1H NMR metabolomic analysis of skin and blubber of bottlenose dolphins reveals a functional metabolic dichotomy

Misra, Biswapriya B.; Ruiz-Hernández, Ixchel M.; Hernández-Bolio, Gloria Ivonne; Hernández‐Núñez, Emanuel; Díaz-Gamboa, Raúl E.; Collí-Dulá, Reyna Cristina

doi:10.1016/j.cbd.2019.02.004

Cited by 12 publications

(9 citation statements)

References 57 publications

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“…This means that some tissues may primarily reflect individual diet components such as carbohydrates and lipids derived from one dietary source and proteins derived from another (76,77). By way of example, a study by Misra et al (2019) on bottlenose dolphins found that blubber tissue likely represents metabolic patterns linked to fatty acids and ketogenic amino acids related to fat synthesis and deposition within the tissue, whilst skin showed metabolites involved in gluconeogenic pathways pointing to active anabolic energy-generating metabolism. By extension, it is possible that the δ 13 C and δ 15 N values of SHHW blubber tissue may be drawn from a more direct energy pool, where lipids are immediately stored in the blubber, while secondary pathways may be involved in the growth of skin tissue.…”

Section: Resultsmentioning

confidence: 99%

Interpretation of southern hemisphere humpback whale diet via stable isotopes; implications of tissue-specific analysis

Eggebo

Groß

Nash

2022

Preprint

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Blubber and skin are commonly used tissues in stable isotope analysis for the purpose of investigating cetacean diet. Critical comparison of tissue-specific isotopic signals is, however, lacking resulting in uncertainty surrounding the representativeness and therefore utility of different tissues for accurate determination of recent foraging. This study used remotely biopsied blubber and skin tissues from southern hemisphere humpback whales for strategic comparison of δ 13 C and δ 15 N values. Samples were collected 2008-2018 as part of long-term monitoring under the Humpback Whale Sentinel Program. Blubber tissues were lipid-extracted prior to analysis, whilst mathematical lipid-correction was performed on skin samples. Isotopic values from paired blubber and skin samples from the same individuals were compared to assess whether tissues could be used interchangeably for isotope analysis and dietary interpretation. Significant differences were observed for both δ 13 C and δ 15 N, flagging previously undocumented methodological considerations, and the need for method validation and standardisation in application of these approaches. This study therefore advances methodological aspects of cetacean dietary analysis. This is of elevated importance in the context of rapidly changing ocean ecosystems.

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Section: Resultsmentioning

confidence: 99%

Interpretation of southern hemisphere humpback whale diet via stable isotopes; implications of tissue-specific analysis

Eggebo

Groß

Nash

2022

Preprint

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“…This DNM hinted that dolphins may enter a 'fat conservation' state more rapidly than expected and shows a tipping point is emerging in energy state transitions [123]. Managed or stranded cetaceans can be used as 'model species' to create a more comprehensive understanding of cetacean health and the physiological/ metabolic response to stressors as a way to create biomarkers to assess wild populations [123,[133][134][135][136][137][138][139]. For example, in managed whales, a correlation was found between lipidomics (i.e.…”

Section: Measuring Health In Cetaceans: Current and Novel Approachesmentioning

confidence: 99%

Climate change and cetacean health: impacts and future directions

Kebke

Samarra

Derous

2022

Phil. Trans. R. Soc. B

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Climate change directly impacts the foraging opportunities of cetaceans (e.g. lower prey availability), leads to habitat loss, and forces cetaceans to move to other feeding grounds. The rise in ocean temperature, low prey availability and loss of habitat can have severe consequences for cetacean survival, particularly those species that are already threatened or those with a limited habitat range. In addition, it is predicted that the concentration of contaminants in aquatic environments will increase owing to Arctic meltwater and increased rainfall events leading to higher rates of land-based runoff in downstream coastal areas. These persistent and mobile contaminants can bioaccumulate in the ecosystem, and lead to ecotoxicity with potentially severe consequences on the reproductive organs, immune system and metabolism of marine mammals. There is a need to measure and assess the cumulative impact of multiple stressors, given that climate change, habitat alteration, low prey availability and contaminants do not act in isolation. Human-caused perturbations to cetacean foraging abilities are becoming a pervasive and prevalent threat to many cetacean species on top of climate change-associated stressors. We need to move to a greater understanding of how multiple stressors impact the metabolism of cetaceans and ultimately their population trajectory. This article is part of the theme issue ‘Nurturing resilient marine ecosystems’.

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“…We can now characterize many thousands of genes, metabolites and proteins associated with phenotypic traits and this is key to the discovery of health biomarkers in human diseases (Abu-Asab et al, 2011;Hasin et al, 2017;Karczewski and Snyder, 2018;Peña-Bautista et al, 2018;Blencowe et al, 2019). To date, we still lack a detailed understanding of whole genome dynamics associated with changes in energy status in cetacean blubber and the consequences for cetacean cellular biology (Kershaw et al, 2018;Misra et al, 2019). However, recent attempts have been made to elucidate how metabolism of cetaceans is regulated by using an omics approach (Kershaw et al, 2018;Misra et al, 2019).…”

Section: Toward New Markers Of Health -Omics Approachmentioning

confidence: 99%

“…To date, we still lack a detailed understanding of whole genome dynamics associated with changes in energy status in cetacean blubber and the consequences for cetacean cellular biology (Kershaw et al, 2018;Misra et al, 2019). However, recent attempts have been made to elucidate how metabolism of cetaceans is regulated by using an omics approach (Kershaw et al, 2018;Misra et al, 2019). Proteomics data from full depth blubber subsamples from dead-stranded harbor porpoises identified 295 proteins mostly involved in cell function and metabolism (45%), immune response and inflammation (15%) and lipid metabolism (11%) (Kershaw et al, 2018).…”

Section: Toward New Markers Of Health -Omics Approachmentioning

confidence: 99%

“…Blubber metabolomics analysis of stranded bottlenose dolphins compared to skin biopsies indicated, unsurprisingly, a clear role of blubber in fatty acid metabolism and ketogenic amino acids. In addition both tissues were involved in urea cycle, nucleotide metabolism, amino acid metabolism, glutathione metabolism among others (Misra et al, 2019). Although these studies give us insight into the metabolome/proteome, it is hard to conclude the role of blubber in health status.…”

Section: Toward New Markers Of Health -Omics Approachmentioning

confidence: 99%

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Toward New Ecologically Relevant Markers of Health for Cetaceans

et al. 2020

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Multiple stressors caused by human-induced disturbances can affect the foraging opportunities of cetaceans, potentially depleting their energy stores, and ultimately impact survival and reproductive success. Currently, blubber thickness and lipid composition is used as measure of health and nutritional status in cetaceans. This assumes that blubber functions in the same manner as adipose tissue in terrestrial mammals. However, cetaceans have evolved to have thickened blubber which serves as thermoregulation, buoyancy and energy store. In addition, blubber is composed of several layers and regions that have different physiological functions. We currently lack a clear understanding of how blubber biology contributes to maintaining energy status in cetaceans and several studies show blubber thickness, and composition in some body regions, is an inappropriate measure of health. Before new markers of health can be identified, we need to understand how environmental stressors influence blubber biology and particularly unravel its complex signaling roles with other organs. Currently, we do not understand how changes in energy status drive changes in health in cetaceans, and eventually population dynamics. This review synthesizes recent developments in cetacean blubber biology to propose potential directions to develop novel cetacean health markers.

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1H NMR metabolomic analysis of skin and blubber of bottlenose dolphins reveals a functional metabolic dichotomy

Cited by 12 publications

References 57 publications

Interpretation of southern hemisphere humpback whale diet via stable isotopes; implications of tissue-specific analysis

Interpretation of southern hemisphere humpback whale diet via stable isotopes; implications of tissue-specific analysis

Climate change and cetacean health: impacts and future directions

Toward New Ecologically Relevant Markers of Health for Cetaceans

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