1H NMR-visible mobile lipid domains correlate with cytoplasmic lipid bodies in apoptotic T-lymphoblastoid cells

Vito, Massimo Di; Lenti, Luisa; Knijn, Arnold; Iorio, Egidio; D’Agostino, Federica; Molinari, Agnese; Calcabrini, Annarica; Stringaro, Annarita; Meschini, Stefania; Arancia, Giuseppe; Bozzi, Argante; Strom, Roberto; Podo, Franca

doi:10.1016/s1388-1981(00)00165-7

Cited by 56 publications

(46 citation statements)

References 41 publications

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“…An increase in the absorbance was likely due to increased lipid content within the cells such as the formation of lipid droplets [58]. Lipid droplets formed in T-lymphoblastoid (Jurkat) cells when apoptosis was induced by anthracyclins and other cancer drug controls [59]. The reason for the occurrence of the lipid droplets are still not clear, although the degradation of lipids, due to inhibition of the mitochondrial fatty acid β-oxidation pathway during apoptosis could be a possible reason [58,60].…”

Section: Early Stress Related Responses and Cytotoxicitymentioning

confidence: 99%

Cellular injury evidenced by impedance technology and infrared microspectroscopy

Roux

Prinsloo

Meyer

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Section: Early Stress Related Responses and Cytotoxicitymentioning

confidence: 99%

Cellular injury evidenced by impedance technology and infrared microspectroscopy

Roux

Prinsloo

Meyer

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…Drug-induced apoptosis studies have shown good correlations between apoptosis and nuclear magnetic resonance (NMR) visible mobile lipids and cytosolic lipid bodies in various human cancer cell lines: DU145 prostatic carcinoma cells (Milkevitch et al, 2005), Jurkat T-lymphoblast cells (Di Vito et al, 2001;Al-Saffar et al, 2002) and HuT 78 lymphoblastoid cells (Iorio et al, 2003), suggesting that MRS-detectable lipids accumulate in tumour cells undergoing apoptosis. In vivo studies of rodent tumour models have also shown that drug-induced apoptosis results in the accumulation of lipids and specifically polyunsaturated fatty acid (PUFA) resonances at ca.…”

Section: H Mrs; Magic Angle Spinning (Mas); Tumour; Astrocytoma; Apopmentioning

confidence: 99%

Taurine: a potential marker of apoptosis in gliomas

et al. 2009

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New cancer therapies are being developed that trigger tumour apoptosis and an in vivo method of apoptotic detection and early treatment response would be of great value. Magnetic resonance spectroscopy (MRS) can determine the tumour biochemical profile in vivo, and we have investigated whether a specific spectroscopic signature exists for apoptosis in human astrocytomas. Highresolution magic angle spinning (HRMAS) 1 H MRS provided detailed 1 H spectra of brain tumour biopsies for direct correlation with histopathology. Metabolites, mobile lipids and macromolecules were quantified from presaturation HRMAS 1 H spectra acquired from 41 biopsies of grades II (n ¼ 8), III (n ¼ 3) and IV (n ¼ 30) astrocytomas. Subsequently, TUNEL and H&E staining provided quantification of apoptosis, cell density and necrosis. Taurine was found to significantly correlate with apoptotic cell density (TUNEL) in both non-necrotic (R ¼ 0.727, P ¼ 0.003) and necrotic (R ¼ 0.626, P ¼ 0.0005) biopsies. However, the ca 2.8 p.p.m. polyunsaturated fatty acid peak, observed in other studies as a marker of apoptosis, correlated only in non-necrotic biopsies (R ¼ 0.705, Po0.005). We suggest that the taurine 1 H MRS signal in astrocytomas may be a robust apoptotic biomarker that is independent of tumour necrotic status.

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“…In order to remove proteins and other aqueous metabolites that could contribute to the CH 3 and CH 2 peaks in 1 H MR spectra (Kauppinen et al, 1993;Di Vito et al, 2001), further investigations of lipid metabolism were performed by monitoring lipid cell extracts at different time points. An example of the lipid phase 1 H MR spectrum of control Jurkat cell extracts is shown in Figure 3A.…”

Section: Experimental Therapeuticsmentioning

confidence: 99%

Apoptosis is associated with triacylglycerol accumulation in Jurkat T-cells

et al. 2002

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Magnetic resonance spectroscopy is increasingly used as a non-invasive method to investigate apoptosis. Apoptosis was induced in Jurkat T-cells by Fas mAb. 1 H magnetic resonance spectra of live cells showed an increase in methylene signal as well as methylene/methyl ratio of fatty acid side chains at 5 and 24 h following induction of apoptosis. To explain this observation, 1 H magnetic resonance spectra of cell extracts were investigated. These demonstrated a 70.0+7.0%, 114.0+8.0% and 90.0+5.0% increase in the concentration of triacylglycerols following 3, 5 and 7 h of Fas mAb treatment (P50.05). Confocal microscopy images of cells stained with the lipophilic dye Nile Red demonstrated the presence of lipid droplets in the cell cytoplasm. Quantification of the stained lipids by flow cytometry showed a good correlation with the magnetic resonance results (P50.05 at 3, 5 and 7 h). 31 P magnetic resonance spectra showed a drop in phosphatidylcholine content of apoptosing cells, indicating that alteration in phosphatidylcholine metabolism could be the source of triacylglycerol accumulation during apoptosis. In summary, apoptosis is associated with an early accumulation of mobile triacylglycerols mostly in the form of cytoplasmic lipid droplets. This is reflected in an increase in the methylene/methyl ratio which could be detected by magnetic resonance spectroscopy.

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1H NMR-visible mobile lipid domains correlate with cytoplasmic lipid bodies in apoptotic T-lymphoblastoid cells

Cited by 56 publications

References 41 publications

Cellular injury evidenced by impedance technology and infrared microspectroscopy

Cellular injury evidenced by impedance technology and infrared microspectroscopy

Taurine: a potential marker of apoptosis in gliomas

Apoptosis is associated with triacylglycerol accumulation in Jurkat T-cells

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