[16] Mass culture of mammalian cells

McLimans, William F.

doi:10.1016/s0076-6879(79)58137-3

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…The effect of using an attachment period with intermittent stirring and reduced initial culture volume on the rate of attachment of vero-cells to cytodex-3 microcarrier leading good results due to modified procedure leading to more efficient utilization of the inoculum this procedure results in attachment efficiencies comparable to those observed in Petri dish. (McLimans, W.F, et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Optimizing culture conditions for increasing production of vero cell

El-Bagoury

El-Nahas

Abdelazeem

et al. 2018

Benha Veterinary Medical Journal

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The key to achieving maximum yields from microcarrier cultures is by using the optimization factors as replenishment of vero cell culture media during growth, the inoculation density on the proportion of microcarriers bearing cells, stirring speed on the growth of vero cell on cytodex 3, culture volume and headspace on cell yield from closed microcarrier system, various culture media, various types of the serum, control of PH, microcarrier concentration and modified initial culture procedure. The result reveled that the higher cell yield obtained after replenishment of culture media every three day, the optimum concentration of microcarrier is to be 3mg cytodex/ml, the optimum inoculation density 15x10 6 cells/mg cytodex microcarrier is required, the higher cell yield obtained by using (medium 199) containing high amount of amino acid at low density of the cell higher than Dulbecco's modification of Eagles's medium (DME) in higher density of the cell, fetal calf serum gave higher cell yield in the first three days in the culture, 60 rpm was the optimum stirring speed to get higher cell yield, addition of 10-25 mμ Hepes helped in maintaining the PH around 7.3-7.4 this gave higher cell yield, reduced the head space not more than half full of the culture give a higher result and the modified in initial culture procedure during the growth culture give higher results. The obtained result maximizing the yield of the vero cell production on acytodex-3 microcarrier culture system.

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Section: Discussionmentioning

confidence: 99%

Optimizing culture conditions for increasing production of vero cell

El-Bagoury

El-Nahas

Abdelazeem

et al. 2018

Benha Veterinary Medical Journal

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“…These additions can include buffers (HEPES), acid (HCI), base (NaOH) and nutrients. Addition of NaCl and the correct amount required to achieve a particular osmolarity is calculated as follows (232): The osmolarity of the medium is measured and the amount of stock NaCl (1 mg/mL) that must be added to achieve the desired osmolarity is calculated.…”

Section: Osmolaritymentioning

confidence: 99%

“…The ideal replenishment scheme is the one that results in the smallest fluctuation of nutrient concentrations and pH during the culture cycle. For this reason, a continuous flow of medium is the preferred method for culture maintenance (232,256). However, for small-scale cultures or experiments with cell densities up to 3-5×10 6 cells/mL batch, medium replenishment is more convenient.…”

Section: Replenishment Of Culture Mediummentioning

confidence: 99%

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Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

Wehlage¹,

Blattner²,

Mamun³

et al. 2020

AIMS Bioengineering

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“…The steady-state culture system [5][6][7][8] was pro grammed at pre-optimized levels for human epider mis [7], Human split thickness skin was minced and inoculated as described [7], Culture medium was freshly prepared for each experiment, and stored at -2 0 °C to minimize ammonia generation [7,9].…”

Section: Culture Methodsmentioning

confidence: 99%

Cultured Human Skin: Heterotransplantation

1982

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We have reported that human skin, cultured in a controlled environment system (steady state), yields epidermis-like growth from adult donor split thickness specimens. A procedure for transplantation of such cellular outgrowths to athymic nude mice for functional and morphologic evaluations is reported. Successful transplants were definitively scored by the presence of histologic epidermal markers and human glucose phosphate isomerase. We suggest that the transplantation procedures reported here may contribute to the knowledge required for the use of autologous cultured skin in patients with large skin wounds.

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[16] Mass culture of mammalian cells

Cited by 15 publications

References 19 publications

Optimizing culture conditions for increasing production of vero cell

Optimizing culture conditions for increasing production of vero cell

Cell growth on electrospun nanofiber mats from polyacrylonitrile (PAN) blends

Cultured Human Skin: Heterotransplantation

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