329 a Novel Suspension Culture System for Bovine Oviduct Epithelial Cells

Rottmayer, R.; Ulbrich, Susanne E.; Koelle, Sabine; Prelle, Katja; Meyer, H. H. D.; Sinowatz, Fred; Wolf, Eckhard; Hiendleder, Stefan

doi:10.1071/rdv17n2ab329

Reprod. Fertil. Dev.

2005

DOI: 10.1071/rdv17n2ab329

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329 a Novel Suspension Culture System for Bovine Oviduct Epithelial Cells

R. Rottmayer

Susanne E. Ulbrich²,

Sabine Koelle

et al.

Abstract: We previously isolated epithelial-like cells (ELC) from sheep (Ovis aries) and eland (Taurotragus oryx) ejaculates (Nel-Themaat et al. 2004 Reprod. Fertil. Dev. 16, 152). Success rates were low, and the presence of live sperm during initial culture may have altered medium properties, physically

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Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Ulbrich¹,

Rehfeld²,

Bauersachs³

et al. 2006

Journal of Endocrinology

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Nitric oxide synthases (NOS) account for the endogenous production of nitric oxide (NO), a small and permeable bioreactive molecule. NO is known to act as a paracrine mediator during various processes associated with female reproduction. In the present study, the mRNA expression of the endothelial (eNOS) and inducible (iNOS) NO synthases were examined in bovine oviduct epithelial cells (BOEC) during the oestrous cycle. In addition, eNOS and iNOS mRNA and protein were localised by in situ hybridisation and immunocytochemistry respectively. Furthermore, the effects of exogenously applied oestradiol-17 and progesterone on NOS mRNA regulation were studied in a suspension culture of BOEC. The eNOS mRNA abundance was low around ovulation (day 0) and increased significantly until pro-oestrus (day 18) in the ampulla. Immunoreactive protein of eNOS was detected predominantly in endothelial cells as well as in secretory oviduct epithelial cells at pro-oestrus. The iNOS mRNA concentration was significantly reduced in the isthmus at pro-oestrus (day 18) and oestrus (day 0) compared with persistently high levels in the ampulla. By in situ hybridisation, specific iNOS transcripts were additionally demonstrated in the oviduct epithelium. Immunoreactive iNOS protein was localised in secretory epithelial cells as well as in the lamina muscularis. The in vitro stimulation showed that both NOS were stimulated by progesterone, but not by oestradiol-17 . The region-specific modulated expression of eNOS and iNOS provides evidence for an involvement of endogenously produced NO in the regulation of oviductal functions.

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Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Ulbrich¹,

Rehfeld²,

Bauersachs³

et al. 2006

Journal of Endocrinology

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show abstract

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329 a Novel Suspension Culture System for Bovine Oviduct Epithelial Cells

Cited by 1 publication

References 0 publications

Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

Region-specific expression of nitric oxide synthases in the bovine oviduct during the oestrous cycle and in vitro

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