3-nitrotyrosine levels in dichlorvos-induced neurotoxicity

Abstract

The aim of this study was to evaluate dichlorvos toxicity in terms of nitro-oxidative stress by determining 3-nitrotyrosine (3-NT) levels in the fore, mid, and hindbrain regions in acutely exposed rats. Male SpragueDawley rats were randomly allocated to three groups of eight. Group 1 was administered a single intraperitoneal dichlorvos dose of 1.8 mg kg -1 (0.1xLD 50 ) and group 2 a dose of 9 mg kg -1 (0.5xLD 50 ). The control group received 0.5 mL saline solution via the same route. 3-NT and tyrosine (TYR) levels were measured using high performance liquid chromatography with a photodiode array detector (HPLC-PDA) and expressed as a ratio of 3-NT to TYR. The 3-NT/1000 TYR ratios increased significantly in the fore-, mid-and hindbrains of the exposed groups compared to control (p<0.01). In the forebrain, the increase was also significant between the treated groups. Our study has confirmed that acute exposure to dichlorvos leads to nitro-oxidative stress in the brain and that 3-NT may play a role in the mechanism of dichlorvos neurotoxicity.

The effect of copper nanoparticles and copper (II) salt on redox reactions and epigenetic changes in a rat model

“…3‐Nitrotyrosine (3‐NT), on the other hand, is the result of post‐translational protein modification by reactive nitrogen species (RNS) such as nitric oxide (NO), derived oxidants (e.g., peroxynitrite (ONOO − ) and peroxynitrous acid (ONOOH)) and nitrogen dioxide radicals (•NO 2 ) (Teixeira, Fernandes, Prudêncio, & Vieira, ). An increase in PC and 3‐NT content in the body is observed during oxidative stress in cells (Bailey, Patel, Young, & Asayama, ; Guvenç, Aksoy, Das, Atmaca, & Yavuz, ; Liu & He, ; Patel & Kalia, ). Our research has shown that intake of Cu in the form of CuCO 3 or CuNP in the rat diet, both in the recommended amount and reduced by half, decreased plasma PC but only application of Cu from CuNP in the diet decreased plasma 3‐NT content as compared to rats whose diet was not supplemented with Cu.…”

Polyphenol-rich fraction of Parquetina nigrescens mitigates dichlorvos-induced neurotoxicity and apoptosis

“…This is in agreement with Guvenc et al. [24] who reported the induction of oxidative stress with dichlorvos toxicity. In the cerebellum of rats treated with 100 mg/kg prf of P. nigrescens , a significant increase in SOD compared to the toxicant group was observed with concomitant increases in CAT and GPx activity.…”

3-Nitrotyrosine quantification methods: Current concepts and future challenges

“…Since 3-NT was suggested as a biomarker of nitrosative stress, a substantial effort has been made to develop analytical methods that can be applied to biological samples [64]. Accurate quantification of substances present in biological samples at very low concentrations is, indeed, a complex task, and the particular case of 3-NT requires special concerns [1].…”