[28] Ultrastructural studies of cell—collagen interactions

Grinnell, Frederick; Bennett, Marylyn Hoy

doi:10.1016/0076-6879(82)82085-5

Cited by 37 publications

(17 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…15,[27][28][29][30][31][32][33][34] Collagen is a natural extracellular matrix component of many types of tissues, and its nano-fibrous architecture has long been noticed to play a role in cell adhesion, growth, and differentiated function in tissue cultures. 16,35,36 To mimic the nano-fibrous architecture and overcome the concerns of disease transmission and immunogenicity associated with collagen, biodegradable polymers have been successfully processed into nano-fibrous matrices in our laboratory recently. 3 In this report, we have successfully demonstrated how to create a variety of macroporous architectures in the nano-fibrous matrices, aimed to improve spatial cellular distribution, mass transfer, and new tissue organization.…”

Section: Discussionmentioning

confidence: 99%

Synthetic nano-fibrillar extracellular matrices with predesigned macroporous architectures

Zhang

Peter

2000

J. Biomed. Mater. Res.

251

166

View full text Add to dashboard Cite

Scaffolding plays a pivotal role in tissue engineering. To mimic the architecture of a natural extracellular matrix component-collagen, nona-fibrous matrices have been created with synthetic biodegradable polymers in our laboratory using a phase-separation technique. To improve the cell seeding, distribution, mass transport, and new tissue organization, three-dimensional macroporous architectures are built in the nano-fibrous matrices. Water-soluble porogen materials are first fabricated into three-dimensional negative replicas of the desired macroporous architectures. Polymer solutions are then cast over the porogen assemblies in a mold, and are thermally phase-separated to form nano-fibrous matrices. The porogen materials are leached out with water to finally form the synthetic nano-fibrous extracellular matrices with predesigned macroporous architectures. In this way, synthetic polymer matrices are created with architectural features at several levels, including the anatomical shape of the matrix, macroporous elements (100 microm to millimeters), interfiber distance (microns), and the diameter of the fibers (50-500 nm). These scaffolding materials circumvent the concerns of pathogen transmission and immuno-rejection associated with natural collagen. With the flexibility in the design of chemical structure, molecular weight, architecture, degradation rate, and mechanical properties, these novel synthetic matrices may serve as superior scaffolding for tissue engineering.

show abstract

Section: Discussionmentioning

confidence: 99%

Synthetic nano-fibrillar extracellular matrices with predesigned macroporous architectures

Zhang

Peter

2000

J. Biomed. Mater. Res.

251

166

View full text Add to dashboard Cite

show abstract

“…These fibers resemble the fibrillar structure of collagen in both morphology and size, along with many other ECM components. Various synthetic nanofibers have been found to enhance cell-matrix interactions, resulting in improved cell adhesion, proliferation and differentiation [33][34][35][36][37][38][39][40][41]. Self-assembly, electrospinning and phase separation are the three techniques currently used for the fabrication of macroscopically sized scaffolds featuring what we define as 'nanofibrous (NF) morphology.'…”

Section: Tissue Engineering Scaffold Designmentioning

confidence: 99%

Nanostructured Injectable Cell Microcarriers for Tissue Regeneration

Zhang

Eyster

Peter

2016

Nanomedicine (Lond.)

View full text Add to dashboard Cite

Biodegradable polymer microspheres have emerged as cell carriers for the regeneration and repair of irregularly shaped tissue defects due to their injectability, controllable biodegradability and capacity for drug incorporation and release. Notably, recent advances in nanotechnology allowed the manipulation of the physical and chemical properties of the microspheres at the nanoscale, creating nanostructured microspheres mimicking the composition and/or structure of natural extracellular matrix. These nanostructured microspheres, including nanocomposite microspheres and nanofibrous microspheres, have been employed as cell carriers for tissue regeneration. They enhance cell attachment and proliferation, promote positive cell-carrier interactions and facilitate stem cell differentiation for target tissue regeneration. This review highlights the recent advances in nanostructured microspheres that are employed as injectable, biomimetic and cell-instructive cell carriers.

show abstract

“…It is reported that nanoscale collagen (a major component of the ECM) fiber structures can boost the interaction between cell and the surrounding matrix. 18 In preliminary studies, the mimicking of ECM approach has been used to prepare nanomaterials for stem cell research. Those materials turned out to be meaningful for the cellular expansion of self-renewing pluripotent ESCs.…”

Section: Differentiation Of Stem Cellsmentioning

confidence: 99%

The Role of Nanotechnology in Induced Pluripotent and Embryonic Stem Cells Research

Chen¹,

Qiu²,

Li³

2014

j biomed nanotechnol

View full text Add to dashboard Cite

This paper reviews the recent studies on development of nanotechnology in the field of induced pluripotent and embryonic stem cells. Stem cell therapy is a promising therapy that can improve the quality of life for patients with refractory diseases. However, this option is limited by the scarcity of tissues, ethical problem, and tumorigenicity. Nanotechnology is another promising therapy that can be used to mimic the extracellular matrix, label the implanted cells, and also can be applied in the tissue engineering. In this review, we briefly introduce implementation of nanotechnology in induced pluripotent and embryonic stem cells research. Finally, the potential application of nanotechnology in tissue engineering and regenerative medicine is also discussed.

show abstract

[28] Ultrastructural studies of cell—collagen interactions

Cited by 37 publications

References 7 publications

Synthetic nano-fibrillar extracellular matrices with predesigned macroporous architectures

Synthetic nano-fibrillar extracellular matrices with predesigned macroporous architectures

Nanostructured Injectable Cell Microcarriers for Tissue Regeneration

The Role of Nanotechnology in Induced Pluripotent and Embryonic Stem Cells Research

Contact Info

Product

Resources

About