3-Deoxysappanchalcone Promotes Proliferation of Human Hair Follicle Dermal Papilla Cells and Hair Growth in C57BL/6 Mice by Modulating WNT/β-Catenin and STAT Signaling

Kim, Young Eun; Choi, Hyung Chul; Lee, In Chul; Yuk, Dong Yeon; Lee, Hyosung; Choi, Bu Young

doi:10.4062/biomolther.2016.183

Cited by 32 publications

(24 citation statements)

References 39 publications

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“…Accumulation of β-catenin in the cytosol leads to translocation into the nucleus and activate target genes [27]. While the deletion of Wnt/β-catenin reduced the proliferation of hair follicle progenitor cells and induced the early onset of the catagen phase [28], upregulation of Wnt/β-catenin signaling resulted in a more extensive hair growth [29], especially, Wnt-3α is sufficient to maintain DP cells in the anagen state [24]. In this work, the expression of Wnt-3α and β-catenin protein was increased in OSA and RBM group compared to control group.…”

Section: Resultsmentioning

confidence: 99%

Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Kim

Kwon

Jang

et al. 2017

Food & Nutrition Research

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The objective of this study is to evaluate of rice bran mineral extract (RBM) increases the expression of anagen-related molecules in human dermal papilla (DOCs). Four treatment groups were established to evaluate the efficacy of RBM, including a negative control, positive control (ascorbic acid), RBM and ortho-silicic acid (Si(OH)4) (OSA) group. Three days after the DPCs were administered the various treatments, western blot analysis showed that type I collagen expression was increased 2.5-fold in the OSA group and 4-fold in the RBM group, and ALP expression was increased 1.5-fold in the OSA and RBM group while the expression of fibronectin was increased ~3-fold in the OSA group and 2.5-fold in the RBM group. Also, the expression of Wnt-3α and β-catenin protein was increased in OSA and RBM group compared to control group. Furthermore, the expression of IL-1a was decreased by more than 50% in the OSA and RBM groups compared to the negative control. Analysis of mRNA expression by RT-qPCR showed that type I collagen increased 1.2-fold in the OSA- and RBM-treated DPCs, whereas type IV collagen increased 2.7-fold in the OSA group and 3.5-fold in the RBM group. However, TGF-β2 mRNA decreased about 80% in the OSA and RBM groups, respectively. Immunohistochemical staining of the DPCs for versican protein showed a significant increase in the OSA- and RBM-treated groups compared to the negative control. Thus, RBM have a potential to recover of DPCs activity and decreased inflammatory-related markers. It can be expected that hair loss prevention and hair growth enhancement can be expected when RBM is applied as a cosmetic product.

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Section: Resultsmentioning

confidence: 99%

Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Kim

Kwon

Jang

et al. 2017

Food & Nutrition Research

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“…In addition, various other compounds have demonstrated stimulatory effect on DPCs at molecular level .…”

Section: Hair Growth Promoting Effectmentioning

confidence: 99%

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Madaan

Verma

Singh

et al. 2018

Intern J of Cosmetic Sci

125

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Hair disorders such as hair loss (alopecia) and androgen dependent, excessive hair growth (hirsutism, hypertrichosis) may impact the social and psychological well-being of an individual. Recent advances in understanding the biology of hair have accelerated the research and development of novel therapeutic and cosmetic hair growth agents. Preclinical models aid in dermocosmetic efficacy testing and claim substantiation of hair growth modulators. The in vitro models to investigate hair growth utilize the hair follicle Dermal Papilla cells (DPCs), specialized mesenchymal cells located at the base of hair follicle that play essential roles in hair follicular morphogenesis and postnatal hair growth cycles. In this review, we have compiled and discussed the extensively reported literature citing DPCs as in vitro model to study hair growth promoting and inhibitory effects. A variety of agents such as herbal and natural extracts, growth factors and cytokines, platelet-rich plasma, placental extract, stem cells and conditioned medium, peptides, hormones, lipid-nanocarrier, light, electrical and electromagnetic field stimulation, androgens and their analogs, stress-serum and chemotherapeutic agents etc. have been examined for their hair growth modulating effects in DPCs. Effects on DPCs' activity were determined from untreated (basal) or stress induced levels. Cell proliferation, apoptosis and secretion of growth factors were included as primary end-point markers. Effects on a wide range of biomolecules and mechanistic pathways that play key role in the biology of hair growth were also investigated. This consolidated and comprehensive review summarizes the up-to-date information and understanding regarding DPCs based screening models for hair growth and may be helpful for researchers to select the appropriate assay system and biomarkers. This review highlights the pivotal role of DPCs in the forefront of hair research as screening platforms by providing insights into mechanistic action at cellular level, which may further direct the development of novel hair growth modulators.

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“…This clinical trial was designed to evaluate the efficacy of a formulated scalp shampoo among volunteers with androgenic alopecia. The formulated scalp shampoo was made by mixing IH extract and CS extract, both of which have been reported to possess hair growth-promoting constituents, such as costunolide and 3-deoxysappanchalcone, respectively [10,11,14]. As compared to the baseline hair count, the statistically significant increase in the hair density and total hair counts after 16 and 24 weeks of using scalp shampoo among the study subjects suggests that the IH and CS extract complex assisted in preventing hair loss and promoting hair growth.…”

Section: Discussionmentioning

confidence: 99%

“…We have recently reported that costunolide, a sesquiterpene lactone present in the roots of Inula helenium L. (Asteraceae), promotes hair growth in vivo, likely by activating intracellular signaling pathways including the Wnt/β-catenin, Shh/Gli, and TGF-β/Smad signaling pathways, which are associated with cell cycle regulation in hair follicular cells [10]. Moreover, 3-deoxysappanchalcone (3-DSC), a sesquiterpene lactone present in Caesalpinia Sappan L. (Leguminosae), has been shown to promote proliferation of dermal papillae and stimulate hair growth partly via activation of Wnt/β-catenin signaling and inhibition of signal transducer and activator of transcription-6 (STAT6)-mediated quiescence of hair follicular cells [11]. However, a clinical trial investigating the effect of extracts of Inula helenium (IH) and Caesalpinia Sappan (CS) on hair growth has not been performed yet.…”

Section: Introductionmentioning

confidence: 99%

Hair Growth Promotion by Extracts of Inula Helenium and Caesalpinia Sappan Bark in Patients with Androgenetic Alopecia: A Pre-clinical Study Using Phototrichogram Analysis

et al. 2019

Self Cite

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Inula helenium (IH) is known to possess antifungal, anti-bacterial, anti-helminthic, and anti-proliferation activities. Caesalpinia Sappan (CS) is known to reduce inflammation and improve blood circulation. Based on their folkloric use, these plants are expected to be promising candidates for promoting hair growth and preventing hair loss. Moreover, these plants are rich sources of certain phytochemicals, which have been reported to promote hair growth. In this clinical trial, we investigate the efficacy of a scalp shampoo formulated by mixing extracts of IH and CS in preventing hair loss and promoting hair growth in patients with androgenetic alopecia. Using a phototrichogram (Folliscope 2.8, LeadM, Korea), we compared the hair density and total hair counts in patients receiving the scalp shampoo at baseline, and at 8, 16, and 24 weeks after use of the shampoo. We found a statistically significant increase in the total hair count in the test group (n = 23) after 16 and 24 weeks of using the scalp shampoo (2.17 n/cm2 ± 5.72, p < 0.05; and 4.30 n/cm2 ± 6.37, p < 0.01, respectively) as compared to the control subjects. Based on the results of this clinical study, we conclude that the IH and CS extract complex is a promising remedy for preventing hair loss and promoting hair growth.

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3-Deoxysappanchalcone Promotes Proliferation of Human Hair Follicle Dermal Papilla Cells and Hair Growth in C57BL/6 Mice by Modulating WNT/β-Catenin and STAT Signaling

Cited by 32 publications

References 39 publications

Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Rice bran mineral extract increases the expression of anagen-related molecules in human dermal papilla through wnt/catenin pathway

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Hair Growth Promotion by Extracts of Inula Helenium and Caesalpinia Sappan Bark in Patients with Androgenetic Alopecia: A Pre-clinical Study Using Phototrichogram Analysis

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