[29] Long-term alkaloid production by immobilized cells of Claviceps purpurea

Kopp, Bettina

doi:10.1016/s0076-6879(87)36031-8

Cited by 9 publications

(3 citation statements)

References 10 publications

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“…However, high inoculum sizes might not necessarily give higher enzyme yield or cell growth. In addition, greater inoculum size can lead to an impaired nutrient supply and thereby decreasing bacterial growth and production capacity (Kopp 1987). Previous studies have identified different optimum inoculum sizes 1% (v/v; Kambourova et al 2003), 3% (v/v; Gupta et al 2007), 6% Rahman et al 2005b) and 10% (v/v;Jasvir et al 1999) for lipase production of different enzymes.…”

Section: Log Cfu/mlmentioning

confidence: 99%

A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical factor studies

Masomian

Rahman

Salleh

et al. 2010

World J Microbiol Biotechnol

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A newly isolated thermophilic bacterium, Aneurinibacillus thermoaerophilus strain HZ, from a hot spring recreational area (Sungai Kelah, Malaysia), showed an extracellular lipase activity. It was identified based on 16S rRNA sequencing, where phylogenetic analysis revealed its homology to Aneurinibacillus thermoaerophilus. The strain produced a lipase that was stable in various organic solvents such as dimethyl sulfoxide, toluene, p-xylene, and hexane. In order to increase lipase production, optimization of physical factors which affected the growth and lipase production was studied. The optimal growth was obtained at 50°C and pH 8.0; while the maximal lipase production was achieved in the logarithmic decline phase at 60°C and pH 7.5 with 7% starting inoculum and 150 rev/min shaking rate for 48 h incubation.

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Section: Log Cfu/mlmentioning

confidence: 99%

A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical factor studies

Masomian

Rahman

Salleh

et al. 2010

World J Microbiol Biotechnol

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“…Wet cells were harvested by centrifuging the 18 h culture broth at 10,000 rpm for 10 min. The cells were resuspended in presterilized alginate solution (2-5% w/v) to make a 20% (w/v) cell suspension (9). The cell slurry was extruded dropwise through micropipetters (5 ml, 1 ml syringe) into sterile CaC12 or SrCl2 solution (0.1-0.3 M).…”

Section: Methodsmentioning

confidence: 99%

Optimum conditions for the production of lipase by alginate-immobilized bacteria.

Zakaria

Razak

Ampon

et al. 1992

J. Gen. Appl. Microbiol.

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“…The immobilization of C. purpurea has been shown to have a distinct influence on alkaloid production (see Kopp 1987;Kopp andRehm 1983, 1984;Hartmeier and Schmitz 1987). The effects of limitation seem important, as well as the consistency of the biocatalyst.…”

Section: Introductionmentioning

confidence: 99%

Influence of inorganic phosphate and immobilization on Claviceps purpurea

Lohmeyer

Dierkes

Rehm

1990

Appl Microbiol Biotechnol

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The influence of inorganic phosphate and immobilization on cells of Claviceps purpurea strain 1029/N5 producing ergot peptides in shake culture was examined. Immobilization in Ca-alginate beads resulted in a marked reduction of some metabolic activities, i.e. the periods of alkaloid formation and cell growth were prolonged. High concentrations of inorganic phosphate (1 g/l KHzPO4) could reduce or stop alkaloid formation both by free and immobilized cells at any time during fermentation. The optimum phosphate concentration for alkaloid production by immobilized cells (about 0.5 mM) was a quarter of that required by free cells. This optimum shift was attributed to (i) the diminished phosphate demand of immobilized cells, due to their reduced metabolic activities, and (ii) the phosphate-dependent morphological behaviour of the biocatalyst. The observed decrease in alkaloid concentrations during later periods of the fermentation supported the idea of alkaloid-degradative enzymes, activated by high phosphate concentrations. Immobilization showed an advantageous influence on this undesirable effect.

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[29] Long-term alkaloid production by immobilized cells of Claviceps purpurea

Cited by 9 publications

References 10 publications

A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical factor studies

A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical factor studies

Optimum conditions for the production of lipase by alginate-immobilized bacteria.

Influence of inorganic phosphate and immobilization on Claviceps purpurea

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