Virus diseases, which are one of the most important factor affecting yield and quality in garlic cultivation and without chemical control, cause more devastating results than other disease factors. Viruses of the genus Allexivirus, Potyvirus, Potexvirus, Carlavirus and Tospovirus are problematic in garlic production and the viruses are collectively called as Garlic Viral Complex (GVC). The most effective way to prevent viral diseases is to control viruses. For this purpose, the use of virus-free garlic can prevent the spread of the disease, as it prevents the initial contamination. Producing virus-free garlic plants through conventional agronomic systems is expensive and difficult. To overcome these problems, in vitro propagation of virus-free plants by meristem culture is a promising method to produce virus-free garlic in a short time, especially when combined with applications such as thermotherapy and chemotherapy. In this study, the application of meristem culture and the effects of pre-treatments used in the production of virus-free garlic, such as nutrient medium, thermotherapy and chemotherapy applied before or during the culture was investigated.