14 Days Repeat Oral Dose Toxicity of Low Molecular Weight Fucoidan in Rats

Abstract

-In order to investigate the preliminary repeat oral dose toxicity and to determine the highest dosage for further 4-week repeated dose toxicity test, Low Molecular Weight Fucoidan (LMF) has been showed various pharmacological effects, was orally administered to female and male rats, once a day for 14 days at dose levels of 2,000, 1,000, 500 and 0 (vehicle control) mg/kg (body weights) in a volume of 10 ml/kg. The mortality and changes on the body weights, clinical signs, hematology, serum biochemistry and gross observations were monitored with organ weight and histopathology of principle organs. As the results of 14-day repeated oral treatment of LMF, no LMF treatment related mortalities were detected up to 2,000 mg/kg in both male and female rats, respectively. In addition, no noticeable changes on the body weight and clinical signs were detected except for significant decreases on the body weights and gains restricted to male 2,000 mg/kg treated groups as compared with male vehicle control. No meaningful changes on the organ weights, hematological, serum biochemistrical, gross and histopathological findings were observed. Therefore the highest dosage in the 4-week repeated dose toxicity test is suggested as 2,000 mg/kg in both female and male rats, respectively.

Single Oral Dose Toxicity Study of Prebrewed Armeniacae Semen in Rats

“…Especially, the edematous changes in uterus were considered as secondary changes from different physiological estrus cycles (32 , 33) . In addition, most of them were also rarely but generally observed as sporadic accidental findings in rodent toxicity studies (5 , 23 , 24) .…”

“…Statistical analyses were conducted using SPSS for Windows (Release 14.0K, SPSS Inc., USA) and a p -value of less than 0.05 was considered to be a significant difference. In addition, degree of clinical signs, gross and histopathological findings were subdivided into 3 degrees: 3+ Severe, 2+ moderate, 1+ slight, according to the previous reports (5 , 23 , 24) .…”

Fucoidans: The importance of processing on their anti-tumoral properties

“…Additionally, after oral administration during 14 days, neither mortality nor hematological, histopathological and body and organs weights changes were found [30].…”