322. The reactions of substituted maleimides with thiols

Marrian, D. H.

doi:10.1039/jr9490001515

Cited by 49 publications

(27 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…N-ethylmaleimide (NEM) in the same concentration at pH 5.6 also inactivated the pyrogen. The reaction of NEM with sulfhydryl groups is known to be irreversible (30), a fact confirmed in other experiments with the pyrogen not recorded in Table V. Additional experiments not recorded in the table dealt with pCMPS reactions at pH 7, and variations in the duration and temperature of the first incubation period.…”

Section: Alkaline Inactivation and The Requirement For Free Sulfhydrysupporting

confidence: 65%

Studies on the Pathogenesis of Fever

Kozak

Hahn

Lennarz

et al. 1968

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

Section: Alkaline Inactivation and The Requirement For Free Sulfhydrysupporting

confidence: 65%

Studies on the Pathogenesis of Fever

Kozak

Hahn

Lennarz

et al. 1968

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…[19] The low molecular weight maleimide 11a and L -glutathione ( 12 ) were used as models for a maleimide-thiol conjugation reaction. Since both of these molecules are small, the reaction progress could be easily monitored directly by 1 H NMR spectroscopy (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Maleimide Functionalized Poly(ε‐caprolactone)‐block‐poly(ethylene glycol) (PCL‐PEG‐MAL): Synthesis, Nanoparticle Formation, and Thiol Conjugation

Zhu

Hoye

et al. 2009

Macro Chemistry & Physics

View full text Add to dashboard Cite

SummaryCarboxylic acid terminated poly(ε-caprolactone)s (PCL-COOHs) with narrow polydispersity were synthesized and coupled with poly(ethylene glycol) (HO-PEG-OH) to afford PCL-PEG-OH copolymers. The hydroxyl groups in the PCL-PEG-OHs were then converted to maleimide groups to afford maleimide terminated PCL-PEG-MALs that contained 70-90% maleimide functionality. Nanoparticles with maleimide functionality on their surfaces were prepared by impingement mixing. Particle sizes and size distributions were determined by dynamic light scattering. Conjugation of reduced glutathione with model maleimides and two MAL-functional nanoparticles was also demonstrated. The amount of accessible maleimide on the particle surface was measured using Ellman's reagent to range between ~51-67%.

show abstract

“…Figure 3, curve D, shows that with wool pretreated for 4 days at 30° C. in 0.001 M mercuric chloride (wool to liquor ratio 1:1000 ) , there was very little reaction. Tests on wool pretreated for 10 days at 30° C. in a 0.05 M solution of N-ethylmaleimide [8] in pH 7.4 phosphate buffer (wool to liquor ratio 1:250) were less satisfactory. Curve E shows that after 14 days 8 micromoles of the reagent had reacted per gram of this wool, which therefore probably contained sulfhydryl groups inaccessible to aqueous N-ethylmaleimide [ 14] .…”

Section: The Penetration Of the Reagent Into Wool Fibersmentioning

confidence: 90%

The Estimation of Sulfhydryl Groups in Wool by Means of 1-(4-Chloromercuriphenylazo)- Naphthol-2

Burley

1956

Textile Research Journal

View full text Add to dashboard Cite

IT has recently been reported [4] that sulfhydryl groups in wool fibers may be detected and estimated by means of a formamide solution of Bennett's color reagent, 1-(4-chloromercuriphenylazo ) -naphthol-2 [ 1,15,16] . Further experiments have now shown that the acidity and water content of the formamide are important in determining the rate and extent of penetration of this reagent into the fiber, and that the values reported earlier are therefore too low. This communication gives directions for increasing the reliability of the method for use on intact fibers and describes the standardization of the reagent on known sulfhydryl compounds. A subsequent communication will describe how the method can be shortened by using supercontracted and pulverized wool. A brief outline of the methods, without experimental details, has already been given [5]. Materials WoolThe wool samples used, which unless otherwise stated, were from the root ends of one good quality South African merino fleece (from sheep B51.3), were purified by Soxhlet-extraction for 24 hr. with hexane, then for 12 hr. with ethyl alcohol, followed by soaking for 24 hr. in frequently changed distilled water.

show abstract

322. The reactions of substituted maleimides with thiols

Cited by 49 publications

References 0 publications

Studies on the Pathogenesis of Fever

Studies on the Pathogenesis of Fever

Maleimide Functionalized Poly(ε‐caprolactone)‐block‐poly(ethylene glycol) (PCL‐PEG‐MAL): Synthesis, Nanoparticle Formation, and Thiol Conjugation

The Estimation of Sulfhydryl Groups in Wool by Means of 1-(4-Chloromercuriphenylazo)- Naphthol-2

Contact Info

Product

Resources

About