3’UTR of SARS-CoV-2 spike gene hijack host miR-296 or miR-520h to disturb cell proliferation and cytokine signaling

Yuan, Jinjin; Feng, Ziheng; Wang, Qiaowen; Han, Lizhen; Guan, Shenchan; Liu, Lijuan; Ye, Hanhui; Xu, Lili; Han, Xiao

doi:10.3389/fimmu.2022.924667

Cited by 6 publications

(1 citation statement)

References 51 publications

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“…The mechanism could be an interesting topic to further study as many infected people complained about changes in libido as a symptom of long COVID 9 . It was reported that SARS-CoV-2 could hijack the host mRNA and translation system by adjusting host immunity 56 , 57 . Hypothetically, prolactin first increased to regulate cellular immunity and humoral immunity 27 , 28 , and went down along with the recovery condition but took a long time.…”

Section: Discussionmentioning

confidence: 99%

Long-term effects of Omicron BA.2 breakthrough infection on immunity-metabolism balance: a 6-month prospective study

Li,

Qin,

Dong

et al. 2024

Nat Commun

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There have been reports of long coronavirus disease (long COVID) and breakthrough infections (BTIs); however, the mechanisms and pathological features of long COVID after Omicron BTIs remain unclear. Assessing long-term effects of COVID-19 and immune recovery after Omicron BTIs is crucial for understanding the disease and managing new-generation vaccines. Here, we followed up mild BA.2 BTI convalescents for six-month with routine blood tests, proteomic analysis and single-cell RNA sequencing (scRNA-seq). We found that major organs exhibited ephemeral dysfunction and recovered to normal in approximately six-month after BA.2 BTI. We also observed durable and potent levels of neutralizing antibodies against major circulating sub-variants, indicating that hybrid humoral immunity stays active. However, platelets may take longer to recover based on proteomic analyses, which also shows coagulation disorder and an imbalance between anti-pathogen immunity and metabolism six-month after BA.2 BTI. The immunity-metabolism imbalance was then confirmed with retrospective analysis of abnormal levels of hormones, low blood glucose level and coagulation profile. The long-term malfunctional coagulation and imbalance in the material metabolism and immunity may contribute to the development of long COVID and act as useful indicator for assessing recovery and the long-term impacts after Omicron sub-variant BTIs.

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Section: Discussionmentioning

confidence: 99%

Long-term effects of Omicron BA.2 breakthrough infection on immunity-metabolism balance: a 6-month prospective study

Li,

Qin,

Dong

et al. 2024

Nat Commun

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Cardiac miR-19a/19b was induced and hijacked by CVB3 to facilitate virus replication via targeting viral genomic RdRp-encoding region

Wu,

Yue,

Xiong

2023

Antiviral Research

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Cellular microRNAs target SARS-CoV-2 spike protein and restrict viral replication

Vaddadi

Gandikota

Huang

et al. 2023

American Journal of Physiology-Cell Physiology

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MicroRNAs (miRNAs) regulate gene expression post-transcriptionally and are implicated in viral replication and host tropism. miRNAs can impact the viruses either by directly interacting with the viral genome or modulating host factors. Although many miRNAs have predicted binding sites in the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) viral RNA genome, little experimental validation has been done. We first identified 492 miRNAs that have binding site(s) on the spike (S) viral RNA by a bioinformatics prediction. We then validated the selected 39 miRNAs by examining S-protein levels after co-expressing the S-protein and a miRNA into the cells. Seven miRNAs were found to reduce the S-protein levels more than 50%. Among them, miR-15a, miR-153, miR-298, miR-508, miR-1909 and miR-3130 also significantly reduced SARS-CoV-2 viral replication. SARS-CoV-2 infection decreased the expression levels of miR-298, miR-497, miR-508, miR-1909, and miR-3130, but had no significant effects on miR-15a and miR-153 levels. Intriguingly, the targeting sequences of these miRNAs on the S viral RNA showed sequence conservation among the variants of concern. Our results suggest that these miRNAs elicit effective antiviral defense against SARS-CoV-2 by modulating S-protein expression and are likely targeting all the variants. Thus, the data signifies the therapeutic potential of miRNA-based therapy for SARS-CoV-2 infections.

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3’UTR of SARS-CoV-2 spike gene hijack host miR-296 or miR-520h to disturb cell proliferation and cytokine signaling

Cited by 6 publications

References 51 publications

Long-term effects of Omicron BA.2 breakthrough infection on immunity-metabolism balance: a 6-month prospective study

Long-term effects of Omicron BA.2 breakthrough infection on immunity-metabolism balance: a 6-month prospective study

Cardiac miR-19a/19b was induced and hijacked by CVB3 to facilitate virus replication via targeting viral genomic RdRp-encoding region

Cellular microRNAs target SARS-CoV-2 spike protein and restrict viral replication

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