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A membrane‐associated creatine kinase (EC 2.7.3.2) identified as an acidic species of the non‐receptor, peripheral v‐proteins in Torpedo acetylcholine receptor membranes

“…The 2-dimensional system in [22] was used without modifications. Integration of the Coomassie Blue-stained spots was done after digitaiisation of the data acquired with the aid of a high resolution vidicon camera.…”

The 66 kDa component of eukaryotic initiation factor 3 interacts with globin mRNA and 18 S rRNA in preinitiation complexes

“…For this purpose the 66 kDa protein was labelled by iodination within the 22 S complex and coelectrophoresed with unlabelled eIF-3 from rabbit reticulocytes using the system described by O'Farrell [9]. The labelled spot with an apparent molecular mass of 66 kDa was superimposed with the 66 kDa subunit of eIF-3, which was localized by silver staining ( fig.1).…”

“…The precipitate was dried in vacuum and dissolved in 20 ~1 lysis buffer containing 9.6 M urea, 2% NP-40, 2% Pharmalyte 3-10 (Pharmacia, Sweden) and 0.1 M mercaptoethanol. eIF-3 from rabbit reticulocytes (50ag), isolated as described in [8], was added and the proteins were separated using the two-dimensional system described in [9].…”

Biochemical properties of attachment region binding protein ARBP

“…*Corresponding author.Fax: (49) (40) 4717 4862. Two-dimensional polyacrylamide gel electrophoresis was performed using the O'Farrell minigel system [15,16] with p1 markers from BioRad (hen egg white conalbumin, 6.66.6; bovine muscle actin, 5.5; bovine carbonic anhydrase, 5.96.0).…”