Untitled

Abstract

The effects of sub-lethal doses of dichlorvos and formalin, antimicrobial/parasitic agents used in aquaculture, on lipid composition and metabolism of rainbow trout skin cells in primary culture were investigated. Both dichlorvos and, especially, formalin appeared to significantly inhibit Δ9 and Δ6 desaturase.Changes in the distribution of radioactivity between individual phospholipid classes was also influenced by formalin and dichlorvos, and this may be related to changes in desaturase activity. This study has shown that topically active agents used in aquaculture, formalin and dichlorvos, had a range of effects on the rainbow trout skin cell cultures that may affect cell proliferation and lipid and fatty acid metabolism. Both agents significantly inhibited desaturation of fatty acids, particularly of 18:2n-6 to 20:4n-6 and, as 20:4n-6 is a major eicosanoid precursor in fish and considering the importance of eicosanoids in the biochemistry of skin ,it is suggested that these agents may have direct effects on fish skin that may have important consequences for fish health in general.3

Fatty acid metabolism in marine fish: Low activity of fatty acyl Δ5 desaturation in gilthead sea bream (Sparus aurata) cells

“…The primary aim of the present study was to determine if the deficiency in the desaturase/elongase pathway, established in C [18][19][20] elongase in TF cells (11), was identical in another marine fish cell line. The results obtained using the direct substrates for each individual contrast to turbot cells, fatty acyl Δ5 desaturation was the step with the lowest activity in the pathway biosynthesizing 20:5n-3 (or 20:4n-6) in the sea bream cells.…”

“…This was unexpected as desaturases in general are normally more active towards n-3 fatty acids in mammals (32,33) and fish cells (10,11,34).…”

Histomorphological Studies of the Impact of Dichlorvos (Ddvp) on the Liver of Cyprinus Carpio, a Common Carp Fish

“…[4] The effects of sublethal doses of dichlorvos on lipid composition and metabolism of rainbow trout skin cells in primary culture suggested that dichlorvos might have direct effects on fish skin that could have important consequences for fish health. [5] In air-breathing catfish Clarias batrachus exposed to lethal and sublethal concentrations of the dichlorvos cytoarchitectural changes in the oocytes were observed. [6] Clumping of the cytoplasm and karyohypertrophy have also observed.…”