1H MR spectroscopy of normal and malignant human prostates in Vivo

Thomas, M. Albert; Narayan, Perinchery; Kurhanewicz, John; Jajodia, Prahalad; Weiner, Michael W.

doi:10.1016/0022-2364(90)90319-5

Cited by 33 publications

(34 citation statements)

References 16 publications

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“…Beginning in 1989, Narayan et al [14][15][16][17][18], developed the technique of performing human MRS studies using an endorectal coil. Initial feasibility studies with small surface coils were performed on Dunning rat prostate cancers grown on the flank, which led to the subsequent development of single-and double-tuned endorectal coils.…”

Section: Mrs Measurement Of Citratementioning

confidence: 99%

Citrate in the diagnosis of prostate cancer

1999

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Section: Mrs Measurement Of Citratementioning

confidence: 99%

Citrate in the diagnosis of prostate cancer

1999

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“…The 90" and 180' pulses were phase cycled in combination with the receiver for CYCLOPS to eliminate quadrature images and EXORCYCLE for proper refocussing of the 180" pulse yielding a degree of depth resolution (14). Partially relaxed spectra were obtained from 200 to 400 transients with a 15-to 20-ps in-plane 90° flip angle, 4K data points, a sweep width of 22000 Hz and a pulse repetition time of 5 s, yielding a total acquisition time of 1 Cancer tissues varying in size from 3 x 5 x 7 mm to 5 x 5 X 7 mm were pooled yielding sample weights from 0.20 to 0.94 g. The outer portion of the malignant nodule was not removed in order to assess tumor volume margins and extracapsular extension during pathologic examination. Prostatic adenocarcinoma was confirmed histologically using small sections from both ends of each tissue used for extraction.…”

Section: In Vivo 'H Spectroscopic Studiesmentioning

confidence: 99%

Citrate alterations in primary and metastatic human prostatic adenocarcinomas: ¹H magnetic resonance spectroscopy and biochemical study

Kurhanewicz

Dahiya

Macdonald

et al. 1993

Magnetic Resonance in Med

111

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The objectives of this study were to quantitatively verify the low levels of citrate previously observed in primary human prostatic adenocarcinomas and to determine whether citrate was further reduced in metastatic prostatic cancer. This was accomplished by comparison of citrate concentrations of DU 145 xenografts (a poorly differentiated human prostatic adenocarcinoma cell line grown in nude mice) with concentrations in primary human adenocarcinomas. Following in vivo 1H NMR studies of DU 145 xenografts, citrate concentrations of DU 145 xenografts and surgically removed primary prostatic adenocarcinoma tissue were determined by quantitative high resolution 1H NMR and enzymatic assay. The most significant findings of this study were that citrate concentrations in primary human adenocarcinomas (3.74 +/- 0.19 mumol/g wet weight) were significantly lower than those observed for normal and benign hyperplastic (BPH) prostatic tissues. Furthermore there was a further ten-fold reduction of citrate associated with DU 145 xenografts (0.31 +/- 0.028 mumole/g wet weight) compared with primary prostatic cancer. DU 145 xenografts also exhibited higher levels of uridine diphosphosugars and choline containing metabolites relative to primary prostatic adenocarcinomas. These findings support the hypothesis that citrate is low in primary prostatic cancer and further reduced in metastatic disease.

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“…The results showed that the water T,* in kidney was about 60 ms [about twice as long as in either muscle or liver). Proton line widths of about 5 Hz are therefore theoretically obtainable in the kidney. We were able to achieve water line widths in the kidney as low as 6 Hz for single scans, although the line widths of the metabolite signals were rather broader, possibly because of slight scan-to-scan variations due to motion.…”

Section: Discussionmentioning

confidence: 98%

Localized proton MR spectroscopy of the human kidney in vivo by means of short echo time STEAM sequences

Dixon¹,

Frahm²

1994

Magnetic Resonance in Med

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In order to obtain proton magnetic resonance spectra from the normal human kidney in vivo, we employed a STEAM sequence with delay times TE = 10 ms and TM = 30 ms. Signals are attenuated during STEAM sequences by J-coupling effects and by macroscopic movement of the sample. The combination of short echo times and respiratory triggering ensured that the kidney was stationary during the pulse sequence, and allowed us to detect strongly coupled resonances between 3 and 4.2 ppm. Analysis of spectra of extracts of bovine kidneys suggested that the renal MR-visible metabolites could include the osmolytes betaine, myo-inositol, and glycerophosphocholine. Four volunteers were subjected to overnight dehydration followed by rehydration, and we found that these signals increased significantly after dehydration, and decreased significantly 4 h after rehydration, thus supporting the assignment of the resonances as osmotically active metabolites.

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1H MR spectroscopy of normal and malignant human prostates in Vivo

Cited by 33 publications

References 16 publications

Citrate in the diagnosis of prostate cancer

Citrate in the diagnosis of prostate cancer

Citrate alterations in primary and metastatic human prostatic adenocarcinomas: ¹H magnetic resonance spectroscopy and biochemical study

Localized proton MR spectroscopy of the human kidney in vivo by means of short echo time STEAM sequences

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1H MR spectroscopy of normal and malignant human prostates in Vivo

Cited by 33 publications

References 16 publications

Citrate in the diagnosis of prostate cancer

Citrate in the diagnosis of prostate cancer

Citrate alterations in primary and metastatic human prostatic adenocarcinomas: 1H magnetic resonance spectroscopy and biochemical study

Localized proton MR spectroscopy of the human kidney in vivo by means of short echo time STEAM sequences

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Citrate alterations in primary and metastatic human prostatic adenocarcinomas: ¹H magnetic resonance spectroscopy and biochemical study