecular-weight oligosaccharide nanomedicine (OligoG), based on the biopolymer alginate, which is able to perturb multidrug-resistant (MDR) bacteria by modulating biofilm formation and persistence and reducing resistance to antibiotic treatment, as evident using conventional and robotic MIC screening and microscopic analyses of biofilm structure. OligoG increased (up to 512-fold) the efficacy of conventional antibiotics against important MDR pathogens, including Pseudomonas, Acinetobacter, and Burkholderia spp., appearing to be effective with several classes of antibiotic (i.e., macrolides, ␤-lactams, and tetracyclines). Using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), increasing concentrations (2%, 6%, and 10%) of alginate oligomer were shown to have a direct effect on the quality of the biofilms produced and on the health of the cells within that biofilm. Biofilm growth was visibly weakened in the presence of 10% OligoG, as seen by decreased biomass and increased intercellular spaces, with the bacterial cells themselves becoming distorted and uneven due to apparently damaged cell membranes. This report demonstrates the feasibility of reducing the tolerance of wound biofilms to antibiotics with the use of specific alginate preparations.

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Section: Discussionmentioning

confidence: 97%

Overcoming Drug Resistance with Alginate Oligosaccharides Able To Potentiate the Action of Selected Antibiotics

Khan

Tøndervik

Sletta

et al. 2012

Antimicrob Agents Chemother

154

168

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“…The polysaccharide pair mannuronate-guluronate was previously shown to be the preferred binding site for bivalent calcium ions in the bacterial alginate (Lattner et al, 2003). Moreover, xanthan, through its negatively charged GlcUA and ketal-pyruvate residues, is adsorbed significantly by magnesium carbonate, aluminum hydroxide, zinc oxide, and calcium carbonate, demonstrating its capacity to interact with suspended solids (Tempio and Zatz, 1981).…”

Section: Discussionmentioning

confidence: 99%

Xanthan Induces Plant Susceptibility by Suppressing Callose Deposition

et al. 2006

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Xanthan is the major exopolysaccharide secreted by Xanthomonas spp. Despite its diverse roles in bacterial pathogenesis of plants, little is known about the real implication of this molecule in Xanthomonas pathogenesis. In this study we show that in contrast to Xanthomonas campestris pv campestris strain 8004 (wild type), the xanthan minus mutant (strain 8397) and the mutant strain 8396, which is producing truncated xanthan, fail to cause disease in both Nicotiana benthamiana and Arabidopsis (Arabidopsis thaliana) plants. In contrast to wild type, 8397 and 8396 strains induce callose deposition in N. benthamiana and Arabidopsis plants. Interestingly, treatment with xanthan but not truncated xanthan, suppresses the accumulation of callose and enhances the susceptibility of both N. benthamiana and Arabidopsis plants to 8397 and 8396 mutant strains. Finally, in concordance, we also show that treatment with an inhibitor of callose deposition previous to infection induces susceptibility to 8397 and 8396 strains. Thus, xanthan suppression effect on callose deposition seems to be important for Xanthomonas infectivity.

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“…Spectroscopy, including NMR (Lattner et al, 2003), FTIR (Sheng et al, 2006), X ray photoelectron spectroscopy (XPS) (Ortega-Morales et al, 2007), and 3-dimensional excitation-emission matrix fluorescence spectroscopy (3D-EEM) (Sheng et al, 2006), can be used to elucidate functional groups and element compositions and to assess most of the linkages in EPS or microbial aggregates. NMR analysis allowed us to determine the functional groups of extracellular polysaccharide.…”

Section: Nmr Spectroscopic Characterization Of Epsmentioning

confidence: 99%

Characterization of Exopolysaccharides Produced by Rhizobia Species

Castellane

Otoboni

Lemos

2015

Rev. Bras. Ciênc. Solo

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increasing attention has been given, over the past decades, to the production of exopolysaccharides (eps) from rhizobia, due to their various biotechnological applications. overall characterization of biopolymers involves evaluation of their chemical, physical, and biological properties; this evaluation is a key factor in understanding their behavior in different environments, which enables researchers to foresee their potential applications. our focus was to study the eps produced by Mesorhizobium huakuii lMG14107, M. loti lMG6125, M. plurifarium lMG11892, Rhizobium giardini bv. giardini h152 t , R. mongolense lMG19141, and Sinorhizobium (= Ensifer) kostiense lMG19227 in a rdM medium with glycerol as a carbon source. these biopolymers were isolated and characterized by reversed-phase high-performance liquid chromatography (rp-hplC), fourier transform infrared (ftir), and nuclear magnetic resonance (nMr) spectroscopies. Maximum exopolysaccharide production was 3.10, 2.72, and 2.50 g l -1 for the strains LMG6125, LMG19227, and LMG19141, respectively. The purified EPS revealed prominent functional reactive groups, such as hydroxyl and carboxylic, which correspond to a typical heteropolysaccharide. the eps are composed primarily of galactose and glucose. Minor components found were rhamnose, glucuronic acid, and galacturonic acid. indeed, from the results of techniques applied in this study, it can be noted that the eps are species-specific heteropolysaccharide polymers composed of common sugars that are substituted by non-carbohydrate moieties. in addition, analysis of these results indicates that rhizobial EPS can be classified into five groups based on ester type, as determined

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13C-NMR study of the interaction of bacterial alginate with bivalent cations

Cited by 97 publications

References 15 publications

Overcoming Drug Resistance with Alginate Oligosaccharides Able To Potentiate the Action of Selected Antibiotics

Overcoming Drug Resistance with Alginate Oligosaccharides Able To Potentiate the Action of Selected Antibiotics

Xanthan Induces Plant Susceptibility by Suppressing Callose Deposition

Characterization of Exopolysaccharides Produced by Rhizobia Species

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