1BL.1RS translocation in some Indian bread wheat genotypes and strategies for its use in future wheat breeding

Kumar, Sachin; Kumar, Neeraj; Balyan, H. S.; Gupta, P. K.

doi:10.1080/00087114.2003.10589303

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…Physical mapping of specific loci including rRNA genes has also been achieved through FISH (Yamamoto and Mukai, 1995). Similarly, the alien chromatin, introgressed in wheat, could be successfully detected using GISH and FISH Schwarzacher et al, 1992;Kumar et al, 2003).…”

Section: Physical Mapping Using Fish and Gishmentioning

confidence: 96%

“…One of the most important examples of the use of alien chromatin in wheat improvement is the 1BL.1RS translocation (for related references, see Kumar et al, 2003). Molecular markers specific to chromosome arms 1BL and 1BS of wheat and 1RS of rye were used for the identification of this translocation Nagy and Lelley, 2003).…”

Section: Molecular Markers and Alien Gene Transfermentioning

confidence: 99%

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Wheat cytogenetics in the genomics era and its relevance to breeding

Gupta

Kulwal

Rustgi³

2005

Cytogenet Genome Res

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Hexaploid wheat is a species that has been subjected to most extensive cytogenetic studies. This has contributed to understanding the mechanism of the evolution of polyploids involving diploidization through genetic restriction of chromosome pairing to only homologous chromosomes. The availability of a variety of aneuploids and the ph mutants (Ph1 and Ph2) in bread wheat also allowed chromosome manipulations leading to the development of alien addition/substitution lines and the introgression of alien chromosome segments into the wheat genome. More recently in the genomics era, molecular tools have been used extensively not only for the construction of molecular maps, but also for identification/isolation of genes/QTLs (including epistatic QTLs, eQTLs and PQLs) for several agronomic traits. It has also been possible to identify gene-rich regions and recombination hot spots in the wheat genome, which are now being subjected to sequencing at the genome level, through development of BAC libraries. In the EST database also, among all plants wheat ESTs are the highest in number, and are only next to those for human, mouse, Ciona intestinalis (a chordate), rat and zebrafish genomes. These ESTs and sequences of several genomic regions have been subjected to a variety of applications including development of perfect markers and establishment of microcollinearity. The technique of in situ hybridization (including FISH, GISH and McFISH) and the development of deletion stocks also facilitated the preparation of physical maps. Molecular markers are also used for marker-assisted selection in wheat breeding programs in several countries. Construction of a wheat DNA chip, which will also become available soon, may further facilitate wheat genomics research. These enormous resources, knowledge base and the fast development of additional molecular tools and high throughput approaches for genotyping will prove extremely useful in future wheat research and will lead to development of improved wheat cultivars.

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Section: Physical Mapping Using Fish and Gishmentioning

confidence: 96%

Section: Molecular Markers and Alien Gene Transfermentioning

confidence: 99%

Wheat cytogenetics in the genomics era and its relevance to breeding

Gupta

Kulwal

Rustgi³

2005

Cytogenet Genome Res

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“…The rye (Secale cereale L.) derived wheat genotypes, also known as the 1B/1R chromosome translocation lines, have been utilized by different wheat breeding programs (Kumar et al, 2003;Hoffmann, 2008;Ren et al, 2017). These 1B/1R chromosome translocation genotypes consist of the long arm of wheat chromosome 1B including its centromere, and the short arm or a portion of rye chromosome 1R (Cai and Liu, 1989;Heslop-Harrison et al, 1990).…”

Section: Utilization Of Genetic Variation From Diverse Sourcesmentioning

confidence: 99%

Recent Progress in Germplasm Evaluation and Gene Mapping to Enable Breeding of Drought-Tolerant Wheat

2020

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There is a need to increase wheat productivity to meet the food demands of the evergrowing human population. However, accelerated development of high yielding varieties is hindered by drought, which is worsening due to climate change. In this context, germplasm diversity is central to the development of drought-tolerant wheat. Extensive collections of these genetic resources are conserved in national and international genebanks. In addition to phenotypic assessments, the use of advanced molecular techniques (e.g., genotype by sequencing) to identify quantitative trait loci (QTLs) for drought tolerance related traits is useful for genome-and marker-assisted selection based approaches. Therefore, to assist wheat breeders at a critical time, we searched the recent peer-reviewed literature (2011-current), first, to identify wheat germplasm observed to be useful genetic sources for drought tolerance, and second, to report QTLs associated with drought tolerance. Though many breeders limit the parents used in breeding programs to a familiar core collection, the results of this review show that larger germplasm collections have been sources of useful genes for drought tolerance in wheat. The review also demonstrates that QTLs for drought tolerance in wheat are associated with diverse physio-morphological traits, at different growth stages. Here, we also briefly discuss the potential of genome engineering/editing to improve drought tolerance in wheat. The use of CRISPR-Cas9 and other gene-editing technologies can be used to fine-tune the expression of genes controlling drought adaptive traits, while high throughput phenotyping (HTP) techniques can potentially accelerate the selection process. These efforts are empowered by wheat researcher consortia.

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“…Rye ( Secale cereale L.) is an important gene source for wheat ( Triticum aestivum L.) cultivar improvement. Only the short arm of rye chromosome 1R has been widely used to develop wheat cultivars through 1BL.1RS or 1AL.1RS translocation chromosomes ( Berzonsky et al, 1991 ; Rabinovich, 1998 ; Kumar et al, 2003 ; Landjeva et al, 2006 ). In fact, other rye chromosomes also contain elite genes that can be used for wheat improvement.…”

Section: Introductionmentioning

confidence: 99%

Physical Location of New PCR-Based Markers and Powdery Mildew Resistance Gene(s) on Rye (Secale cereale L.) Chromosome 4 Using 4R Dissection Lines

et al. 2017

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Rye (Secale cereale L.) 4R chromosome contains elite genes that are applicable for wheat (Triticum aestivum L.) cultivar improvement. PCR-based 4R-specific markers can benefit the detection of elite genes on 4R in wheat backgrounds. In this study, a new fluorescence in situ hybridization (FISH) map of the 4RKu chromosome of rye Kustro has been constructed. A set of 4RKu dissection lines was obtained and 301 new 4RKu-specific markers were developed using specific length amplified fragment sequencing (SLAF-seq) technology. These markers were combined with the 99 4RKu-specific markers previously developed, and were physically mapped to 4RKu chromosome using the new FISH map and the 4RKu dissection lines. A total of 338 of the 400 markers have been successfully mapped to six regions of 4RKu chromosome. Additionally, the powdery mildew resistance gene(s) on the 4RLKu arm was located to the segment between L.4 and L.8, the same region where 115 4RLKu-specific markers were mapped. The markers developed in this study can be used to identify a specific segment of 4R chromatin in wheat backgrounds, help construct a high-density physical map of 4R chromosome, and facilitate the utilization of elite genes on 4R chromosome in wheat breeding programs.

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1BL.1RS translocation in some Indian bread wheat genotypes and strategies for its use in future wheat breeding

Cited by 13 publications

References 25 publications

Wheat cytogenetics in the genomics era and its relevance to breeding

Wheat cytogenetics in the genomics era and its relevance to breeding

Recent Progress in Germplasm Evaluation and Gene Mapping to Enable Breeding of Drought-Tolerant Wheat

Physical Location of New PCR-Based Markers and Powdery Mildew Resistance Gene(s) on Rye (Secale cereale L.) Chromosome 4 Using 4R Dissection Lines

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