14C1, an antigen associated with human ovarian cancer, defined using a human IgG monoclonal antibody

Abstract

SUMMARYWe bave selected a buman EBV-transformed cell line from tbe involved lympb nodes of an ovarian cancer patient wbicb secretes an IgG 1 K antibody, able to recognize an antigen present on Ibe surface of ovarian cancer cells. The antigen, termed ' 14C1,' bas previously been shown by immunobistological tecbniques to be present on tbe surface of tbe malignant cells witbin tumour specimens. Western blotting analysis bas sbown Ibat tbe majority of primary ovarian cancer specimens and tbree continuous cell lines derived tberefrom express 14CI; otber tissue types were negative. Preliminary biocbemical cbaracterization bas been carried out, wbicb sbows tbat tbe 14CI antigen bas a molecular weight range of 25-32 kD and an isoelectric point from pf 6-3 to 6S. We believe that the 14C1 antigen is immunologically relevant to ovarian cancer patients and may tberefore represent a novel target for botb active and passive immunotherapy.

The development of a novel immunotherapy model of human ovarian cancer in human PBL-severe combined immunodeficient (SCID) mice

“…6). The ELISA protocol was shown to be specific for the detection of anti-Ovan-4 antibodies, as supernatants from the human EBV-transformed B cell line secreting antibody (IgG) with specificity for the human ovarian cancer-associated antigen termed 14C1 (as previously described by us [18]) consistently tested negative for anti-Ovan-4 reactivity in this assay. …”