3-deoxysilybin exerts anti-inflammatory effects by suppressing NF-κB activation in lipopolysaccharide-stimulated RAW264.7 macrophages

Cho, Byoung Ok; So, Yangkang; Jin, Chang Hyun; Nam, Bo Mi; Yee, Sung‐Tae; Jeong, Il Yun

doi:10.1080/09168451.2014.948377

Cited by 18 publications

(15 citation statements)

References 28 publications

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“…Caspase-8 works in two sides; the first one directly activates the downstream effector caspase-3, the second, caspase-8 induces the truncated Bid (tBid), which in turn results in the release of cytochrome C from the mitochondria into the cytosol. All these steps result in the activation of caspase-9 and caspase-3 [40]. The highest concentration of DEN (24 µg/mL) also resulted in decreased caspase 9, 8 and 3 levels.…”

Section: Discussionmentioning

confidence: 99%

Inducing G2/M Cell Cycle Arrest and Apoptosis through Generation Reactive Oxygen Species (ROS)-Mediated Mitochondria Pathway in HT-29 Cells by Dentatin (DEN) and Dentatin Incorporated in Hydroxypropyl-β-Cyclodextrin (DEN-HPβCD)

Ashwaq

Al‐Qubaisi

Abdullah

et al. 2016

IJMS

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Dentatin (DEN), purified from the roots of Clausena excavata Burm f., has poor aqueous solubility that reduces its therapeutic application. The aim of this study was to assess the effects of DEN-HPβCD (hydroxypropyl-β-cyclodextrin) complex as an anticancer agent in HT29 cancer cell line and compare with a crystal DEN in dimethyl sulfoxide (DMSO). The exposure of the cancer cells to DEN or DEN-HPβCD complex leads to cell growth inhibition as determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. To analyze the mechanism, in which DEN or DEN-HPβCD complex causes the death in human colon HT29 cancer cells, was evaluated by the enzyme-linked immunosorbent assay (ELIZA)-based assays for caspase-3, 8, 9, and reactive oxygen species (ROS). The findings showed that an anti-proliferative effect of DEN or DEN-HPβCD complex were via cell cycle arrest at the G2/M phase and eventually induced apoptosis through both mitochondrial and extrinsic pathways. The down-regulation of poly(ADP-ribose) polymerase (PARP) which leaded to apoptosis upon treatment, was investigated by Western-blotting. Hence, complexation between DEN and HPβCD did not diminish or eliminate the effective properties of DEN as anticancer agent. Therefore, it would be possible to resolve the conventional and current issues associated with the development and commercialization of antineoplastic agents in the future.

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Section: Discussionmentioning

confidence: 99%

Inducing G2/M Cell Cycle Arrest and Apoptosis through Generation Reactive Oxygen Species (ROS)-Mediated Mitochondria Pathway in HT-29 Cells by Dentatin (DEN) and Dentatin Incorporated in Hydroxypropyl-β-Cyclodextrin (DEN-HPβCD)

Ashwaq

Al‐Qubaisi

Abdullah

et al. 2016

IJMS

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“…These free radicals are reduced by flavonoids (Tattini et al, ; Treutter, ). In addition, flavonoids also have anti‐tumor (Falcone Ferreyra et al, ; Middleton, Kandaswami, & Theoharides, ; Russo, Russo, & Spagnuolo, ), anti‐inflammatory (Cheng, Zhang, Gao, & Zhang, ; Cho et al, ), anti‐allergic (Kritas et al, ), anti‐thrombotic (Lee, Ku, & Bae, ), anti‐diabetic (Gaur, Yadav, Verma, Yadav, & Bhakuni, ) and anti‐atherosclerotic activities (Salvamani, Gunasekaran, Shaharuddin, Ahmad, & Shukor, ).…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of chickpea roots reveal differential expression of abscisic acid responsive proteins

2019

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Seeds of chickpea are nutritious and alleged to be a preferred source of protein next only to milk. Some of the biotic and abiotic factors reduce chickpea production worldwide. Plant roots are the first to perceive stress signals. The wild root free radical scavenging activity measured by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) method was 28.06 ± 1.43% and 25.12 ± 0.95% in cultivated chickpea type. The root proteins were resolved on 7 cm IPG strip having a pH gradient 5–8 and subsequently separated on the basis of mass using SDS–PAGE in second dimension. A total of eight representative spots were subjected for identification by MALDI‐TOF‐MS. A protein‐protein association network analysis using STRING software permitted to build an interactomic map of all detected proteins, characterised by 16 interactions. The findings may provide a better understanding of the biochemical mechanism of different root pathways and stress‐responses in chickpea. Practical applications Information pertaining to stress resistance is essential from breeder’s perspectives. Chickpea is prone to high yield losses due to recurring droughts. MALDI‐TOF‐MS coupled with MASCOT query search found significant correlations with abscisic acid responsive proteins associated to drought stress using comparative proteomics. This report will assist researchers a ready reference for executing further studies concerning chickpea root proteins. The findings may provide a better understanding of the biochemical mechanism of different root pathways and stress‐responses in chickpea.

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“…In addition, TNF-α-enhanced COX-2 and NF-κB p65 levels were suppressed by culture with these three botanical molecules in combination (15). LPS treatment has been reported to enhance the production of TNF-α, IL-6 and IL-1β due to stimulating signaling pathways linked to MAPK and NF-κB p65 in macrophage RAW264.7 cells (31)(32)(33). It is possible that the combination of (+)-catechin, baicalin and β-caryophyllene may exhibit potent suppressive effects on the LPS-enhanced production of inflammatory cytokines by inhibiting signaling pathways involved in MAPK and NF-κB p65 in the activated macrophages.…”

Section: Discussionmentioning

confidence: 99%

The combination of catechin, baicalin and β‑caryophyllene potentially suppresses the production of inflammatory cytokines in mouse macrophages in vitro

Yamaguchi

Levy

2019

Exp Ther Med

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It has been demonstrated that the combination of three botanical factors of (+)-catechin, baicalin and β-caryophyllene, which exhibit anti-inflammatory effects, with comparatively lower concentrations of each factor, demonstrating a potent synergistic-suppressive effect on the growth of mouse macrophage RAW264.7 cells in vitro, and suggesting it may function as a pharmacologic tool for managing inflammatory diseases. The present study was undertaken to determine the suppressive effects of (+)-catechin, baicalin or β-caryophyllene on the production of inflammatory cytokines, including TNF-α, IL-6 and IL-1β, which was enhanced by lipopolysaccharide (LPS) in RAW264.7 cells in vitro. The cells were cultured for 3 days without botanical factors, followed by incubation for 5 h in the presence of either vehicle, (+)-catechin [1 µg/ml (3.45 µM)], baicalin [1 µg/ml (2.24 µM)], or β-caryophyllene [1 µg/ml (5 µM)] with or without LPS (100 ng/ml); this did not have significant effects on the number of RAW264.7 cells. The production of TNF-α, IL-6 and IL-1β was not altered by the addition of (+)-catechin, baicalin, β-caryophyllene, or the three combined factors in RAW264.7 cells without LPS. LPS treatment caused a marked production of TNF-α, IL-6, and IL-1β. This enhancement was suppressed by the addition of (+)-catechin, baicalin or β-caryophyllene. Of note, the production of these cytokines was additively suppressed by the combination of the three factors in macrophages. Thus, the combination of (+)-catechin, baicalin and β-caryophyllene was found to reveal a potent suppressive effect on cytokine production in macrophages in vitro. This composition may be a useful tool as a potent anti-inflammatory agent.

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3-deoxysilybin exerts anti-inflammatory effects by suppressing NF-κB activation in lipopolysaccharide-stimulated RAW264.7 macrophages

Cited by 18 publications

References 28 publications

Inducing G2/M Cell Cycle Arrest and Apoptosis through Generation Reactive Oxygen Species (ROS)-Mediated Mitochondria Pathway in HT-29 Cells by Dentatin (DEN) and Dentatin Incorporated in Hydroxypropyl-β-Cyclodextrin (DEN-HPβCD)

Inducing G2/M Cell Cycle Arrest and Apoptosis through Generation Reactive Oxygen Species (ROS)-Mediated Mitochondria Pathway in HT-29 Cells by Dentatin (DEN) and Dentatin Incorporated in Hydroxypropyl-β-Cyclodextrin (DEN-HPβCD)

Proteomic analysis of chickpea roots reveal differential expression of abscisic acid responsive proteins

The combination of catechin, baicalin and β‑caryophyllene potentially suppresses the production of inflammatory cytokines in mouse macrophages in vitro

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