3-Hydroxypropionaldehyde, an inhibitory metabolite of glycerol fermentation to 1,3-propanediol by enterobacterial species

Barbirato, Fabien; Grivet, Jean‐Philippe; Soucaille, Philippe; Bories, A.

doi:10.1128/aem.62.4.1448-1451.1996

Cited by 148 publications

(61 citation statements)

References 9 publications

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“…The accumulation of 1,3-propanediol, a typical product of glycerol fermentation not found in anaerobic conversions of other organic substrates (Biebl et al, 1999), showed that glycerol was metabolized via the reuterin pathway. Although it is believed that some strains of L. reuteri are unique among propanediol producers in their ability to accumulate reuterin from glycerol (Casas & Dobrogosz, 2000), few enterobacteria and C. butyricum can accumulate reuterin during in vitro fermentation of glycerol (Barbirato et al, 1996;Gonzalez-Pajuelo et al, 2005). These data suggest that small amounts of reuterin were produced in R1 during addition of glycerol by intestinal propanediol-producers such as C. butyricum or commensal L. reuteri, because small concentrations of L. reuteri were detected in fermentation samples from R1.…”

Section: Discussionmentioning

confidence: 99%

Glycerol induces reuterin production and decreases Escherichia coli population in an in vitro model of colonic fermentation with immobilized human feces

Cleusix¹,

Lacroix²,

Vollenweider³

et al. 2008

FEMS Microbiology Ecology

116

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Lactobacillus reuteri ATCC 55730 is a probiotic strain that produces, in the presence of glycerol, reuterin, a broad-spectrum antimicrobial substance. This strain has been shown to prevent intestinal infections in vivo; however, its mechanisms of action, and more specifically whether reuterin production occurs within the intestinal tract, are not known. In this study, the effects of L. reuteri ATCC 55730 on intestinal microbiota and its capacity to secrete reuterin from glycerol in a novel in vitro colonic fermentation model were tested. Two reactors were inoculated with adult immobilized fecal microbiota and the effects of daily addition of L. reuteri into one of the reactors (c.10(8) CFU mL(-1)) without or with glycerol were tested on major bacterial populations and compared with addition of glycerol or reuterin alone. The addition of glycerol alone or with L. reuteri increased numbers of the Lactobacillus-Enterococcus group and decreased Escherichia coli. The addition of reuterin significantly and selectively decreased E. coli without affecting other bacterial populations. The observed decrease in E. coli concentration during the addition of glycerol (in presence or absence of L. reuteri) could be due to in situ reuterin production because 1,3-propanediol, a typical product of glycerol fermentation, was detected during the addition of glycerol.

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Section: Discussionmentioning

confidence: 99%

Glycerol induces reuterin production and decreases Escherichia coli population in an in vitro model of colonic fermentation with immobilized human feces

Cleusix¹,

Lacroix²,

Vollenweider³

et al. 2008

FEMS Microbiology Ecology

116

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“…This is in accordance with the findings of Boenigk et al (1993) and Abbad-Andaloussi et al (1996) for 1,3-PD formation in C. freundii and C. butyricum, respectively. It has been argued that the physiological meaning of this limiting step is to avoid the accumulation of 3hydroxypropionaldehyde (3-HPA), which was found to be very toxic to the growth of another 1,3-PD-producing microorganism Enterobacter agglomerans (Barbirato et al, 1996). More experimental investigations are needed to address the possible accumulation of 3-HPA in other 1,3-PDproducing strains.…”

Section: In Vitro and In Vivo Enzyme Activities Under Steady Statesmentioning

confidence: 99%

Kinetic, dynamic, and pathway studies of glycerol metabolism byKlebsiella pneumoniae in anaerobic continuous culture: III. Enzymes and fluxes of glycerol dissimilation and 1,3-propanediol formation

Ahrens

Menzel

Zeng

et al. 1998

Biotechnol. Bioeng.

130

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The initial steps of glycerol dissimilation and 1,3‐propanediol (1,3‐PD) formation by Klebsiella pneumoniae anaerobically grown on glycerol were studied by quantifying the in vitro and in vivo activities of enzymes in continuous culture under conditions of steady state and oscillation and during transient phases. The enzymes studied included glycerol dehydrogenase (GDH), glycerol dehydratase (GDHt), and 1,3‐propanediol oxidoreductase (PDOR). Three conclusions can be drawn from the steady‐state results. First, glycerol concentration in the culture is a key parameter that inversely affects the in vitro activities (concentrations) of all three enzymes, but has a positive effect on their in vivo activities. Growth rate significantly affects the ratio of in vitro and in vivo enzyme activities under low glycerol concentrations, but not under glycerol excess. Second, whereas the flux through the oxidative pathway of glycerol dissimilation is governed mainly by the regulation of in vivo enzyme activity on a metabolic level, the flux through the reductive pathway is largely controlled by the synthesis of enzymes. Third, GDHt is a major rate‐liming enzyme for the consumption of glycerol and the formation of 1,3‐PD in K. pneumoniae at high glycerol concentrations. Results from oscillating cultures revealed that both in vitro and in vivo activities of the enzymes oscillated. The average values of the in vitro activities during an oscillation cycle agreed well with their corresponding values for nonoscillating cultures under similar environmental conditions. Experiments with step changes in the feed concentration of glycerol demonstrated that growth and product formation are very sensitive to changes of substrate concentration in the culture. This sensitivity is due to the dynamic responses of the genetic and metabolic networks. They should be considered when modeling the dynamics of the culture and attempting to improve the formation of 1,3‐PD. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59: 544–552, 1998.

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“…Specific environmental conditions that led to dominance of one fermentation pathway over another (i.e., propionic and isovaleric acid accumulation vs. lactic and formic acid accumulation) appear to have been a determining factor affecting perchlorate biodegradation. Accumulation of inhibitory glycerin fermentation products, such as 3-hydroxylpropionaldehyde, may have also occurred (Barbirato et al, 1996).…”

Section: Resultsmentioning

confidence: 99%

Rapid full‐scale bioremediation of perchlorate in soil at a large brownfields site

Evans

Moore

et al. 2008

Remediation Journal

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The former Bermite site north of Los Angeles, California, was used to manufacture various explosives and related products containing energetic compounds, including perchlorate. Remediation of perchlorate in site soil and groundwater is being conducted to meet regulatory requirements and allow planned redevelopment activities to proceed. The general approach to perchlorate remediation of shallow soil at the site includes excavation of affected soils followed by ex situ bioremediation. Glycerin was chosen for use as an electron donor because of its stability, safety, low cost, and regulatory acceptance. However, full-scale bioremediation operation with glycerin initially resulted in inconsistent results despite consistent perchlorate biodegradation observed in treatability study microcosms. To eliminate the inconsistency and optimize the biotreatment process, additional studies were performed in the field on parallel tracks to determine crucial factor(s) that influenced inconsistent breakdown of perchlorate in site soils. Total Kjeldahl nitrogen (TKN) was determined to be a significant factor limiting perchlorate biodegradation. The addition of di-ammonium phosphate (DAP) resulted in the consistent and complete perchlorate removal, generally within two weeks of incubation with a median destruction rate of about 200 µg/kg/day.Soil processing rates were gradually increased over the year, and, by the summer, approximately 2,000 to 2,500 tons of soil were being processed per day with a total of approximately 160,000 tons processed by the end of July. The total unit treatment cost for the process is about approximately $35/ton. The glycerin-DAP process is playing a major role in the remediation of this 1,000-acre former industrial site. O

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3-Hydroxypropionaldehyde, an inhibitory metabolite of glycerol fermentation to 1,3-propanediol by enterobacterial species

Cited by 148 publications

References 9 publications

Glycerol induces reuterin production and decreases Escherichia coli population in an in vitro model of colonic fermentation with immobilized human feces

Glycerol induces reuterin production and decreases Escherichia coli population in an in vitro model of colonic fermentation with immobilized human feces

Kinetic, dynamic, and pathway studies of glycerol metabolism byKlebsiella pneumoniae in anaerobic continuous culture: III. Enzymes and fluxes of glycerol dissimilation and 1,3-propanediol formation

Rapid full‐scale bioremediation of perchlorate in soil at a large brownfields site

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