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Rivas, Raúl; Velázquez, Encarna; Palomo, José-Luis; Mateos, Pedro F.; García-Benavides, Pablo; Martínez‐Molina, Eustoquio

doi:10.1023/a:1015044911913

Cited by 54 publications

(35 citation statements)

References 7 publications

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“…The genus Bradyrhizobium was proposed by Jordan (1982) for the slow-growing root nodule bacteria, in which colonies are generally less than or equal to 1 mm on yeast extract-mannitol agar (YMA) medium after 5-10 days incubation at 28 u C. At the time of writing, the genus contained 12 defined species: Bradyrhizobium japonicum (Jordan, 1982), B. elkanii (Kuykendall et al, 1992), B. liaoningense (Xu et al, 1995), B. yuanmingense (Yao et al, 2002), B. betae (Rivas et al, 2004), B. canariense (Vinuesa et al, 2005a), B. denitrificans (van Berkum et al, 2006), B. iriomotense (Islam et al, 2008), B. jicamae, B. pachyrhizi (Ramírez-Bahena et al, 2009), B. lablabi (Chang et al, 2011) and B. cytisi (Chahboune et al, 2011). With the exception of B. betae, all species are symbiotic nitrogenfixing bacteria associated with different legumes.…”

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“…1). Although the close phylogenetic relationships among the Bradyrhizobium species and the other related genera mentioned above have been found for a long time (Willems & Collins, 1992), several novel members of the genus Bradyrhizobium have been described based upon their close phylogenetic relationships with the defined Bradyrhizobium species, and their relationships with members of the genera Rhodopseudomonas, Afipia, Oligotropha and Nitrobacter were not considered (Ramírez-Bahena et al, 2009;Rivas et al, 2004;Vinuesa et al, 2005a).…”

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confidence: 99%

“…The novel group of four strains represented by CCBAU 15774 T could be allocated to the genus Bradyrhizobium based upon their closest phylogenetic relationships with the members in the major phylogenetic group and the criteria employed in the recent description of Bradyrhizobium species (Ramírez-Bahena et al, 2009;Rivas et al, 2004;Vinuesa et al, 2005a). Rhizobium lupini DSM 30140 T described by Ludwig et al (1995) should be reclassified in the genus Bradyrhizobium and Agromonas oligotrophica (Ohta & Hattori, 1983) might also be reclassified into this genus.…”

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confidence: 99%

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Bradyrhizobium daqingense sp. nov., isolated from soybean nodules

Wang

Zhang

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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Thirteen slow-growing rhizobial strains isolated from root nodules of soybean (Glycine max L.) grown in Daqing city in China were classified in the genus Bradyrhizobium based on 16S rRNA gene sequence analysis. Multilocus sequence analysis of IGS, atpD, glnII and recA genes revealed that the isolates represented a novel clade in this genus. DNA-DNA relatedness lower than 42.5 % between the representative strain CCBAU 15774 T and the type strains of the closely related species Bradyrhizobium liaoningense USDA 3622 T , Bradyrhizobium yuanmingense CCBAU 10071 T and Bradyrhizobium betae LMG 21987 T , further confirmed that this group represented a novel species. CCBAU 15774 T shared seven cellular fatty acids with the three above-mentioned species, but the fatty acids 15 : 0 iso and summed feature 5 (18 : 2v6,9c and/or 18 : 0 anteiso) were unique for this strain. The respiratory quinone in CCBAU 15774 T was ubiquinone-10 and the cellular polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, cardiolipin and unknown aminolipid, polar lipid and phospholipid. In addition, some phenotypic features could be used to differentiate the novel group from the related species. On basis of these results, we propose the name Bradyrhizobium daqingense sp. nov., with CCBAU 15774 T (5LMG 26137 T 5HAMBI 3184 T 5CGMCC 1.10947 T ) as the type strain. The DNA G+C content of the type strain is 61.2 mol% (T m ).

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Bradyrhizobium daqingense sp. nov., isolated from soybean nodules

Wang

Zhang

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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“…The genus includes root-nodulating bacteria, stem-nodulating bacteria with photosynthetic and nitrogen-fixing capacity, as well as non-symbiotic species, which occupy a variety of ecosystems (Eaglesham et al, 1990;Islam et al, 2008;Ormeño-Orrillo et al, 2013;Rivas et al, 2004). In the past few years, large biodiversity among strains of the genus Bradyrhizobium has been reported; however, the genus still encompasses few described species (Delamuta et al, 2012Germano et al, 2006;Helene et al, 2015;Hungria et al, 2015;Menna et al, 2009;Parker, 2015;Roma Neto et al, 2010).…”

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Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.

Delamuta

Ribeiro

Simões‐Araújo

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp. The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446 T , BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense, and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium. Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5 % nucleotide identity with other members of the genus Bradyrhizobium. Average nucleotide identity (ANI) of genome sequences between strain BR 446 T and B. huanghuaihaiense was below the threshold for species circumscription (90.7 %). DNA-DNA hybridization resulted in DT m values over 6.7 C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manausense than with B. huanghuaihaiense, indicating a different evolutionary history for nitrogen-fixation genes. Morpho-physiological, genotypic and genomic data supported that these BR strains represent a novel species for which the name Bradyrhizobium stylosanthis sp. nov. isAbbreviations: DDH, DNA-DNA hybridization; ITS, intergenic transcribed spacer; MLSA, multilocus sequence analysis.

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“…In this report, we describe two novel species belonging to the genus Paenibacillus that were isolated from human urine and cerebrospinal fluid (CSF) and which are closely related to Paenibacillus massiliensis, P. illinoisensis, P. xylanilyticus, P. barcinonensis, P. pabuli and P. amylolyticus (Rivas et al, 2005;Roux & Raoult, 2004;Sánchez et al, 2005;Shida et al, 1997a, b).…”

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confidence: 99%