2. Characterization Of Lactic Acid Bacteria (LAB) Origin Sumatran Orangutan (Pongo abelii) In Zoo Bukittinggi Based On Analysis 16 S rRNA

Abstract

The purpose of this study was to characterize the bacterial 16S rRNA gene of Sumatran orangutan (Pongo abelii) Bukittinggi West Sumatera zoo. The sample used in this study are lactic acid bacteria of the Sumatran orangutan (Pongo abelii) derived from zoo Bukittinggi West Sumatra Indonesia. This study was an exploratory study that conducted at the Laboratory through several stages. The first stage was the isolation of LAB from faeces of Sumatran orangutans using MRS agar medium and then cultured in a liquid medium NB. The next stage was the isolation of total DNA, and then, the third stage was the amplification of the 16S rRNA gene and agarose gel electrophoresis. Then,in the fourth stage of determining the DNA sequence and analysis of DNA sequence homology. The final stage was the computational analysis of 16S rRNA gene Sumatran orangutan (Pongo abelii). The results showed that lactic acid bacteria (LAB) from the Sumatran orangutan (Pongo abelii) is close to lactic acid bacteria Lactobacillus helveticus strain IMAU50151 with the levelof similarity of 89%. It is possible that these bacteria is a new species or the species that LAB has not been reported in Genbank.

Identifikasi Lactobacillus sp pada Orangutan Sumatera (Pongo abelii) Liar Menggunakan KIT API 50 CHL di Stasiun Penelitian Suaq Belimbing Aceh Selatan

“…Hasil penelitian terhadap orangutan Kalimantan (Pongo pygmaeus) di Taman Safari Indonesia ditemukan 5,9x10 7 koloni bakteri asam laktat [5], sedangkan pada orangutan Sumatera (Pongo abelii) terdapat 7,2x10 6 koloni BAL, dengan ciri-ciri bakteri Gram positif, cocus dan basil [6]. Karakterisasi BAL pada orangutan sumetera yang ada di kebun binatang bukit tinggi dengan Gen 16S rRNA juga telah dilakukan oleh Hajar et al (2016) ; hasil penelitian ini menunjukan bahwa BAL pada orangutan Sumatera memiliki karakter yang dekat dengan BAL strain Lactobacillus helveticus strain IMAU50151 dengan tingkat homologi 89% [7]. Hasil penelitian Septiarini et al (2011) bahwa BAL dari feses orangutan (Pongo pygmaeus) mampu menghambat bakteri enteropatogen (Escherichia coli, Salmonella, dan Shigella) karena memiliki aktivitas antimikroba [8].…”

Weissella, a novel lactic acid bacteria isolated from wild Sumatran orangutans (Pongo abelii)

“…Studies on Sumatran orangutans, in captivity or nature reserves, found LAB colonies with Gram-positive cocci and basil bacteria. The characterization of LAB from Sumatran orangutans in high hill zoos with the 16S rRNA gene has also been carried out [11]. The results indicated that the LAB in Sumatran orangutans are closely related to the Lactobacillus helveticus IMAU50151 strain, with a homology of 89%.…”