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Prabhasawat, Pinnita; Kosrirukvongs, Panida; Booranapong, Wipawee; Vajaradul, Yongyudh

doi:10.1023/a:1026542702099

Cited by 25 publications

(4 citation statements)

References 27 publications

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“…However, AM has both epithelialization and anti-inflammation properties; this has been demonstrated in previous clinical studies concerning AM transplantation [5-7]. Furthermore, AM has been shown to possess antibacterial properties [17,18].…”

Section: Discussionmentioning

confidence: 86%

“…In accordance with the tenets laid out in the Declaration of Helsinki and after having properly acquired informed consent, human AM was obtained during elective cesarean deliveries and processed as reported in a prior study [7]. In brief, AM was obtained from the placenta, which exhibited negative serological test results for the human immunodeficiency virus, hepatitis B and C viruses, and syphilis.…”

Section: Methodsmentioning

confidence: 99%

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Effects of amniotic membrane suspension in corneal epithelialization

Choi

2010

Acta Ophthalmologica

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PurposeThe main objective of this study is to determine the anti-inflammatory effect of topical amniotic membrane (AM) suspension on corneal alkali burn compared to topical serum eyedrops.MethodsThirty eyes from 30 Sprague-Dawley male rats were used. After alkali injuries using 1 N NaOH, the control group (n=10) received topical PBS four times a day for 2 days. The first study group received topical 30% AM suspension, and the second study group (n=10) received topical 30% rat serum. Using slit lamp biomicroscopy, injured corneas were evaluated and scored in terms of re-epithelialization, corneal opacity, and neovascularization (NV). Tissue sections were analyzed histologically for cellular infiltration, and immunohistochemical staining was conducted using rat anti-mouse F4/80 antibody for the detection of macrophages.ResultsIn the inflammatory wound healing model, the epithelial healing ratios of the control group, the AM suspension group, and the serum eyedrop group were 1.8±15.1%, 34.1±17.7%, and 41.5±16.1%, respectively (p<0.0001). The opacity scores for the control group, the AM suspension group, and the serum eyedrop group 48 h after the insult were 4.8±0.5, 3.4±0.5, and 3.0±0.8, respectively, showing a significant difference (p<0.0001). Moreover, the NV scores for the control group, AM suspension group, and serum eyedrop group 48 h after the insult were 5.8±0.9, 4.0±1.3, and 4.3±0.9 (p=0.006). Upon immunohistochemical evaluation using F4/80, significantly fewer F4/80+ cells were recruited in the AM suspension and serum eyedrop groups than the control group (p=0.027).ConclusionsThe suspension form of the amniotic membrane promoted epithelial healing and reduced corneal opacity and NV in alkali burn. It also suppressed F4/80 expression in the corneal stroma, indicating that the AM suspension maintains its beneficial biochemical effect on inflammatory corneal wound healing in vivo.

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Section: Discussionmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Effects of amniotic membrane suspension in corneal epithelialization

Choi

2010

Acta Ophthalmologica

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“…Furthermore, HAM has been used in clinics for the treatment of various problems, such as coverage of skin defects, anti-inflammation in burned skin or ulcers (25,26), corneal surface reconstruction (9,27), ocular surface reconstruction (17,28), control of wound infection (29), treatment of oral facial defects (30), bladder augmentation (31), and reconstruction of long ureteral defects (32). Based on properties of HAM, in this study it was decided to work on animals to evaluate the osteoinductive and osteoconductive effects of HAM.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Osteoinductive and Osteoconductive Effect of the Amniotic Membrane in Bone Defects due to Open Fractures in Rabbits

Moosavi¹,

Fakoor²,

Ahmad³

et al. 2018

J Orthop Spine Trauma

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Background: The repair of long bone segmental defects is one of the most challenging problems in orthopaedic surgery. Objectives: The current researchers carried out animal experiments on the use of Human Amniotic Membrane (HAM) in bone defect to evaluate the osteoinductive and osteoconductive effects, and also to use it as a guide for regular production of bone without waiting for membrane production (MASQUELET method). Methods: Twenty New Zealand white male adult rabbits were used in the study, and divided to four groups. The surgical site was prepared with the purpose of working on the left forearm diaphysis. In each radius, a bone defect of 15-mm in length was created. The fixation of the radius was not done because the support of the ulna was sufficient. The defect was not filled with anything in group 1; however, a tube-shaped HAM was inserted in group 2, a Tendon-shaped HAM was utilized for group 3 and a Tube-shaped HAM + bone graft (demineralized bone matrix, DBM) was used in group 4. Results: Bone formation was radiographically observed in the defects, which had been implanted using tube-shaped HAM (group 2), which was complete in 60% and partially complete in 40% of the cases. No bone formation was seen at up to eight weeks after surgery in group 1 and 3. A small amount of bone formation was observed at both ends and the ulnar site of the defect in group 4. Conclusions: The results of the study indicated that tube-shaped HAM could have an osteoconductive effect in large segmental bone defects, yet could not have an osteoinductive effect.

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“…In cryo condition AM manifests its capacity to promote migration and adhesion of corneal epithelium due to the presence of laminin 5 [15]. However, some safety prerequisites should be ensured to make cryopreserved AM as an ideal allograft for the treatment of different ophthalmic disorders [13] Microbial contamination of AM may occur due to the lack of aseptic techniques in tissue collection, procurement, and processing [16]. Sometimes microbial flora originated from a donor, operation theater or hospital personnel are responsible for microbial contamination of AM grafts.…”

Section: Introductionmentioning

confidence: 99%

Microbiological Quality Assessment at Different Stages of Frozen Amniotic Allograft Processing for Safe Tissue Banking Activities

Arifuzzaman¹,

Akhtar²,

Asaduzzaman³

2018

Cell Dev Biol

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Human Amniotic Membrane (HAM) is widely used as biological dressing material in reconstructive skin surgery, abdominal and vaginal reconstruction, plastic and cosmetic surgery and in ophthalmologic surgery. The objective of this study was to assess microbial quality of HAM during different stages of frozen HAM allograft processing and determination of antimicrobial susceptibility of isolated microorganisms. For this purpose, twelve amniotic sacs were collected from normal vaginal delivery of seronegative mother from Azimpur maternity, Dhaka, Bangladesh. Initial bioburden was determined by using Nutrient Agar (NA), McConkey Agar, Eosin Methylene Blue (EMB) Agar, Potato Dextrose Agar (PDA). Total Viable Bacterial Count (TVBC) was calculated and Initial bacterial load was ranged from 39 to 5.25 × 10 3. No fungus was found. A total 28 bacterial isolates were selected. These bacterial isolates were identified on the basis of cultural (e.g. colony size, shape, opacity), morphological (e.g. gram reaction, cell shape and arrangement) and biochemical characterization (e.g. catalase, oxidase, carbohydrate fermentation, Methyl Red (MR) test and Voges Proskauer (Vp) test). Of them, eight bacterial isolates were identified as Staphylococcus aureus, two were Staphylococcus epidermidis, nine were Escherichia coli, three were Salmonella typhimurium, one was Enterobacter aerogenes, one was Pseudomonas aeruginosa, four were Acinetobacter baumanii. Then, antimicrobial susceptibility pattern of isolated microorganisms were determined against ten antibiotics which includes Amphicillin, Streptomycin, Gentamycin, Neomycin, Imipenem, Vancomycin, Cloxacillin, Polymixin-B, Penicillin-G and Ciprofoxacin. It was found that, all bacterial isolates were sensitive to streptomycin and Penicillin-G. Thus, Streptomycin-Penicillin-G (Strep-P) cocktail was formulated and was used for the preparation of frozen AM. Then, bioburden was again determined by spread plate technique using the same media. Bacterial load in the processed HAM were ranged from 33 to 3.94 2. After then, HAM was preserved by using Dulbeco's Modified Eagles Media (DMEM) and glycerol (1:1 ratio) and was stored at-80°C. Microbial quality of the preserved samples were checked at 07, 14, 21 & 30 days and no bioburden was found. Thus, it can be said that the antibiotic cocktail was suitable to remove the culturable microorganisms associated with HAM.

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Untitled

Cited by 25 publications

References 27 publications

Effects of amniotic membrane suspension in corneal epithelialization

Effects of amniotic membrane suspension in corneal epithelialization

Evaluation of Osteoinductive and Osteoconductive Effect of the Amniotic Membrane in Bone Defects due to Open Fractures in Rabbits

Microbiological Quality Assessment at Different Stages of Frozen Amniotic Allograft Processing for Safe Tissue Banking Activities

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