3-Mercaptopropionic acid capped ZnSe quantum dot-cytochrome P450 3A4 enzyme biotransducer for 17β-estradiol

Ndangili, Peter M.; Jijana, Abongile Nwabisa; Baker, Priscilla; Iwuoha, Emmanuel I.

doi:10.1016/j.jelechem.2010.12.029

Cited by 29 publications

(19 citation statements)

References 51 publications

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“…In an electrochemical system, the resistance of electrolyte solution is related to the bulk properties of the electrolyte solution. Besides, Warburg impedance represents the diffusion of the redox probe through the electrode surface (Ndangili et al, 2011;Fang et al, 2014;Bahadır and Sezgintürk, 2014;Özcan et al, 2014). Table 3 summarizes impedimetric biosensors for hormone detection in literature.…”

Section: Impedimetric Biosensors For Hormonesmentioning

confidence: 99%

“…When compared to these technique electrochemical techniques, known as conventional analytical methods, have many advantages in terms of low cost, high sensitivity, fast response, and easy operation for hormone detection. Also these techniques do not require expensive instrumentation and specialized personnel (Ndangili et al, 2011;Volpe et al, 2006;Ke et al, 2014;Lu et al, 2007). Biosensors are economical and fast tools for clinical and pharmaceutical studies.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical biosensors for hormone analyses

Bahadır

Sezgintürk

2015

Biosensors and Bioelectronics

148

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Section: Impedimetric Biosensors For Hormonesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrochemical biosensors for hormone analyses

Bahadır

Sezgintürk

2015

Biosensors and Bioelectronics

148

View full text Add to dashboard Cite

“…, and the second electron is for splitting the O-O bond of the PZA-CYP2E1-O 2 intermediate [26,27]. The sensitivity of the biosensor is very much enhanced by the incorporation of MWCNTs into the MWCNT-PANI nanocomposite platform used in the construction of the nanobiosensor.…”

Section: +mentioning

confidence: 99%

Polyanilino-Carbon Nanotubes Derivatised Cytochrome P450 2E1 Nanobiosensor for the Determination of Pyrazinamide Anti-Tuberculosis Drugs

Sidwaba

Ajayi

Feleni

et al. 2014

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Abstract. Pyrazinamine (PZA) is one of the most commonly prescribed anti-tuberculosis (anti-TB) drug due to its ability to significantly shorten the TB treatment period. However, excess PZA in the body causes hepatotoxicity and liver damage. This, therefore, calls for new methods for ensuring reliable dosing of the drug, which will differ from person to person due to interindividual differences in drug metabolism. A novel biosensor system for monitoring the metabolism of PZA was prepared with nanocomposite of multi-walled carbon nanotubes (MWCNTs), polyaniline (PANI) and cytochrome P450 3A4 (CYP3A4) electrochemically deposited on a glassy carbon electrode (GCE).The nanocomposite biosensor system exhibited enhanced electroactivity that is attributable to the catalytic effect of the incorporated MWCNTs. The biosensor had a sensitivity of 7.80 µA/µg mL -1 PZA and a dynamic linear range of 4.92 -160 ng/mL PZA.

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“…Several biosensors have been developed for the quantification of endocrine disruptors such as 17β-estradiol [11,12,13] using different electrode modification techniques.…”

Section: Introductionmentioning

confidence: 99%

“…The small sizes of the nanoparticles increase the surface area of the electrode thus affording more binding sites for the enzyme. A high amount of enzyme on the electrode surface increases the rate of diffusion of the analyte towards the electrode [11]. Also, the carboxylic group offers a biocompatible surface since it can react favourably with the amino group of enzyme without loss of enzyme activity.…”

mentioning

confidence: 99%

3-Mercaptopropionic Acid Capped Ga<sub>2</sub>Se<sub>3</sub>nanocrystal-CYP3A4 Biosensor for the Determination of 17-Alpha-Ethinyl Estradiol in Water

Ndangili

Nxusani

Olowu

et al. 2012

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Abstract. Water soluble and biocompatible 3-mercaptopropionic acid capped gallium selenide nanocrystals, were synthesized from hydrated gallium (III) perchlorate and selenide ions. The 3-mercaptopropionic acid capped gallium selenide nanocrystals, was non-fluorescent but showed a sharp UV-vis absorption maximum at 250 nm.The synthesized nanoparticle was used to develop an electrochemical biosensor for the detection of 17-alpha-ethinyl estradiol, an estrogenic endocrine disrupting compound (e-EDC). The biosensor was fabricated by potentiostatic deposition of novel gallium selenide nanocrystals on a L-cystine modified gold electrode, followed by covalent coupling of genetically engineered cytochrome P450-3A4 (CYP3A4), a Heme containing enzyme.The biosensor gave an electrochemical response at about -220 mV. The results revealed that 3-Mecarptopropanoic acid capped Gallium Selenide nanocrystals can be used in conjunction with CYP3A4 as an electrode modifier for the detection of 17-alpha ethinyl estradiol. The 3-Mecarptopropanoic acid capped Gallium Selenide nanoparticles exhibited a semiconductor like behaviour.

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3-Mercaptopropionic acid capped ZnSe quantum dot-cytochrome P450 3A4 enzyme biotransducer for 17β-estradiol

Cited by 29 publications

References 51 publications

Electrochemical biosensors for hormone analyses

Electrochemical biosensors for hormone analyses

Polyanilino-Carbon Nanotubes Derivatised Cytochrome P450 2E1 Nanobiosensor for the Determination of Pyrazinamide Anti-Tuberculosis Drugs

3-Mercaptopropionic Acid Capped Ga<sub>2</sub>Se<sub>3</sub>nanocrystal-CYP3A4 Biosensor for the Determination of 17-Alpha-Ethinyl Estradiol in Water

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