2-Mercaptoethylamine, Radioprotector, inhibits the induction of the oxidative stress-inducible (soi) gene by paraquat in Escherichia coli

Kim, InGyu; Oh, Tae Jeong

doi:10.1006/phrs.2000.0708

Cited by 9 publications

(1 citation statement)

References 22 publications

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“…Several novel approaches are on the track to locate a potent radioprotector from plants. These include mimics of antioxidant enzymes [92] , nitroxides, melatonin [93] , growth factors [94] , gene therapy [95] and natural products [92,96] . The latter has several advantages since they are non-toxic with proven therapeutic benefits.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Research in Asia in the Period from 2000-2008

Hafidh¹,

Abdulamir²,

Bakar³

et al. 2009

American J. of Pharmacology and Toxicology

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Problem statement: Plant antioxidants are natural plant products that inhibit the adverse effects of the Reactive Oxygen Species (ROS) produced in plants. Thus, antioxidants enable plants to survive. Depending on this principle, human beings started using some plants' parts to cure from diseases many centuries ago, even before the discovery of microbes or disclosing the etiology of many human diseases. Shed light on the importance of diet and natural products that have proved of great health impact on human traditionally and scientifically, disclosing the central role of natural antioxidants in this matter. Moreover, this review tried to give an overview on the research has so far done in Asian countries particularly in the field of natural antioxidants during the period 2000-2008. Therefore, the aim of this review is to present a comprehensive index for the antioxidant property of herbs and plants that have been traditionally used as well as experimentally proved beneficial in these countries. Approach: We had compared Asian and other nation's diet in the popularity of the traditional medicine and the type of daily meal in respect to their antioxidants contents and valuable health promoting effects. Many literatures had been collected using online search engines and the main library of University Putra Malaysia. The gathered data had been arranged alphabetically in a table depending on the plant's scientific name, common name, the used part(s) and the family name. Results: The research had shown that there were seventy-six plants with proved antioxidant activity which had been well studied by Asian scientists in the chosen period of time. Conclusion: The antioxidant components of the natural products constitute the major source of human health promotion and maintenance. The nature is still the perfect source for health promotion and for the supplementation of safe drugs. Great attention is mandatory from all the scientists around the world to explore many underestimated plants with highly effective antioxidant activity.

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Section: Methodsmentioning

confidence: 99%

Antioxidant Research in Asia in the Period from 2000-2008

Hafidh¹,

Abdulamir²,

Bakar³

et al. 2009

American J. of Pharmacology and Toxicology

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Dose–response relationships and statistical performance of a battery of bacterial gene profiling assays

Dardenne

Nobels

Coen

et al. 2007

Appl Microbiol Biotechnol

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Because of increasing awareness and legislative demands, there is a demand for the development and use of biological assays for the assessment of the toxicity of chemicals, environmental samples. Recently, a growing number of bacterial reporter assays have been developed and implemented. Nevertheless, little data is published on the performance of these assays in terms of analytical parameters. We present results on a battery of 14 transgenic Escherichia coli strains carrying different promoter::reporter fusions. Growth characteristics and basal expression levels were modeled and fitted, data show that growth curves should be taken into account during test development. Our study shows that the induction profiles reflect the mode of action, e.g., paraquat clearly induces the soxRS operon. The sensitivity of the assay compares well to that of whole organism tests, e.g., fish and Daphnia for polar organics. Metal toxicity is detected less efficiently, e.g., cadmium is detected near the LC50 of carp, considered a relatively insensitive species towards cadmium. The assay variability ranges from 10 to 40% depending on the strain, comparable to that of other bioassays. The variability was shown to be determined by the intrinsic traits of the promoter-strain combination, not by operating conditions.

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Escherichia coli as a bioreporter in ecotoxicology

Robbens¹,

Dardenne

Devriese³

et al. 2010

Appl Microbiol Biotechnol

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Ecotoxicological assessment relies to a large extent on the information gathered with surrogate species and the extrapolation of test results across species and different levels of biological organisation. Bacteria have long been used as a bioreporter for genotoxic testing and general toxicity. Today, it is clear that bacteria have the potential for screening of other toxicological endpoints. Escherichia coli has been studied for years; in-depth knowledge of its biochemistry and genetics makes it the most proficient prokaryote for the development of new toxicological assays. Several assays have been designed with E. coli as a bioreporter, and the recent trend to develop novel, better advanced reporters makes bioreporter development one of the most dynamic in ecotoxicology. Based on in-depth knowledge of E. coli, new assays are being developed or existing ones redesigned, thanks to the availability of new reporter genes and new or improved substrates. The technological evolution towards easier and more sensitive detection of different gene products is another important aspect. Often, this requires the redesign of the bacterium to make it compatible with the novel measuring tests. Recent advances in surface chemistry and nanoelectronics open the perspective for advanced reporter based on novel measuring platforms and with an online potential. In this article, we will discuss the use of E. coli-based bioreporters in ecotoxicological applications as well as some innovative sensors awaited for the future.

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2-Mercaptoethylamine, Radioprotector, inhibits the induction of the oxidative stress-inducible (soi) gene by paraquat in Escherichia coli

Cited by 9 publications

References 22 publications

Antioxidant Research in Asia in the Period from 2000-2008

Antioxidant Research in Asia in the Period from 2000-2008

Dose–response relationships and statistical performance of a battery of bacterial gene profiling assays

Escherichia coli as a bioreporter in ecotoxicology

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