20th anniversary of axial capacitively coupled contactless conductivity detection in capillary electrophoresis

Kubáň, Pavel; Hauser, Peter C.

doi:10.1016/j.trac.2018.03.007

Cited by 118 publications

(60 citation statements)

References 157 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The moderate detection sensitivity typical for C 4 D when coupled with CE (generally in the range of 0.1-1 μM) is not always a limitation as sufficiently high concentrations of target analytes are often present when facing with aforementioned analytical issues in developing countries. An overview of the 20year development of CE-C 4 D, covering details on working principles and applications, can be found in a recent review by Hauser and Kubáň [6]. Over 15 years, we have designed and developed various CE-C 4 D versions based on feedbacks of the users, aiming at better adaptation to screening analysis for the population.…”

Section: Introductionmentioning

confidence: 99%

Low‐cost and versatile analytical tool with purpose‐made capillary electrophoresis coupled to contactless conductivity detection: Application to antibiotics quality control in Vietnam

Hauser

Thị

et al. 2020

Electrophoresis

Self Cite

View full text Add to dashboard Cite

In this study, the development of our purpose‐made capacitively coupled contactless conductivity detection (C4D) for CE is reported. These systems have been employed as a simple, versatile, and cost‐effective analytical tool. CE‐C4D devices, whose principle is based on the control of the ion movements under an electrical field, can be constructed even with a modest financial budget and limited infrastructure. A featured application was developed for quality control of antimicrobial drugs using CE‐C4D, with most recent work on determination of aminoglycoside and glycopeptide antibiotics being communicated. For aminoglycosides, the development of CE‐C4D methods was adapted to two categories. The first one includes drugs (liquid or powder form) for intravenous injection, containing either amikacin, streptomycin, kanamycin A, or kanamycin B. The second one covers drugs for eye drops (liquid or ointment form), containing either neomycin, tobramycin, or polymyxin. The CE‐C4D method development was also made for determination of some popular glycopeptide antibiotics in Vietnam, including vancomycin and teicoplanin. The best detection limit achieved using the developed CE‐C4D methods was 0.5 mg/L. Good agreement between results from CE‐C4D and the confirmation method (HPLC‐ Photometric Diode Array ) was achieved, with their result deviations less than 8% and 13% for aminoglycoside and glycopeptide antibiotics, respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Low‐cost and versatile analytical tool with purpose‐made capillary electrophoresis coupled to contactless conductivity detection: Application to antibiotics quality control in Vietnam

Hauser

Thị

et al. 2020

Electrophoresis

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to amperometric modes, capacitively coupled contactless conductivity detection (C 4 D) has gained increasingly popularity to monitor separations in microscale due to its advantages associated to the electrical insulation, simplicity and absence of interferences from the electrical field applied to electrophoresis . In 2003, Carvalho and co‐workers described a pioneering report showing the use of C 4 D to monitor separations of monosaccharides and disaccharides in conventional CE using a strongly alkali medium .…”

Section: Introductionmentioning

confidence: 99%

Separation of carbohydrates on electrophoresis microchips with controlled electrolysis

et al. 2019

View full text Add to dashboard Cite

This study reports the separation of fructose, galactose, glucose, lactose and sucrose on glass microchip electrophoresis (ME) devices using a microfluidic platform adapted with external reservoirs for controlling the electrolysis phenomenon. The connections between external reservoirs and microfluidic platform were performed by saline bridges created using silicone tubing filled with BGE. The separation conditions were optimized and the best results were achieved using a BGE containing 75 mmol/L NaOH and 15 mmol/L trisodium phosphate. Electrophoretic separations were monitored using a capacitively coupled contactless conductivity detection system. The controlled electrolysis has successfully allowed the application of a higher voltage on the separation channel promoting the baseline separation of five carbohydrates within 180 s with great run‐to‐run repeatability (RSD < 1%). The achieved efficiencies ranged from 45 000 ± 6000 to 70 000 ± 3000 plates/m demonstrating a performance better than ME devices without controlled electrolysis. The proposed system offered good linearity from 1 to 10 mmol/L and LODs between 150 and 740 μmol/L. The use of external tubes for controlling the electrolysis phenomenon on ME devices has solved common problems associated to run‐to‐run repeatability and analytical reliability required for routine and quantitative analysis.

show abstract

“…Moreover, the use of UV spectrometry, especially in capillaries with id 25 μm or less, thus does not enable attainment of sufficient sensitivity for pharmacokinetic studies on living organisms. It has been found to be much better to employ contactless conductivity detection (C 4 D) , which can be used to detect ketamine in its native form without the necessity of demanding derivatisation, substantially simplifying the laboratory treatment of small amounts of clinical samples. In addition, the sensitivity of C 4 D in CE does not decrease with decreasing id of the capillary; using a suitable combination of the frequency of the exciting carrier signal together with the optimization of BGE conductivity, enables attaining an LOD at the submicromolar concentration level in capillaries with id 25 μm or less .…”

Section: Introductionmentioning

confidence: 99%

Rapid electrophoretic monitoring of the anaesthetic ketamine and its metabolite norketamine in rat blood using a contactless conductivity detector to study the pharmacokinetics

Tůma

Sommerová

Vaculín

2019

J of Separation Science

View full text Add to dashboard Cite

A method of capillary electrophoresis with contactless conductivity detection has been developed for non-enantioselective monitoring the anaesthetic ketamine and its main metabolite norketamine. The separation is performed in a 15 μm capillary with an overall length of 31.5 cm and length to detector of 18 cm; inner surface of the capillary is covered with a commercial coating solution to reduce the electroosmotic flow. In an optimised background electrolyte with composition 2 M acetic acid + 1% v/v coating solution under application of a high voltage of 30 kV, the migration time is 97.1 s for ketamine and 95.8 s for norketamine, with an electrophoretic resolution of 1.2. The attained detection limit was 83 ng/mL (0.3 μmol/L) for ketamine and 75 ng/mL (0.3 μmol/L) for norketamine; the number of theoretic plates for separation of an equimolar model mixture with a concentration of 2 μg/mL was 683 500 plates/m for ketamine and 695 400 plates/m for norketamine. Laboratory preparation of rat blood plasma is based on mixing 10 μL of plasma with 30 μL of acidified acetonitrile, followed by centrifugation. A pharmacokinetic study demonstrated an exponential decrease in the plasma concentration of ketamine after intravenous application and much slower kinetics for intraperitoneal application. K E Y W O R D Sacetonitrile stacking, capillary electrophoresis, contactless conductivity detection, ketamine, pharmacokinetics 2062

show abstract

20th anniversary of axial capacitively coupled contactless conductivity detection in capillary electrophoresis

Cited by 118 publications

References 157 publications

Low‐cost and versatile analytical tool with purpose‐made capillary electrophoresis coupled to contactless conductivity detection: Application to antibiotics quality control in Vietnam

Low‐cost and versatile analytical tool with purpose‐made capillary electrophoresis coupled to contactless conductivity detection: Application to antibiotics quality control in Vietnam

Separation of carbohydrates on electrophoresis microchips with controlled electrolysis

Rapid electrophoretic monitoring of the anaesthetic ketamine and its metabolite norketamine in rat blood using a contactless conductivity detector to study the pharmacokinetics

Contact Info

Product

Resources

About