2012
DOI: 10.1007/s10895-012-1136-z
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2-NBDG Fluorescence Imaging of Hypermetabolic Circulating Tumor Cells in Mouse Xenograft model of Breast Cancer

Abstract: Objectives To determine use of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) as a tracer for detection of hypermetabolic circulating tumor cells (CTC) by fluorescence imaging. Procedures Human breast cancer cells were implanted in the mammary gland fat pad of athymic mice to establish orthotopic human breast cancer xenografts as a mouse model of circulating breast cancer cells. Near-infrared fluorescence imaging of the tumor-bearing mice injected with 2-DeoxyGlucosone 750 (2-DG 7… Show more

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Cited by 26 publications
(18 citation statements)
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“…(E) IVIS images of 2-DG-750 uptake in each individual mouse. Excretory 2-DG-750 fluorescence signal was also visualized in the lower abdominal region near the bladder for several mice, as described by others (44); however, only the fluorescence signal at the primary site of the mammary xenograft tumors was considered for our analysis of total flux glucose uptake.…”
Section: Figurementioning
confidence: 99%
“…(E) IVIS images of 2-DG-750 uptake in each individual mouse. Excretory 2-DG-750 fluorescence signal was also visualized in the lower abdominal region near the bladder for several mice, as described by others (44); however, only the fluorescence signal at the primary site of the mammary xenograft tumors was considered for our analysis of total flux glucose uptake.…”
Section: Figurementioning
confidence: 99%
“…3D). the circulating tumor cells can be detected in the bloodstream of patients using microchip technology, immunomagnetic nanoscreening and 2-nBDg fluorescence imaging (182)(183)(184). In order to survive in the circulation, tumor cells recruit platelets which, in turn, form a coat to protect them from the innate immune system.…”
Section: Activation Of Invasion and Metastasismentioning
confidence: 99%
“…Exogenous indicators, however, have been used when the goal is to quantify specific substrate uptake (eg, glucose) and metabolic output (mitochondrial metabolism) in a direct and explicit manner. The fluorescent analog to fluoro‐deoxy‐glucose (FDG), 2‐[ N ‐(7‐nitrobenz‐2‐oxa‐1, 3‐diazol‐4‐yl) amino]‐2‐deoxy‐ d ‐glucose (2‐NBDG) has been extensively validated in cell and animal model studies to report on glucose uptake analogous to widely accepted PET imaging . A key indicator of mitochondrial metabolism is the mitochondrial membrane potential (MMP) maintained by the electron transport chain .…”
Section: Introductionmentioning
confidence: 99%