3'-Terminal nucleotide sequence of alfalfa mosaic virus RNA 4.

Koper-Zwarthoff, Ellen C.; Bol, John F.

doi:10.1073/pnas.76.3.1114

Cited by 16 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The finding that the homologous region contains several hairpins with a rather negative AG (see also ref. 6) may explain the fact that no homology terminal 88 nucleotides of AlMV RNA 4 (5,6). The present results imply that in ALMV RNAs 1, 2 and 3 specific binding of coat protein also occurs close to the 3'-termini of these RNA species.…”

Section: Discussionmentioning

confidence: 56%

“…Digestion with RNase Ti from Sankyo under the same conditions yielded a more rigourous degradation and was used to produce RNA 4 fragments with a length of 25 to 100 nucleotides. The digests were subjected to zonal gradient centrifugation (6) and fractions enriched in fragment 69 or in fragments with a length of 25 to 100 nucleotides were collected. The fragments were concentrated by ethanol precipitation and labeled at the 5'-end with polynucleotide kinase as described (7).…”

Section: Van Boom (Leiden)mentioning

confidence: 99%

“…The sequence of 5'-labeled RNA 4 fragments was deduced by a combination of three methods: (a) twodimensional electrophoresis/homochromatography of material partially digested with nuclease P1, (b) two-dimensional polyacrylamide gelelectrophoresis of material partially digested with alkali, and (c) one-dimensional gelelectrophoresis of material partially digested with RNase TI, RNase U2 and alkali. The details of these procedures have been described (6,7).…”

Section: Van Boom (Leiden)mentioning

confidence: 99%

“…Previously, we reported the sequence of an MW 29,000-fragment (fragment 29) of AlMV RNA 4 that contains the coat protein binding site (6). This fragment was found to represent the 3'terminal 91 nucleotides of RNA 4.…”

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Nucleotide sequence of the 3′-noncoding region of alfalfa mosaic virus RNA 4 and its homology with the genomic RNAs

Koper-Zwarthoff¹,

Brederod²,

Walstra³

et al. 1979

Nucl Acids Res

View full text Add to dashboard Cite

A 226-nucleotide fragment was derived from alfalfa mosaic virus RNA 4 (ALMV RNA 4), the subgenomic messenger for viral coat protein, and its sequence was deduced by in vitro labeling with polynucleotide kinase and application of RNA sequencing techniques. The fragment contains the 3'-terminal 45 nucleotides of the coat protein cistron and the complete 3'-noncoding region of 182 nucleotides. The total length of RNA 4 was calculated to be 881 nucleotides. AlMV RNAs 1, 2 and 3 were elongated with a 3'-terminal poly(A) stretch and subjected to sequence analysis by using a specific primer, reverse transcriptase and chain terminators. This revealed and extensive homology between the 3'-terminal 140 to 150 nucleotides of all four ALMV RNAs. Despite a number of base substitutions, the secondary structure of the homologous region is highly conserved. The observed homology indicates that, as with RNA 4, the sites with a high affinity for the viral coat protein are located at the 3'-termini of the genomic RNAs.

show abstract

Section: Discussionmentioning

confidence: 56%

Section: Van Boom (Leiden)mentioning

confidence: 99%

Section: Van Boom (Leiden)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations