18F-FDG labelling of hematopoietic stem cells: Dynamic study of bone marrow homing by PET–CT imaging and impact on cell functionality

Faivre, Lionel; Chaussard, Michael; Vercellino, Laëtitia; Hosten, Benoît; Teixera, K.; Parietti, Véronique; Merlet, Pascal; Sarda‐Mantel, Laure; Rizzo-Padoin, N.; Larghero, Jérôme

doi:10.1016/j.retram.2016.06.002

Cited by 10 publications

(31 citation statements)

References 38 publications

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“…One of the most commonly applied clinical PET cell tracking approaches in cardiovascular studies uses direct cell labelling with 2′-[ 18 F]-fluoro-2′-deoxy-D-glucose ( 18 F-FDG)89111213141516. 18 F-FDG is a glucose analogue which becomes phosphorylated and trapped intracellularly and therefore can be used as a marker of metabolic activity as well as for cell tracking.…”

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confidence: 99%

PET Cell Tracking Using 18F-FLT is Not Limited by Local Reuptake of Free Radiotracer

MacAskill

Tavares

et al. 2017

Sci Rep

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Assessing the retention of cell therapies following implantation is vital and often achieved by labelling cells with 2′-[18F]-fluoro-2′-deoxy-D-glucose (18F-FDG). However, this approach is limited by local retention of cell-effluxed radiotracer. Here, in a preclinical model of critical limb ischemia, we assessed a novel method of cell tracking using 3′-deoxy-3′-L-[18F]-fluorothymidine (18F-FLT); a clinically available radiotracer which we hypothesise will result in minimal local radiotracer reuptake and allow a more accurate estimation of cell retention. Human endothelial cells (HUVECs) were incubated with 18F-FDG or 18F-FLT and cell characteristics were evaluated. Dynamic positron emission tomography (PET) images were acquired post-injection of free 18F-FDG/18F-FLT or 18F-FDG/18F-FLT-labelled HUVECs, following the surgical induction of mouse hind-limb ischemia. In vitro, radiotracer incorporation and efflux was similar with no effect on cell viability, function or proliferation under optimised conditions (5 MBq/mL, 60 min). Injection of free radiotracer demonstrated a faster clearance of 18F-FLT from the injection site vs. 18F-FDG (p ≤ 0.001), indicating local cellular uptake. Using 18F-FLT-labelling, estimation of HUVEC retention within the engraftment site 4 hr post-administration was 24.5 ± 3.2%. PET cell tracking using 18F-FLT labelling is an improved approach vs. 18F-FDG as it is not susceptible to local host cell reuptake, resulting in a more accurate estimation of cell retention.

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mentioning

confidence: 99%

PET Cell Tracking Using 18F-FLT is Not Limited by Local Reuptake of Free Radiotracer

MacAskill

Tavares

et al. 2017

Sci Rep

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“…Although the activity is usually in the range of a few Bq per cell for standard labelling protocols (e.g. [20], [21]), it was already demonstrated that high activity levels in the range of 50-70 Bq/cell could be achieved using F-18-FDG or Ga-68 labelled cancer cells [10], [22]. PET isotopes with short half-life would be preferable for tracking fast moving cells as less molecules need to be attached to the cell providing high activity.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Cell Imaging in PET With Optimal Transport Regularization

Schmitzer

Schäfers

Wirth

2020

IEEE Trans. Med. Imaging

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We propose a novel dynamic image reconstruction method from PET listmode data that could be particularly suited to tracking single or small numbers of cells. In contrast to conventional PET reconstruction our method combines the information from all detected events not only to reconstruct the dynamic evolution of the radionuclide distribution, but also to improve the reconstruction at each single time point by enforcing temporal consistency. This is achieved via optimal transport regularization where in principle, among all possible temporally evolving radionuclide distributions consistent with the PET measurement, the one is chosen with least kinetic motion energy. The reconstruction is found by convex optimization so that there is no dependence on the initialization of the method. We study its behaviour on simulated data of a human PET system and demonstrate its robustness even in settings with very low radioactivity. In contrast to previously reported cell tracking algorithms, our technique is oblivious to the number of tracked cells. Without any additional complexity one or multiple cells can be reconstructed, and the model automatically determines the number of particles. For instance, four radiolabelled cells moving at a velocity of 3.1 mm/s and a PET recorded count rate of 1.1 cps (for each cell) could be simultaneously tracked with a tracking accuracy of 5.3 mm inside a simulated human body.

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“…Its rate of cellular uptake correlates with the cell's metabolic rate; however, for SC imaging it is used as a label before grafting [40]. Although it provides high sensitivity and low cytotoxicity, its half-life of 110 min makes it less suitable for longitudinal studies to monitor SC biodistribution and homing [12,41].…”

Section: Advantages and Challenges Of Sc Imaging Methods Radionuclidementioning

confidence: 99%

Accomplishments and challenges in stem cell imaging in vivo

Bose

Mattrey

2019

Drug Discovery Today

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18F-FDG labelling of hematopoietic stem cells: Dynamic study of bone marrow homing by PET–CT imaging and impact on cell functionality

Cited by 10 publications

References 38 publications

PET Cell Tracking Using 18F-FLT is Not Limited by Local Reuptake of Free Radiotracer

PET Cell Tracking Using 18F-FLT is Not Limited by Local Reuptake of Free Radiotracer

Dynamic Cell Imaging in PET With Optimal Transport Regularization

Accomplishments and challenges in stem cell imaging in vivo

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