3-D Super-Resolution Ultrasound Imaging for Monitoring Early Changes in Breast Cancer after Treatment with a Vascular-Disrupting Agent

Oezdemir, Ipek; Li, Junjie; Song, Jinmei; Hoyt, Kenneth

doi:10.1109/ius52206.2021.9593426

Cited by 2 publications

(4 citation statements)

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“…For instance, CEUS imaging with time-intensity curve analyses were employed to detect a significant early decrease of tumor vascularization and perfusion in various preclinical tumor models treated with VDAs. 24,25,36,37 This is analogous to the SRUS image derived TMC curve measurements established for our study. Photoacoustic imaging is another noninvasive modality, which functions by using both optical and ultrasound waves to generate images of the tumor vasculature.…”

Section: Discussionsupporting

confidence: 59%

“…Previous research has also investigated the use of various ultrasound techniques for monitoring tumor response to VDA treatment. For instance, CEUS imaging with time‐intensity curve analyses were employed to detect a significant early decrease of tumor vascularization and perfusion in various preclinical tumor models treated with VDAs 24,25,36,37 . This is analogous to the SRUS image derived TMC curve measurements established for our study.…”

Section: Discussionmentioning

confidence: 91%

“…After ultrasound imaging and direct pressure measures at 4 or 24 hours (6 and 8, control and treated animals, respectively, for each), tomato lectin labeled with Texas red (Vector Laboratories, Burlingame, CA) was injected through the tail vein. 25 Animals were then humanely euthanized by cervical dislocation and excised tumor tissue samples were fixed in formalin and cut into 10 μm sections. Whole tissue cross-sections were optically scanned and digitized at Â20 magnification (Axio Observer 7, Carl Zeiss Microscopy, White Plains, NY).…”

Section: Histological Analysismentioning

confidence: 99%

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Super‐Resolution Ultrasound Imaging for Monitoring the Therapeutic Efficacy of a Vascular Disrupting Agent in an Animal Model of Breast Cancer

Hoyt

2024

J of Ultrasound Medicine

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ObjectiveEvaluate the use of super‐resolution ultrasound (SRUS) imaging for the early detection of tumor response to treatment using a vascular‐disrupting agent (VDA).MethodsA population of 28 female nude athymic mice (Charles River Laboratories) were implanted with human breast cancer cells (MDA‐MB‐231, ATCC) in the mammary fat pad and allowed to grow. Ultrasound imaging was performed using a Vevo 3100 scanner (FUJIFILM VisualSonics Inc) equipped with the MX250 linear array transducer immediately before and after receiving bolus injections of a microbubble (MB) contrast agent (Definity, Lantheus Medical Imaging) via the tail vein. Following baseline ultrasound imaging, VDA drug (combretastatin A4 phosphate, CA4P, Sigma Aldrich) or control saline was injected via the placed catheter. After 4 or 24 hours, repeat ultrasound imaging along the same tumor cross‐section occurred. Direct intratumoral pressure measurements were obtained using a calibrated sensor. All raw ultrasound data were saved for offline processing and SRUS image reconstruction using custom MATLAB software (MathWorks Inc). From a region encompassing the tumor space and the entire postprocessed ultrasound image sequence, time MB count (TMC) curves were generated in addition to traditional SRUS maps reflecting MB enumeration at each pixel location. Peak enhancement (PE) and wash‐in rate (WIR) were extracted from these TMC curves. At termination, intratumoral microvessel density (MVD) was quantified using tomato lectin labeling of patent blood vessels.ResultsSRUS images exhibited a clear difference between control and treated tumors. While there was no difference in any group parameters at baseline (0 hour, P > .09), both SRUS‐derived PE and WIR measurements in tumors treated with VDA exhibited significant decreases by 4 (P = .03 and P = .05, respectively) and 24 hours (P = .02 and P = .01, respectively), but not in control group tumors (P > .22). Similarly, SRUS derived microvascular maps were not different at baseline (P = .81), but measures of vessel density were lower in treated tumors at both 4 and 24 hours (P < .04). An inverse relationship between intratumoral pressure and both PE and WIR parameters were found in control tumors (R2 > .09, P < .03).ConclusionSRUS imaging is a new modality for assessing tumor response to treatment using a VDA.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 91%

Section: Histological Analysismentioning

confidence: 99%

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Super‐Resolution Ultrasound Imaging for Monitoring the Therapeutic Efficacy of a Vascular Disrupting Agent in an Animal Model of Breast Cancer

Hoyt

2024

J of Ultrasound Medicine

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“…Opacic et al showed that US SRI could provide a number of clinical parameters such as velocities and directions of movement in individual vessels which can differentiate tumors with different vascular phenotypes, and it may provide opportunities for functional characterization of tumors and the assessment of therapy response. To assess any early changes in breast cancer following vascular disrupting agent (VDA) treatment, a study using 3-dimensional (3-D) super-resolution ultrasound (SR-US) imaging to observe MVD in breast lesions showed that the MVD of each tumor volume was significantly reduced at 24 hours after VDA treatment (41), indicating the feasibility of super-resolution technique for disease diagnosis via calculating micro-vessel density and microvascular flow rate in masses. However, there is no previous study reported the application of US SRI on human thyroid nodule to differentiate the benign and malignancy.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound super-resolution imaging for the differential diagnosis of thyroid nodules: A pilot study

Zhang

Lei

et al. 2022

Front. Oncol.

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ObjectiveUltrasound imaging provides a fast and safe examination of thyroid nodules. Recently, the introduction of super-resolution imaging technique shows the capability of breaking the Ultrasound diffraction limit in imaging the micro-vessels. The aim of this study was to evaluate its feasibility and value for the differentiation of thyroid nodules.MethodsIn this study, B-mode, contrast-enhanced ultrasound, and color Doppler flow imaging examinations were performed on thyroid nodules in 24 patients. Super-resolution imaging was performed to visualize the microvasculature with finer details. Microvascular flow rate (MFR) and micro-vessel density (MVD) within thyroid nodules were computed. The MFR and MVD were used to differentiate the benign and malignant thyroid nodules with pathological results as a gold standard.ResultsSuper-resolution imaging (SRI) technique can be successfully applied on human thyroid nodules to visualize the microvasculature with finer details and obtain the useful clinical information MVD and MFR to help differential diagnosis. The results suggested that the mean value of the MFR within benign thyroid nodule was 16.76 ± 6.82 mm/s whereas that within malignant thyroid was 9.86 ± 4.54 mm/s. The mean value of the MVD within benign thyroid was 0.78 while the value for malignant thyroid region was 0.59. MFR and MVD within the benign thyroid nodules were significantly higher than those within the malignant thyroid nodules respectively (p < 0.01).ConclusionsThis study demonstrates the feasibility of ultrasound super-resolution imaging to show micro-vessels of human thyroid nodules via a clinical ultrasound platform. The important imaging markers, such as MVD and MFR, can be derived from SRI to provide more useful clinical information. It has the potential to be a new tool for aiding differential diagnosis of thyroid nodules.

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3-D Super-Resolution Ultrasound Imaging for Monitoring Early Changes in Breast Cancer after Treatment with a Vascular-Disrupting Agent

Cited by 2 publications

References 25 publications

Super‐Resolution Ultrasound Imaging for Monitoring the Therapeutic Efficacy of a Vascular Disrupting Agent in an Animal Model of Breast Cancer

Super‐Resolution Ultrasound Imaging for Monitoring the Therapeutic Efficacy of a Vascular Disrupting Agent in an Animal Model of Breast Cancer

Ultrasound super-resolution imaging for the differential diagnosis of thyroid nodules: A pilot study

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