16;21 Translocation in Acute Nonlymphocytic Leukemia with Abnormal Eosinophils: A Unique Subtype

Sadamori, Naoki; Yao, Ei-ichi; Tagawa, Masuko; Nakamura, Hideo; I, Sasagawa; Itoyama, Takahiro; Tokunaga, Seiji; Ichimaru, Michito; Nakamura, Isao; Kamei, Toshiaki; Yokoyama, Yasushi

doi:10.1159/000205069

Cited by 11 publications

(7 citation statements)

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“…Morphology of such cases usually show myelocytic and/or monocytic features; with few cases showing a prominent eosinophilic component, which was absent in our case. [17] The blasts in this case has a CD34+, C117+, CD13+ and CD33+ immunophenotype. Cases carrying this translocation have a dismal prognosis, as seen in our patient who succumbed to the disease within 3 months of diagnosis.…”

Section: Discussionmentioning

confidence: 75%

“…Cases carrying this translocation have a dismal prognosis, as seen in our patient who succumbed to the disease within 3 months of diagnosis. [17] We had an interesting case of a 62 year man who presented with fever, fatigue and mucosal bleeding. His peripheral blood had myeloblasts with absence of maturation, fitting into a FAB subtype of M1.…”

Section: Discussionmentioning

confidence: 99%

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Detection of recurrent balanced translocations in Acute Myeloid Leukemia by Multiplex RT-PCR

Madakshira

Kotwal²

2017

APALM

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Background: Diagnosis of acute myeloid leukemia (AML) has to be established by immunophenotyping and molecular testing for presence of recurrent genetic aberrations. This helps clinician to plan therapeutic protocol as AMLs are a heterogenous group of malignancies having varied response to treatment and prognosis. Multiplex Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) is a method capable of detecting 28 known fusion-transcripts of acute leukemia including cryptic translocations which would otherwise be missed on conventional cytogenetics. Methods:In this case series, a total of 19 consecutive patients diagnosed as AML were included. Morphology and cytochemical character of blasts were ascertained. Immunophenotying was carried out by flowcytometry. Messenager RNA (mRNA) was extracted from peripheral blood or bone marrow aspirates and converted to Complimentary DNA (cDNA). The cDNA was then subjected to multiplex RT-PCR for presence of fusion-transcripts using primers from Hemavision kit, DNA technologies, Denmark. The amplified product was correlated with the morphological, cytochemical and immunophenotypic character of AML. Result:Of the 19 cases of AML, 12 cases showed amplification of a fusion transcript. Most cases with fusion-transcripts showed an expected blast morphology and immunophenotype. Multiplex RT-PCR picked up presence of rare translocations such as t(16,21), t(9;22), t(6;9) and t(3;5), with unique clinicopathological features. Conclusion:Multiplex RT-PCR is a unique assay to identify multiple known recurrent genetic aberrations, including many splice variants. This aids in accurate subtyping of AML and helps the clinician to formulate a specific treatment plan for the patient.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Detection of recurrent balanced translocations in Acute Myeloid Leukemia by Multiplex RT-PCR

Madakshira

Kotwal²

2017

APALM

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“…The diagnosis of acute monocytic leukaemia, M4 or M5, was made for all three patients. The morphology of t(16;21) AML described in previous reports is heterogenous (Mecucci et al , 1985; Sadamori et al , 1990; Marosi et al , 1991; Morgan et al , 1991; Kong et al , 1997). However, most of the reported M1 and M2 cases with t(16;21) were described to be negative for CAE, which is recognized as specific for neutrophils.…”

Section: Discussionmentioning

confidence: 90%

“…The reciprocal translocation t(16;21)(p11;q22) is a rare abnormality demonstrated in acute myelocytic leukaemia (AML) (Mecucci et al , 1985; Yao et al , 1988; Minamihisamatsu & Ishihara, 1988; Sadamori et al , 1990; Shimizu et al , 1990; Morgan et al , 1991; Marosi et al , 1991; Nobbs et al , 1993; Hiyoshi et al , 1995; Yamamoto et al , 1997; Kong et al , 1997) and blast crisis of chronic myelocytic leukaemia (CML) (Ferro et al , 1992). About 50 cases of AML with t(16;21) have been reported to have various morphological features.…”

mentioning

confidence: 99%

“…About 50 cases of AML with t(16;21) have been reported to have various morphological features. Early reports described monocytic features of AML with t(16;21) such as M4 or M5 in the French–American–British (FAB) classification (Mecucci et al , 1985; Sadamori et al , 1990; Marosi et al , 1991), but recent reports have identified more heterogenous morphological features such as M1, M2, and M7 (Morgan et al , 1991; Kong et al , 1997).…”

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confidence: 99%

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Myeloid differentiation antigen and cytokine receptor expression on acute myelocytic leukaemia cells with t(16;21)(p11;q22): frequent expression of CD56 and interleukin‐2 receptor α chain

et al. 1999

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Summary.We report the cellular characteristics of cells from three patients with de novo acute myelocytic leukaemia (AML) with t(16;21)(p11;q22), two M4 and one M5a according to the FAB classification, and two permanent cell lines with t(16;21)(p11;q22), TSU1621MT and YNH-1. The FUS/ERG fusion mRNA was demonstrated in all cases by reverse transcriptase-polymerase chain reaction (RT-PCR). The immunophenotypes of the AML cells, and YNH-1 and TSU1621MT cell lines with t(16;21) were characterized as CD34Cells from all samples strongly expressed c-kit, granulocyte colony-stimulating factor receptor (G-CSFR), c-fms (macrophage colony-stimulating factor receptor), interleukin-3 receptor a chain (IL-3Ra), and granulocyte macrophage colony-stimulating factor receptor a chain (GM-CSFRa), and these data corresponded well to the growth responsiveness to the cytokines. IL-2Ra expression was also found in all t(16;21) samples, but IL-2 did not act on the proliferation of the leukaemic cells in in vitro cultures. G-CSF distinctly promoted the proliferation of leukaemic cells of t(16;21) AML, but did not enhance the expression of MPO and neutrophil differentiation of these cells. Our findings indicate that AML cells with t(16;21) preserve stem cell properties such as CD34 and c-kit expression, and suggest that they have the potential to differentiate into a monocytic lineage. The relationship between the unique cellular characteristics (especially CD56 and IL-2Ra expression) and FUS/ERG protein remains undetermined.

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Hemophagocytosis by leukemic blasts in 7 acute myeloid leukemia cases with t(16;21)(p11;q22)

Imashuku

Hibi

Shinoda

et al. 2000

Cancer

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16;21 Translocation in Acute Nonlymphocytic Leukemia with Abnormal Eosinophils: A Unique Subtype

Cited by 11 publications

References 7 publications

Detection of recurrent balanced translocations in Acute Myeloid Leukemia by Multiplex RT-PCR

Detection of recurrent balanced translocations in Acute Myeloid Leukemia by Multiplex RT-PCR

Myeloid differentiation antigen and cytokine receptor expression on acute myelocytic leukaemia cells with t(16;21)(p11;q22): frequent expression of CD56 and interleukin‐2 receptor α chain

Hemophagocytosis by leukemic blasts in 7 acute myeloid leukemia cases with t(16;21)(p11;q22)

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