Polyanilino-Carbon Nanotubes Derivatised Cytochrome P450 2E1 Nanobiosensor for the Determination of Pyrazinamide Anti-Tuberculosis Drugs

Abstract

Abstract. Pyrazinamine (PZA) is one of the most commonly prescribed anti-tuberculosis (anti-TB) drug due to its ability to significantly shorten the TB treatment period. However, excess PZA in the body causes hepatotoxicity and liver damage. This, therefore, calls for new methods for ensuring reliable dosing of the drug, which will differ from person to person due to interindividual differences in drug metabolism. A novel biosensor system for monitoring the metabolism of PZA was prepared with nanocomposite of multi-walled carbon nanotubes (MWCNTs), polyaniline (PANI) and cytochrome P450 3A4 (CYP3A4) electrochemically deposited on a glassy carbon electrode (GCE).The nanocomposite biosensor system exhibited enhanced electroactivity that is attributable to the catalytic effect of the incorporated MWCNTs. The biosensor had a sensitivity of 7.80 µA/µg mL -1 PZA and a dynamic linear range of 4.92 -160 ng/mL PZA.

An Amperometric Cytochrome P450-2D6 Biosensor System for the Detection of the Selective Serotonin Reuptake Inhibitors (SSRIs) Paroxetine and Fluvoxamine

“…Where L represents the polymer thickness, n represents the number of electrons involved in the reaction, F is the Faraday constant (96,584 C/mol), Г*PANSA is the surface concentration of the PANSA film (mol cm -2 ), ν the scan rate (mV/s), A the electrode area (cm 2 ), I p the peak current (A), R is the universal gas constant (8.314 J mol K-1), and T is the temperature of the system (K). Compared to the D e values reported by [42], this study gives evidence of a more conductive polymeric film which is as a consequence of the sulphonic substituent in PANSA, thus illustrating a faster electron transfer. From (inset of Fig.…”