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Gorshkov, N. G.; Izosimov, I. N.; Kazimov, A. A.; Kolychev, S. V.; Kudryashev, N. A.; Mashirov, L.G.; Rimskii-Korsakov, A. A.; Firsin, N. G.

doi:10.1023/a:1012801701488

Cited by 7 publications

(1 citation statement)

References 4 publications

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“…The mechanism of sodiated glycan gas phase fragmentation by collision-induced dissociation (CID) was investigated by the experimental MS and computational method. − Generally, sodium cationized glycans produce glycosidic and cross-ring fragmentations during the CID process. , The sodiated glycan gas phase fragmentation mechanism in MALDI ISD could share some similar route to the CID process, as ISD is believed to be a combination of the thermal (similar to CID) and radical driven fragmentation process . The role of OH – in the glycan fragmentation mechanism is not clearly understood, but the excited OH – ions trapped in between the NP matrix capping surface and analyte surface could possibly form OH radicals and further induce glycan ISD fragmentation with a preference on cross-ring fragmentations. The proposed mechanisms for Na + and OH – ion involvement in glycan fragmentation are shown in Figures b and c.…”

Section: Resultsmentioning

confidence: 99%

Surface Effects of Iron Oxide Nanoparticles on the MALDI In-Source Decay Analysis of Glycans and Peptides

Antone

Liang

Sherwood

et al. 2019

ACS Appl. Nano Mater.

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Inorganic nanoparticles as MALDI matrices have recently been explored to study the molecular mass determination and structural analysis of glycans and peptides. However, the specific factors contributing to the success of the analysis are not well elucidated. In this paper, we investigated the roles of nanoparticle surface coatings and additive ions in MALDI in-source decay (ISD) analysis of model glycans and peptides. Specifically, iron oxide nanoparticles with four defined capping molecules (gluconic acid, citric acid, lactobionic acid, or glutathione) were tested, and the roles of additives (NH4OH, NaOH, LiOH, NaCl, or trifluoroacetic acid) were examined. For a model glycan, maltoheptaose, and a model peptide, substance P acid, nanoparticle capping molecules, additive cations, and additive anions altogether influenced the molecular ion sensitivity and ISD fragmentation efficiency.

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