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Зиновьева, С. В.; Udalova, Zh. V.; Васильева, И. С.; Vanyushkin, S. A.; Paseshnichenko, V. A.

doi:10.1023/a:1010233721283

Cited by 10 publications

(7 citation statements)

References 10 publications

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“…It is generally believed that the physiological function of triterpenoids is defense (a plant’s acquired resistance) against plant pathogens 12. Plant‐derived pentacyclic triterpenoids of lupine and oleanane families provide a versatile structural platform for the discovery of new biologically active compounds (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Anti‐HCV Entry Activity Studies of β‐Cyclodextrin–Pentacyclic Triterpene Conjugates

Xiao

Wang

et al. 2014

ChemMedChem

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In our previous studies, oleanolic acid (OA) and echinocystic acid (EA), isolated from Dipsacus asperoides, were found to have anti-HCV entry properties. The major issue for members of this type of triterpene is their low water solubility. In this study, a series of new water-soluble triazole-bridged β-cyclodextrin (CD)-pentacyclic triterpene conjugates were synthesized via click chemistry. Thanks to the attached β-CD moiety, all synthesized conjugates showed lower hydrophobicity (Alog P) than their parent compounds. Several conjugates exhibited moderate anti-HCV entry activity. With the exception of per-O-methylated β-CD-pentacyclic triterpene conjugates, all other conjugates showed no cytotoxicity based on an alamarBlue assay carried out with HeLa, HepG2, MDCK, and 293T cells. More interestingly, the hemolytic activity of these conjugates disappeared upon the introduction of β-CDs. Easy access to such conjugates that combine the properties of β-CD and pentacyclic triterpenes may provide a way to obtain a new class of anti-HCV entry inhibitors.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Anti‐HCV Entry Activity Studies of β‐Cyclodextrin–Pentacyclic Triterpene Conjugates

Xiao

Wang

et al. 2014

ChemMedChem

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“…In fact, it is expected that wide gene modulation (as distinct from Torvum incompatible interaction) occurs due to the establishment of infection in eggplant. Indeed, compatible interaction involves complex rearrangements as development of multinucleate feeding sites, reallocation of nutritional resource to fulfill pathogen needs, root-galling circuitry and further morpho-physiological alterations to cope with enzyme-rich nematode secretions [31,32]. Indeed, more DEGs were found in compatible vs. incompatible interaction of Meloydogine spp.…”

Section: Resultsmentioning

confidence: 99%

“…Sesquiterpenoids include several compounds of well-established nematotoxic and nematostatic effects as gossypol, solavetivone, rishitin and lubumin [44], and references therein. Gossypol is a very effective sesquiterpene aldehyde shown to cause resistance to Meloydogine incognita [45,46] in cotton and solavetivone, rishitin and lubumin are bicyclic sesquiterpenoids expressed in solanaceous species as tomato and potato capable of causing nematostatic effects [31,33]. High content of solavetivone (associated to the H1 resistance gene) was found to be critical for conferring resistance to Globodera rostochiensis [47].…”

Section: Resultsmentioning

confidence: 99%

“…It should be noted, however, that a substantial number of plant phytochemicals exhibit antinematodal activity and that evidence on the antinematodal activity of sesquiterpenoids is largely based on in vitro studies [31,33,44,45]. Few studies monitored the accumulation of sesquiterpenoids upon elicitation and, in only a subset of them, as in cotton roots [45], elicitation was driven by M. incognita infection, more frequently, general and unspecific elicitors as arachidonic acid were employed.…”

Section: Resultsmentioning

confidence: 99%

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Solanum torvum responses to the root-knot nematode Meloidogyne incognita

et al. 2013

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BackgroundSolanum torvum Sw is worldwide employed as rootstock for eggplant cultivation because of its vigour and resistance/tolerance to the most serious soil-borne diseases as bacterial, fungal wilts and root-knot nematodes. The little information on Solanum torvum (hereafter Torvum) resistance mechanisms, is mostly attributable to the lack of genomic tools (e.g. dedicated microarray) as well as to the paucity of database information limiting high-throughput expression studies in Torvum.ResultsAs a first step towards transcriptome profiling of Torvum inoculated with the nematode M. incognita, we built a Torvum 3’ transcript catalogue. One-quarter of a 454 full run resulted in 205,591 quality-filtered reads. De novo assembly yielded 24,922 contigs and 11,875 singletons. Similarity searches of the S. torvum transcript tags catalogue produced 12,344 annotations. A 30,0000 features custom combimatrix chip was then designed and microarray hybridizations were conducted for both control and 14 dpi (day post inoculation) with Meloidogyne incognita-infected roots samples resulting in 390 differentially expressed genes (DEG). We also tested the chip with samples from the phylogenetically-related nematode-susceptible eggplant species Solanum melongena. An in-silico validation strategy was developed based on assessment of sequence similarity among Torvum probes and eggplant expressed sequences available in public repositories. GO term enrichment analyses with the 390 Torvum DEG revealed enhancement of several processes as chitin catabolism and sesquiterpenoids biosynthesis, while no GO term enrichment was found with eggplant DEG.The genes identified from S. torvum catalogue, bearing high similarity to known nematode resistance genes, were further investigated in view of their potential role in the nematode resistance mechanism.ConclusionsBy combining 454 pyrosequencing and microarray technology we were able to conduct a cost-effective global transcriptome profiling in a non-model species. In addition, the development of an in silico validation strategy allowed to further extend the use of the custom chip to a related species and to assess by comparison the expression of selected genes without major concerns of artifacts. The expression profiling of S. torvum responses to nematode infection points to sesquiterpenoids and chitinases as major effectors of nematode resistance. The availability of the long sequence tags in S. torvum catalogue will allow precise identification of active nematocide/nematostatic compounds and associated enzymes posing the basis for exploitation of these resistance mechanisms in other species.

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“…It is generally believed that one of the physiological functions of triterpenoids is to enhance the resistance of plants against the invasion of plant pathogens. 1 Triterpenoids exhibit a broad spectrum of pharmacological activities which are under active investigations worldwide for various therapeutic properties. [2][3][4] Ursolic acid (UA, 3β-hydroxy-urs-12-en-28-oic acid, 1) is a well-known ursane-type pentacyclic triterpenoid that has been reported to possess a wide range of biological activities, including antimicrobial, 5 antitumor, 2,6 antiviral, 7 antiprotozoal, 8 antioxidant, 9 anti-inflammatory, 10 antidiabetic 11 as well as anti-osteoporosic activities.…”

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confidence: 99%