The combination of catechin, baicalin and β‑caryophyllene potentially suppresses the production of inflammatory cytokines in mouse macrophages in vitro

Yamaguchi, Masayoshi; Levy, Robert M.

doi:10.3892/etm.2019.7452

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…The anti-inflammatory activity of açaí is often associated with the presence of flavonoids, such as catechin and epicatechin, both in the pulp fruit [ 18 ] and in the seeds [ 19 ]. Catechin’s anti-inflammatory effect is well described alone, acting via the regulation of Toll-like receptor (TLR) 2/4 and inflammasome signaling [ 20 ], or in combination with other compounds, suppressing the production of inflammatory cytokines in mouse macrophages in vitro in association with baicalin and β-caryophyllene [ 21 ], and inhibiting the activation of TLR4-MyD88-mediated nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen activated protein kinase (MAPK) signaling pathways in RAW 264.7 macrophages in association with quercetin [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Xavier

Teles

Tellis³

et al. 2021

Foods

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Açaí berry is a fruit from the tree commonly known as açaízeiro (Euterpe oleracea Mart.) originated from the Amazonian region and widely consumed in Brazil. There are several reports of the anti-inflammatory activity of its pulp and few data about the seed’s potential in inflammation control. This work aimed to evaluate the effect of catechin-rich açaí extract on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and carrageenan-induced paw edema. The treatment with E. oleracea ethyl acetate extract (EO-ACET) was used in an in vitro model performed with macrophages stimulated by LPS, in which pro-inflammatory markers were evaluated, and in an in vivo model of acute inflammation, in which edema inhibition was evaluated. EO-ACET showed an absence of endotoxins, and did not display cytotoxic effects in RAW 264.7 cells. LPS-stimulated cells treated with EO-ACET displayed low levels of nitrite and interleukins (IL’s), IL-1β, IL-6 and IL-12, when compared to untreated cells. EO-ACET treatment was able to inhibit carrageenan-induced paw edema at 500 and 1000 mg/kg, in which no acute inflammatory reaction or low mast cell counts were observed by histology at the site of inoculation of λ-carrageenan. These findings provide more evidence to support further studies with E. oleracea seeds for the treatment of inflammation.

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Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Xavier

Teles

Tellis³

et al. 2021

Foods

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“…In the same study, β-caryophyllene improved the reduction of the macrophage number induced by metaxalone [30]. Similarly, Yamaguchi et al [31] have found that a short 5 h exposure to a low concentration (5 µM) of β-caryophyllene counteracted the LPS-induced release of pro-inflammatory cytokines without affecting the basal levels of IL-1 β, IL-6, and TNF-α, but lowered them after LPS stimulation. Similarly, a 48 h exposure to α-humulene, at concentrations of 755 and 1555 µM, inhibited the inflammatory response induced by LPS, by lowering the release of nitric oxide (NO), in murine macrophages RAW 264.7 [32].…”

Section: Ability Of Caryophyllane Sesquiterpenes To Modulate Macropha...mentioning

confidence: 70%

Novel Insights into the Immunomodulatory Effects of Caryophyllane Sesquiterpenes: A Systematic Review of Preclinical Studies

et al. 2022

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Immunomodulation is a key factor in the homeostasis of organisms, both for physiological and inflammatory conditions. In this context, great attention has been devoted to immunomodulant agents, which can boost or modulate the immune system, thus favoring disease relief. The present systematic review is focused on the immunomodulatory properties of plant-based caryophyllane sesquiterpenes, which are unique natural compounds widely studied due to their multiple and pleiotropic bioactivities. Despite lacking clinical evidence, the selected studies highlighted the ability of these substances, especially β-caryophyllene and α-humulene, to modulate the immune system of both in vitro and in vivo models of disease, such as neurodegenerative and inflammatory-based diseases, cancer, and allergies; moreover, some mechanistic hypotheses have been made too. The present overview suggests a further interest in immunomodulation by caryophyllane sesquiterpenes as a possible novel strategy for immune-based diseases or as an adjuvant treatment and encourages further high-quality studies, using high-purity compounds, to better clarify the mechanisms accounting for these properties and to support a further pharmaceutical development.

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“…Curcumin and Resveratrol (RSV) have been deeply described for their anti-inflammatory property both in monocytic and endothelial cells (Rana et al 2016 ; Schwager et al 2017 ; Sun et al 2015 ), whereas BCP was only recently identified as an anti-inflammatory compound (Yamaguchi and Levy 2019 ). However, to the best of our knowledge, this is the first study evaluating the effect in vitro of bCUR, RSV and, BCP combined together (data are summarized in Table 1 ), as described in the novel dietary supplement Fenoxidol™.…”

Section: Discussionmentioning

confidence: 99%

Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells

et al. 2021

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A challenging and promising new branch of aging-related research fields is the identification of natural compounds able to modulate the senescence-associated secretory phenotype (SASP), which characterizes senescent cells and can contribute to fuel the inflammaging. We investigated both the anti-SASP and anti-inflammatory activities of a nutritional supplement, namely Fenoxidol™, composed of turmeric extract bioCurcumin (bCUR), Polydatin (the natural glycosylated precursor of Resveratrol-RSV), and liposomal β-caryophyllene (BCP), in two human cellular models, such as the primary endothelial cell line, HUVECs and the monocytic cell line, THP-1. Replicative and Doxorubicin-induced senescent HUVECs, both chosen as cellular models of SASP, and lipopolysaccharides (LPS)-stimulated THP-1, selected as a model of the inflammatory response, were treated with the three single natural compounds or with a combination of them (MIX). In both senescent HUVEC models, MIX treatment significantly reduced IL-1β and IL-6 expression levels and p16ink4a protein, and also increased SIRT1 protein level, as well as downregulated miR-146a and miR-21 expression, two of the so-called inflamma-miRNAs, more effectively than the single compounds. In THP-1 cells stimulated with LPS, the MIX showed a significant effect in decreasing IL-1β, IL-6, TNF-α, and miR-146a expression levels and Caspase-1 activation, in association with an up-regulation of SIRT1 protein, compared to the single compounds. Overall, our results suggest that the three analysed compounds can have a combined effect in restraining SASP in senescent HUVECs as well as the inflammatory response in LPS-stimulated THP-1 cells.

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The combination of catechin, baicalin and β‑caryophyllene potentially suppresses the production of inflammatory cytokines in mouse macrophages in vitro

Cited by 8 publications

References 34 publications

Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Novel Insights into the Immunomodulatory Effects of Caryophyllane Sesquiterpenes: A Systematic Review of Preclinical Studies

Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells

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