Sequence homology at the 3′‐ends of alfalfa mosaic virus RNAs

Pinck, L.; Pinck, Monique

doi:10.1016/0014-5793(79)80463-9

Cited by 31 publications

(12 citation statements)

References 16 publications

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“…The 3'-terminal noncoding sequence consists of 179 bases (Fig. 4), and is similar to all of the published data on this region (17,26,30,31). The three base substitutions found in this area do not affect any of the postulated secondary structures for coat protein binding (32,33).…”

supporting

confidence: 83%

Complete nucleotide sequence of alfalfa mosaic virus RNA3

et al. 1983

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A full-length cDNA clone of alfalfa mosaic virus (AMV) RNA3 was prepared and sequenced. The 2,037 base sequence contains two open reading frames of 903 and 666 nucleotides that code for a 32,400 dalton protein (32.4K protein) and the 24,380 dalton coat protein, respectively. A 5'-noncoding sequence of 240 bases preceeding the 32.4K protein contains homologous regions that may have a function in its translation. The intercistronic junction is 49 bases long, the last 36 bases representing the 5'-end of the subgenomic RNA4. The remaining 179 bases comprise the 3'-terminal noncoding sequence.

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supporting

confidence: 83%

Complete nucleotide sequence of alfalfa mosaic virus RNA3

et al. 1983

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“…Hybridization analysis which is much less affected by minor sequence differences revealed slight (on average < 10%) homology between the individual RNAs of each virus. This low level of hybridization could arise from common 3'-and 5'-terminal sequences, similar to those of multicomponent plant viruses, probably involved in RNA polymerase, coat protein and ribosome recognition (Symons, 1979;Pinck & Pinck, 1979;Gunn & Symons, 1980;Gould & Symons, 1977).…”

Section: Discussionmentioning

confidence: 99%

A Satellite Double-stranded RNA in a Virus from Gaeumannomyces graminis

Romanos

Buck

Rawlinson

1981

Journal of General Virology

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SUMMARYThe double-stranded (ds)RNA components of two serologically related viruses (019/6-A and 38-4-A), obtained from Gaeumannomyces graminis, have been compared. 019/6-A virus has two RNA components, dsl and ds2, whereas 38-4-A virus has three RNA components , ds 1, ds2 and ds3. T1 oligonucleotide fingerprint analysis and saturation hybridization experiments indicated that 019/6-A dsl and 38-4-A dsl were very similar, whereas 019/6-A ds2 and 38-4-A ds2 were about 50% homologous. 38-4-A ds3 had little homology with dsl or ds2 of either virus. The single-stranded (ss)RNA transcripts of 38-4-A virus dsl, ds2 and ds3 each directed the synthesis in vitro of a single polypeptide, with mol. wt. 62000, 55000 and 52000 respectively. Peptide mapping showed that these three polypeptides were unrelated in sequence and that ds2 encoded the virus capsid polypeptide. The ds3 is probably not required for the replication of 38-4-A virus and may be regarded as a satellite RNA.

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“…This phenomenon, which differentiates ilarviruses from bromoand cucumoviruses, is studied in most detail with AlMV. The ge- (10)(11)(12) have shown that coat protein binds preferentially to a region in the 3'-terminal sequence of 145 nucleotides that is highly homologous in RNAs 1, 2, and 3 (13)(14)(15). The hypothesis has been put forward by Houwing and Jaspars (16) that binding of coat protein to the 3'-termini of the genome segments is required for a proper recognition of the viral RNAs by the viral replicase.…”

Section: Introductionmentioning

confidence: 99%

Complete nucleotide sequence of tobacco streak virus RNA 3

et al. 1984

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Double-stranded cDNA of in vitro polyadenylated tobacco streak virus (TSV) RNA 3 has been cloned and sequenced. The complete primary structure of 2,205 nucleotides reveals two open reading frames flanked by a leader sequence of 210 bases, an intercistronic region of 123 nucleotides and a 3'-extracistronic sequence of 288 nucleotides. The 5'-terminal open reading frame codes for a Mr 31,742 protein, which probably corresponds to the only in vitro translation product of TSV RNA 3. The 3'-terminal coding region predicts a Mr 26,346 protein, probably the viral coat protein, which is the translation product of the subgenomic messenger, RNA 4. Although the coat proteins of alfalfa mosaic virus (A1MV) and TSV are functionally equivalent in activating their own and each others genomes, no homology between the primary structures of those two proteins is detectable.

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Sequence homology at the 3′‐ends of alfalfa mosaic virus RNAs

Cited by 31 publications

References 16 publications

Complete nucleotide sequence of alfalfa mosaic virus RNA3

Complete nucleotide sequence of alfalfa mosaic virus RNA3

A Satellite Double-stranded RNA in a Virus from Gaeumannomyces graminis

Complete nucleotide sequence of tobacco streak virus RNA 3

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