3-Oxoteasterone and the Epimerization of Teasterone: Identification in Lily Anthers andDistylium racemosumLeaves and Its Biotransformation into Typhasterol

BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis

“…For example, 3,24‐diepiCS and 3,24‐diepiBL were found to be conjugated to 3‐laurate, 3‐myristate or 3‐palmitate ester in serradella cells ( Ornithopus sativus ) (Kolbe et al ., , ). TE and its derivatives may be conjugated to 3‐laurate and 3‐myristate, creating several acyl metabolites in lily ( Lilium longiflorum ) cell cultures (Abe et al ., ; Asakawa et al ., , ; Kolbe et al ., ; Soeno et al ., ,b). These acyl BRs are easily converted to free TE, CS and BL intermediates by hydrolysis.…”

Arabidopsis CYP85A2 Catalyzes Lactonization Reactions in the Biosynthesis of 2-Deoxy-7-oxalactone Brassinosteroids

“…Â 250 mm; Nomura Chemical, Aichi, Japan) with a CH 3 CN-H 2 O solvent system. 16) The fractions that eluted from 16-18 min and 22-24 min contained 7-OXTE and 7-OXTY, respectively, and were further purified by using normalphase HPLC (Senshu Pak Aquasil SS-352N, 4.6 mm i.d. Â 250 mm, Senshu Scientific, Tokyo) at a flow rate of 1 ml min À1 with n-hexane:2-propanol at 85:15 containing 0.5% H 2 O. Eluates containing 2-deoxy BRs were converted to a methyl boronate ester and then to a TMSi ether with TMCS.…”

Medicinal and Aromatic Plants IX

“…Recently, 3-dehydroteasterone was identified as one of brassinosteroids in wheat (Triticum aestivum L.) grains (Yokota et al 1994) and lily (Lilium longiflorum cv. Bioconversion of 3-dehydroteasterone to typhasterol was shown using cell cultures of lily, which did not produce any brassinosteroids (Abe et al 1994). Bioconversion of 3-dehydroteasterone to typhasterol was shown using cell cultures of lily, which did not produce any brassinosteroids (Abe et al 1994).…”