3′ end of eae gene-based fluorescence DNA nanosensor for detection of E. coli O157:H7

Karimi, Farrokh; Balazadeh, Nasrin; Eftekhari‐Sis, Bagher

doi:10.1007/s13353-019-00511-0

Cited by 4 publications

(1 citation statement)

References 37 publications

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“…To date, considerable effort has been invested in developing effective and appropriate strategies for the detection of E. coli O157:H7. However, established methods are not suitable for on-site inspection because of false positives and the need for specialized equipment (Li 2016;Karimi et al 2019). Quantitative PCR has high sensitivity and good reliability, but the fluorescent-labeled probe needed to ensure specificity and amplicon detection requires expensive instrumentation to detect (Bosilevac et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Escherichia coli O157:H7 by Loop-Mediated Isothermal Amplification Coupled with a Lateral Flow Assay Targeting the z3276 Genetic Marker

et al. 2021

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The most commonly reported serotype of Shiga toxin-producing Escherichia coli (STEC) is O157:H7. This pathogen presents a threat to public health and is a cause of foodborne illness worldwide. The efficient and sensitive detection of E. coli O157:H7 remains a challenge for food safety. In this report, we developed a sensitive and specific loop-mediated isothermal amplification (LAMP) reaction coupled with a lateral flow (LF) assay to rapidly detect E. coli O157:H7. Six specific primers were targeted to the genetic marker z3276. Two loop primers were labeled with either fluorescein isothiocyanate (FITC) or digoxin (Dig), which facilitated analysis by the LF assay. The LAMP-LF assay detected E. coli O157:H7, and no crossreactivity was observed with the other bacterial strains tested, including non-O157 STEC and other E. coli. The detection limit of the LAMP-LF assay was 1.3 CFU/mL for E. coli O157:H7 in broth culture, which was of equal or higher sensitivity than the other amplicon detection methods tested (agarose electrophoresis and dye). Analysis of samples from slaughterhouses indicted that the z3276-LAMP-LF assay was superior in terms of sensitivity, accuracy, and rapidity in detecting E. coli O157:H7 compared with conventional PCR. A further advantage of this method is that it is not dependent upon specialized equipment or techniques and therefore has wide potential applications in the field.

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Section: Introductionmentioning

confidence: 99%