218 The Regulation of the Intestinal Mucin MUC2 Expression By Short Chain Fatty Acids: Implications for Epithelial Protection

Paassen, Nanda Burger‐van; Vincent, Audrey; Puiman, Patrycja; Sluis, Maria van der; Bouma, J.; Boehm, G; Goudoever, Hans van; Seuningen, Isabelle Van; Renes, Ingrid B.

doi:10.1016/s0016-5085(09)60189-x

Cited by 12 publications

(12 citation statements)

References 20 publications

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“…This is in accordance with a previous report showing the ability of F. prausnitzii to produce butyric acid or other SCFAs (Machiels et al, 2014). Upregulation of SCFAs, especially butyric acid, can reduce the pH of the intestinal lumen and trigger a number of signaling effects including the regulation of mucin synthesis and secretion (Paassen et al, 2009), further reinforcing the intestinal barrier (Wong et al, 2006). An earlier report provided direct in vivo evidence that F. prausnitzii was able to utilize acetate produced by B. thetaiotaomicron and that this symbiotic relationship exerted far-reaching and beneficial influences on the regulation of the bowel mucus barrier (Wrzosek et al, 2013).…”

Section: Discussionsupporting

confidence: 93%

Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice

Zhai

Feng

et al. 2020

Front. Microbiol.

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Oral exposure to the heavy metal lead (Pb) causes various dysfunctions in animals. However, the influence of gut bacteria on Pb absorption, bioaccumulation, and excretion is largely unknown. In this study, we use a mouse model to investigate the relationship between gut microbiota, Pb-intolerant intestinal microbes and Pb toxicity. First, mice were treated with a broad-spectrum antibiotic cocktail to deplete their gut microbiota, and were then acutely and orally exposed to Pb at 1304 mg/kg for 3 days. Compared to the control mice, antibiotic-treated mice had increased Pb concentrations in the blood and primary organs and decreased Pb fecal concentrations, suggesting that gut microbiota limited the Pb burden that developed from acute oral Pb exposure. Next, three Pb-intolerant gut microbes, Akkermansia muciniphila, Faecalibacterium prausnitzii, and Oscillibacter ruminantium, were orally administered to mice, and their effects against Pb toxicity were evaluated. F. prausnitzii treatment significantly promoted the fecal Pb excretion and reduced Pb concentrations in blood (from 152.70 ± 25.62 µg/dL to 92.20 ± 24.33 µg/dL) and primary tissues. Supplementation with O. ruminantium significantly decreased Pb concentrations in blood (from 152.70 ± 25.62 µg/dL to 104.60 ± 29.85 µg/dL) and kidney (from 7.30 ± 1.08 µg/g to 5.64 ± 0.79 µg/g). Treatment with F. prausnitzii and O. ruminantium also upregulated tight junction (TJ) protein expression and the production of short-chain fatty acids by colonic microbiota, and showed protective effects against liver and kidney toxicity. These results indicate the potential for reducing Pb toxicity by the modulation of gut microbiota.

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Section: Discussionsupporting

confidence: 93%

Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice

Zhai

Feng

et al. 2020

Front. Microbiol.

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“…With low concentrations of SCFAs, there was a significant increase in MUC2 expression while higher concentrations decreased production in mice. 30,31 Similarly, ex vivo stimulation of colon tissue with 0.05-1mM butyrate induced MUC2 synthesis, whereas higher concentrations returned MUC2 synthesis to basal levels. 32 Even though the molecular mechanisms behind this increase in MUC2 expression by SCFAs are not well understood, butyrate was shown to induce MUC2 transcription through AP-1 binding and acetylation of histones at the MUC2 promoter.…”

Section: Mucus and Microbiotamentioning

confidence: 99%

“…32 Even though the molecular mechanisms behind this increase in MUC2 expression by SCFAs are not well understood, butyrate was shown to induce MUC2 transcription through AP-1 binding and acetylation of histones at the MUC2 promoter. 31 …”

Section: Mucus and Microbiotamentioning

confidence: 99%

Roles and regulation of the mucus barrier in the gut

2015

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“…More specifically, this regulation might act as a mechanism to facilitate absorption after a meal [79]. SCFAs are essential for the maintenance of mucosal immunity by stimulating the expression of mucin (MUC2) synthesis and its secretion by goblet-like colon cells [80]. They also stimulate antimicrobial peptides (AMPs), so that they exert an innate defence against pathogens and modulate tight junction (TJs) formation and their permeability potentially through activation of Adenosine monophosphate-activated protein kinase (AMPK) [81,82].…”

Section: Gut Microbiota Metabolitesmentioning

confidence: 99%

Gut Microbiota as Important Mediator Between Diet and DNA Methylation and Histone Modifications in the Host

et al. 2020

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The human gut microbiota is a complex ecosystem consisting of trillions of microorganisms that inhabit symbiotically on and in the human intestine. They carry out, through the production of a series of metabolites, many important metabolic functions that complement the activity of mammalian enzymes and play an essential role in host digestion. Interindividual variability of microbiota structure, and consequently of the expression of its genes (microbiome), was largely ascribed to the nutritional regime. Diet influences microbiota composition and function with short- and long-term effects. In spite of the vast literature, molecular mechanisms underlying these effects still remain elusive. In this review, we summarized the current evidence on the role exerted by gut microbiota and, more specifically, by its metabolites in the establishment of the host epigenome. The interest in this topic stems from the fact that, by modulating DNA methylation and histone modifications, the gut microbiota does affect the cell activities of the hosting organism.

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218 The Regulation of the Intestinal Mucin MUC2 Expression By Short Chain Fatty Acids: Implications for Epithelial Protection

Cited by 12 publications

References 20 publications

Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice

Oral Supplementation of Lead-Intolerant Intestinal Microbes Protects Against Lead (Pb) Toxicity in Mice

Roles and regulation of the mucus barrier in the gut

Gut Microbiota as Important Mediator Between Diet and DNA Methylation and Histone Modifications in the Host

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